Photoluminescent Detection of Human T-Lymphoblastic Cells by ZnO Nanorods.

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RESEARCH PRODUCT

Photoluminescent Detection of Human T-Lymphoblastic Cells by ZnO Nanorods.

Yuliya Harmaza Ekaterina Slobozhanina Alexander Tamashevski Igor Iatsunskyi Roman Viter Roman Viter

subject

room temperature photoluminescence T-Lymphocytes Pharmaceutical Science 02 engineering and technology Biosensing Techniques T-lymphoblasts detection 01 natural sciences Analytical Chemistry hemic and lymphatic diseases Drug Discovery education.field_of_study Nanotubes medicine.diagnostic_test Antibodies Monoclonal Precursor Cell Lymphoblastic Leukemia-Lymphoma 021001 nanoscience & nanotechnology Flow Cytometry Chemistry (miscellaneous)Molecular Medicine Nanorod Zinc Oxide 0210 nano-technology monoclonal antibody anti-CD5 Photoluminescence Materials science Population chemistry.chemical_element Nanotechnology Zinc CD5 Antigens Article Flow cytometry lcsh:QD241-441 Adsorption lcsh:Organic chemistry Cell Line Tumor medicine Humans Physical and Theoretical Chemistry education MOLT-4 cell line cluster of differentiation proteins 010401 analytical chemistry Organic Chemistry 0104 chemical sciences Nanostructures chemistry Cancer cell Luminescent Measurements Glass Biosensor zinc oxide nanorods

description

The precise detection of cancer cells currently remains a global challenge. One-dimensional (1D) semiconductor nanostructures (e.g., ZnO nanorods) have attracted attention due to their potential use in cancer biosensors. In the current study, it was demonstrated that the possibility of a photoluminescent detection of human leukemic T-cells by using a zinc oxide nanorods (ZnO NRs) platform. Monoclonal antibodies (MABs) anti-CD5 against a cluster of differentiation (CD) proteins on the pathologic cell surface have been used as a bioselective layer on the ZnO surface. The optimal concentration of the protein anti-CD5 to form an effective bioselective layer on the ZnO NRs surface was selected. The novel biosensing platforms based on glass/ZnO NRs/anti-CD5 were tested towards the human T-lymphoblast cell line MOLT-4 derived from patients with acute lymphoblastic leukemia. The control tests towards MOLT-4 cells were performed by using the glass/ZnO NRs/anti-IgG2a system as a negative control. It was shown that the photoluminescence signal of the glass/ZnO NRs/anti-CD5 system increased after adsorption of T-lymphoblast MOLT-4 cells on the biosensor surface. The increase in the ZnO NRs photoluminescence intensity correlated with the number of CD5-positive MOLT-4 cells in the investigated population (controlled by using flow cytometry). Perspectives of the developed ZnO platforms as an efficient cancer cell biosensor were discussed.

year	journal	country	edition	language
2020-07-10	Molecules (Basel, Switzerland)

10.3390/molecules25143168 https://pubmed.ncbi.nlm.nih.gov/32664437