Search results for "C4"

Autoantivielu profils asins serumā pacientiem ar Sistēmisko sarkano vilkēdi, kas ārstējušīes Paula Stradiņa klīniskās universitātes slimnīcas Reimato…

2022

Sistemiskā sarkanā vilkēde ir hroniska autoimūna slimība, kas pieder pie retām reimatiskām slimībām. Tā raksturojas ar autoantivielu un to veidoto imūnkompleksu izraisītu audu bojājumu. Sistēmiskai sarkanai vilkēdei ir daudzpusīgas izpausmes ar daudzu orgānu iesaisti un tieši tādēļ, tā bieži paliek nediagnosticēta, kas kavē pacienta pareizās ārstēšanas terapijas uzsākšanu. Līdz ar to īpaša uzmanība ir jāpievērš imunoloģisko rādītāju izmaiņām, lai savlaicīgi varētu diagnosticēt šo slimību. Pētījuma mērķis. Retrospektīvi izpētīt un noskaidrot imunoloģisko rādītāju profīlu izmaiņas pacientiem ar Sistēmisko sarkano vilkēdi. Materiāli un metodes. Šis retrospektīvais pētījums tika veikts Paula St…

Dažādas izcelsmes medus autentiskuma novērtējums

2018

Darbā ir apkopota informācija par medu, tā viltošanu un viltojumu noteikšanas metodēm. Pētītas publikācijas par proteīnu izdalīšanu, vieglo stabilo izotopu masspektrometriju un aplūkotas hromatogrāfijas metodes cukuru noteikšanai. Tika veikta proteīnu izdalīšana no dažādas izcelsmes un viltotu medus paraugiem. Tika veikta vieglo stabilo izotopu attiecību analīze medus paraugos un viltošanai izmantotajām piedevām. Atsevišķi tika pētīti ar hromatogrāfijas metodi viltoti medus paraugi. Iegūtie rezultāti tika izmantoti, lai pētītu atšķirības starp dažādu reģionu izcelsmes medus paraugiem, kā arī, lai atrastu liecības par apzināti viltotiem medus paraugiem un pārliecinātos par to autentiskumu.

Worsening of Preexisting Psychiatric Conditions During the COVID-19 Pandemic

2020

Objectives: To ascertain factors associated with worsening of psychiatric conditions during the coronavirus disease 2019 (COVID-19) pandemic. Methods: This study anonymously examined 2,734 psychiatric patients worldwide for worsening of their preexisting psychiatric conditions during the COVID-19 pandemic. An independent clinical investigation of 318 psychiatric patients from United States was used for verification. Results: Valid responses mainly from 12 featured countries indicated self-reported worsening of psychiatric conditions in two-thirds of the patients assessed that was through their significantly higher scores on scales for general psychological disturbance, posttraumatic stress …

cIAP1 regulates TNF-mediated cdc42 activation and filopodia formation

2013

International audience; umour necrosis factor-α (TNF) is a cytokine endowed with multiple functions, depending on the cellular and environmental context. TNF receptor engagement induces the formation of a multimolecular complex including the TNFR-associated factor TRAF2, the receptor-interaction protein kinase RIP1 and the cellular inhibitor of apoptosis cIAP1, the latter being essential for NF-κB activation. Here, we show that cIAP1 also regulates TNF-induced actin cytoskeleton reorganization through a cdc42-dependent, NF-κB-independent pathway. Deletion of cIAP1 prevents TNF-induced filopodia and cdc42 activation. The expression of cIAP1 or its E3-ubiquitin ligase-defective mutant restore…

Overexpression of Ogg1 in mammalian cells: effects on induced and spontaneous oxidative DNA damage and mutagenesis

1999

Chinese hamster ovary cell lines (AA8 and AS52) were stably transfected to overexpress hOgg1 protein, the human DNA repair glycosylase for 7,8-dihydro-8-oxoguanine (8-oxoG). In the transfectants, the repair rate of 8-oxoG residues induced by either potassium bromate or the photosensitizer [R]-1-[(10-chloro-4-oxo-3-phenyl-4H-benzo[a]quinolizin-1-yl)-carbo nyl ]-2-pyrrolidinemethanolplus light was up to 3-fold more rapid than in the parental cells. However, the improved repair had little effect on the mutagenicity of potassium bromate in the guanine phosphoribosyl transferase (gpt) locus of the OGG1-transfected AS52 cells. The steady-state (background) levels of DNA base modifications sensiti…

Study of the role of the CDC48 chaperone protein in plant immunity

2018

The chaperone protein CDC48 (Cell division cycle 48) is a major regulator of the quality control of proteins and is involved in various cellular processes in animals and yeast. In contrast, the role of CDC48 in plants is poorly known. In the present work, we investigated the function of CDC48 in plant immunity thanks to the cryptogein/tobacco biological model, cryptogein being produced by the oomycete phytophthora cryptogea.Three strategies were carried out. First, the dynamic of accumulation CDC48 together with intracellular events inherent to the immune response were analyzed in both wild-type and CDC48 overexpressing tobacco cells (CDC48-TAP line). Second, a list if CDC48 partners was es…

Etude du rôle de la protéine CDC48 dans l'immunité des plantes

2018

The chaperone protein CDC48 (Cell division cycle 48) is a major regulator of the quality control of proteins and is involved in various cellular processes in animals and yeast. In contrast, the role of CDC48 in plants is poorly known. In the present work, we investigated the function of CDC48 in plant immunity thanks to the cryptogein/tobacco biological model, cryptogein being produced by the oomycete phytophthora cryptogea.Three strategies were carried out. First, the dynamic of accumulation CDC48 together with intracellular events inherent to the immune response were analyzed in both wild-type and CDC48 overexpressing tobacco cells (CDC48-TAP line). Second, a list if CDC48 partners was es…

The chaperone-like protein Cdc48 regulates ubiquitin-proteasome system in plants

2021

Emerging functions of nitric oxide in plant immunity

2016

SPEIPMUBAgrosupCNRS; The importance of nitric oxide (NO) in innate and adaptive immunity in mammals is well recognised. NO exerts antimicrobial properties against invaders but also displays immunoregulatory functions in which S-nitrosylation represents a signalling process of major importance. Over the last two decades, a growing body of evidence suggests that NO is also a major component of plant immunity. Our understanding of its role in plant defence has been enriched by the identification and functional analysis of S-nitrosylated proteins. The recent identification of new S-nitrosylated proteins including the chaperone-like enzyme cell division cycle 48 (CDC48), histone deacetylases (HD…

Traumatic experiences in childhood and psychopathy: a study on a sample of violent offenders from Italy

2013

Background: The link between early traumatic experiences of abuse/neglect and criminal behaviour has been widely demonstrated. Less is known, however, about the relationship between these experiences and the development of psychopathic personality. Objective: This study investigated childhood relational trauma in a group of violent offenders from Italy. We hypothesised a higher level of early relational trauma associated with higher scores on psychopathy. Method: Twenty-two offenders convicted for violent crimes aged 22-60 (M=38, SD=11) participated in this study. Participants were selected by the Italian justice system for an experimental research programme aiming at the evaluation of psyc…