Search results for "C70"

showing 10 items of 98 documents

Sea urchin coelomocytes as a novel cellular biosensor of environmental stress: a field study in the Tremiti Island Marine Protected Area, Southern Ad…

2007

The aim of the present study was to investigate on the suitability of the sea urchin as a sentinel organism for the assessment of the macro-zoobenthos health state in bio-monitoring programmes. A field study was carried out during two oceanographic campaigns using immuno-competent cells, the coelomocytes, from sea urchins living in a marine protected area. In particular, coelomocytes subpopulations ratio and heat shock protein 70 (HSC70) levels were measured in specimens of Paracentrotus lividus (Lamark, 1816) collected in two sampling sites, namely Pianosa and Caprara Islands, both belonging to the Tremiti Island Marine Protected Area (MPA) in the Southern Adriatic Sea, Italy. By density g…

Health Toxicology and MutagenesisBiosensing TechniquesEnvironmentBiologyToxicologyEnvironmental stressParacentrotus lividusStress Physiologicalbiology.animalAnimalsSeawaterGonadsCoelomocyteSea urchinAtomic emission spectrometryGeographyHSC70 Heat-Shock ProteinsCell Biologybiology.organism_classificationTrace ElementsFisheryOn boardPhenotypeItalyMetalsSea UrchinsMarine protected areaProtected areaBiomarkersWater Pollutants ChemicalCell Biology and Toxicology
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Sequence-Specific Repression of Cotranslational Translocation of the Hepatitis B Virus Envelope Proteins Coincides with Binding of Heat Shock Protein…

1997

AbstractThe large L envelope protein of the hepatitis B virus has the peculiar capacity to adopt two transmembrane topologies. The N-terminal preS domain of L initially remains in the cytosol while the S domain is cotranslationally inserted into the endoplasmic reticulum membrane. The preS region of about half of the L molecules is posttranslationally translocated to the lumenal space. We now demonstrate that the repression of cotranslational translocation of preS is conferred by a preS1-specific sequence. By analysis of L deletion mutants, the cytosolic anchorage determinant was mapped to amino acid sequence 70 to 94 of L. The intrinsic potential of this determinant to suppress cotranslati…

Hepatitis B virusHSC70 Heat-Shock ProteinsRecombinant Fusion ProteinsPlasma protein bindingBiologyGenes envCytosolViral Envelope ProteinsHeat shock proteinVirologyHumansHSP70 Heat-Shock ProteinsBinding sitePromoter Regions GeneticPeptide sequenceBinding SitesBase SequenceCell-Free SystemEndoplasmic reticulumHSC70 Heat-Shock ProteinsOligonucleotides AntisenseMolecular biologyTransmembrane proteinChaperone (protein)Protein Biosynthesisbiology.proteinMutagenesis Site-DirectedMetallothioneinCarrier ProteinsProtein BindingVirology
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Chaperones Involved in Hepatitis B Virus Morphogenesis

1999

Little is known about host cell factors necessary for hepatitis B virus (HBV) assembly which involves envelopment of cytosolic nucleocapsids by the S, M and L transmembrane viral envelope proteins and subsequent budding into intraluminal cisternae. Central to virogenesis is the L protein that mediates hepatocyte receptor binding and envelopment of capsids. To serve these topologically conflicting roles, L protein exhibits an unusual dual membrane topology, disposing its N-terminal preS domain inside and outside of the virion lipid envelope. The mixed topology is achieved by posttranslational preS translocation of about half of the L protein molecules across a post-endoplasmic reticulum memb…

Hepatitis B virusProtein FoldingCalnexinHSC70 Heat-Shock ProteinsClinical BiochemistryBiochemistryViral Matrix ProteinsCytosolViral Envelope ProteinsViral envelopeCalnexinMorphogenesisAnimalsHumansHSP70 Heat-Shock ProteinsProtein PrecursorsMolecular BiologyHepatitis B Surface AntigensViral matrix proteinbiologyChemistryCalcium-Binding ProteinsHSC70 Heat-Shock ProteinsBiological TransportVirologyTransmembrane proteinCell biologyProtein BiosynthesisMembrane topologyChaperone (protein)COS Cellsbiology.proteinProtein foldingCarrier ProteinsMolecular ChaperonesBiological Chemistry
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Identification in the rat brain of a set of nuclear proteins interacting with H1° mRNA

2012

Synthesis of H1° histone, in the developing rat brain, is also regulated at post-transcriptional level. Regulation of RNA metabolism depends on a series of RNA-binding proteins (RBPs); therefore, we searched for H1° mRNA-interacting proteins. With this aim, we used in vitro transcribed, biotinylated H1° RNA as bait to isolate, by a chromatographic approach, proteins which interact with this mRNA, in the nuclei of brain cells. Abundant RBPs, such as heterogeneous nuclear ribonucleoprotein (hnRNP) K and hnRNP A1, and molecular chaperones (heat shock cognate 70, Hsc70) were identified by mass spectrometry. Western blot analysis also revealed the presence of cold shock domain-containing protein…

Heterogeneous nuclear ribonucleoproteinRNA-binding proteinRNA-binding proteinBiologyenvironment and public healthHeterogeneous-Nuclear RibonucleoproteinsMass SpectrometryHistonesSettore BIO/10 - BiochimicaAnimalsRNA MessengerNuclear proteinRats WistarSettore BIO/06 - Anatomia Comparata E CitologiaRibonucleoproteinMessenger RNAPIPPinGeneral NeuroscienceRibonucleoprotein particleHSC70 Heat-Shock ProteinsRNABrainCSD-C2Molecular biologyCell biologyRatsHistonebiology.proteinH1° mRNAPost-transcriptional gene regulation
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Hsp70 localizes differently from chaperone Hsc70 in mouse mesoangioblasts under physiological growth conditions

2008

Mouse A6 mesoangioblasts express Hsp70 even in the absence of cellular stress. Its expression and its intracellular localization were investigated under normal growth conditions and under hyperthermic stress. Immunofluorescence assays indicated that without any stress a fraction of Hsp70 co-localized with actin microfilaments, in the cell cortex and in the contractile ring of dividing cells, while the Hsc70 chaperone did not. Hsp70 immunoprecipitation assays confirmed that a portion of Hsp70 binds actin. Immunoblot assays showed that both proteins were present in the nucleus. After heat treatment Hsp70 and actin continued to co-localize in the leading edge of A6 cells but not on microfilame…

Hot TemperatureHistologyPhysiologyImmunoprecipitationHsp70 Hsc70 Mesoangioblastmacromolecular substancesMicrofilamentCell LineMiceStress PhysiologicalCell cortexAnimalsHumansHSP70 Heat-Shock ProteinsActinbiologyStem CellsHSC70 Heat-Shock ProteinsCell BiologyGeneral MedicineActinsGlomerular MesangiumHsp70Cell biologyCell cultureChaperone (protein)biology.proteinCell DivisionCytokinesisMolecular ChaperonesJournal of Molecular Histology
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The Hsc/Hsp70 Co-Chaperone Network Controls Antigen Aggregation and Presentation during Maturation of Professional Antigen Presenting Cells

2011

The maturation of mouse macrophages and dendritic cells involves the transient deposition of ubiquitylated proteins in the form of dendritic cell aggresome-like induced structures (DALIS). Transient DALIS formation was used here as a paradigm to study how mammalian cells influence the formation and disassembly of protein aggregates through alterations of their proteostasis machinery. Co-chaperones that modulate the interplay of Hsc70 and Hsp70 with the ubiquitin-proteasome system (UPS) and the autophagosome-lysosome pathway emerged as key regulators of this process. The chaperone-associated ubiquitin ligase CHIP and the ubiquitin-domain protein BAG-1 are essential for DALIS formation in mou…

Macromolecular AssembliesImmune CellsCellular differentiationImmunologyAntigen presentationAntigen-Presenting Cellslcsh:MedicineAntigen Processing and RecognitionMajor histocompatibility complexBiochemistryMiceMolecular Cell BiologyMHC class IAutophagyAnimalsHSP70 Heat-Shock ProteinsAntigensProtein Interactionslcsh:ScienceAntigen-presenting cellBiologyImmune ResponseCellular Stress ResponsesAntigen PresentationMultidisciplinarybiologylcsh:RHSC70 Heat-Shock ProteinsImmunityProteinsCell DifferentiationDendritic cellChaperone ProteinsUbiquitin ligaseCell biologyProteostasisbiology.proteinlcsh:QProtein MultimerizationResearch ArticlePLoS ONE
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Circadian rhythm of COPD symptoms in clinically based phenotypes. Results from the STORICO Italian observational study

2019

Abstract Background Chronic Obstructive Pulmonary Disease (COPD) encompasses various phenotypes that severely limit the applicability of precision respiratory medicine. The present investigation is aimed to assess the circadian rhythm of symptoms in pre-defined clinical COPD phenotypes and its association with health-related quality of life (HR-QoL), the quality of sleep and the level of depression/anxiety in each clinical phenotype. Methods The STORICO (NCT03105999) Italian observational prospective cohort study enrolled COPD subjects. A clinical diagnosis of either chronic bronchitis (CB), emphysema (EM) or mixed COPD-asthma (MCA) phenotype was made by clinicians at enrollment. Baseline e…

MaleChronic bronchitisTime FactorsHealth StatusAnxietyHospital Anxiety and Depression ScaleSeverity of Illness IndexHealth StatuPulmonary Disease Chronic Obstructive0302 clinical medicineSurveys and QuestionnairesForced Expiratory VolumeClinical phenotypeMedicineSurveys and QuestionnaireRespiratory function030212 general & internal medicineProspective StudiesProspective cohort studyLungDepression (differential diagnoses)COPDDepressionMiddle AgedCircadian RhythmPhenotypeItalyAnxietyFemalemedicine.symptom24-hour symptoms Clinical phenotype Real-world Respiratory functionResearch ArticleHumanPulmonary and Respiratory Medicinemedicine.medical_specialtyChronic ObstructiveTime FactorSettore MED/10 - Malattie Dell'Apparato RespiratorioPulmonary Disease03 medical and health sciencesInternal medicineHumansAsthma24-hour symptoms; Clinical phenotype; Real-world; Respiratory function; Aged; Anxiety; Depression; Female; Forced Expiratory Volume; Health Status; Humans; Italy; Lung; Male; Middle Aged; Phenotype; Prospective Studies; Pulmonary Disease Chronic Obstructive; Severity of Illness Index; Sleep; Surveys and Questionnaires; Time Factors; Circadian Rhythm; Quality of LifeAgedlcsh:RC705-77924-hour symptomsbusiness.industryCorrectionlcsh:Diseases of the respiratory systemmedicine.disease24-hour symptomProspective Studie030228 respiratory systemReal-worldQuality of LifebusinessSleepRespiratory function
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Disease awareness in patients with COPD: measurement and extent

2018

Ilaria Baiardini,1 Paola Rogliani,2 Pierachille Santus,3 Angelo G Corsico,4 Marco Contoli,5 Nicola Scichilone,6 Fabiano Di Marco,7 Patrizia Lessi,8 Carla Scognamillo,8 Giorgia Molinengo,9 Fabio Ferri,10 Vincenzo Patella,11 Giuseppe Fiorentino,12 Mauro Carone,13 Fulvio Braido14 1Department of Biomedical Sciences, Humanitas University, Milan, Italy; 2Respiratory Unit, Department of Experimental Medicine, University of Rome “Tor Vergata”, Rome, Italy; 3Department of Biomedical and Clinical Sciences (DIBIC), University of Milan, Milan, Italy; 4Department of Internal Medicine and Therapeutics, University of Pavia, Pavia, Italy; 5Department of Medical Sciences, University of F…

MaleHealth Knowledge Attitudes PracticeTime Factorspatient satisfactionSettore MED/10 - Malattie dell'Apparato RespiratorioDiseaseSeverity of Illness IndexPulmonary Disease Chronic Obstructive0302 clinical medicineCost of IllnessSurveys and QuestionnairesActivities of Daily Living80 and overawareness030212 general & internal medicineLungIllness BehaviorAged 80 and overPracticeCOPDHealth PolicyHealth KnowledgeGeneral MedicineMiddle AgedItalyFemalePublic HealthPulmonary and Respiratory MedicineChronic Obstructivemedicine.medical_specialtySocio-culturaleInternational Journal of Chronic Obstructive Pulmonary DiseasePulmonary DiseaseTreatment satisfactionIllness perceptions03 medical and health sciencesNegatively associatedAwareness; COPD; Patient satisfaction; Questionnaire; Activities of Daily Living; Aged; Aged 80 and over; Cost of Illness; Dyspnea; Female; Humans; Illness Behavior; Italy; Lung; Male; Middle Aged; Patient Compliance; Patient Satisfaction; Pulmonary Disease Chronic Obstructive; Reproducibility of Results; Severity of Illness Index; Surveys and Questionnaires; Time Factors; Awareness; Health Knowledge Attitudes Practice; Patient Acceptance of Health CaremedicineHumansCOPDIn patientAgedlcsh:RC705-779business.industryquestionnaireEnvironmental and Occupational HealthPublic Health Environmental and Occupational HealthAwareneReproducibility of Resultslcsh:Diseases of the respiratory systemPatient Acceptance of Health Caremedicine.diseaseCOPD; awareness; patient satisfaction; questionnaireawareness; COPD; patient satisfaction; questionnaire; Pulmonary and Respiratory Medicine; Health Policy; Public Health Environmental and Occupational HealthClinical trialDyspnea030228 respiratory systemClinical Trial ReportAttitudesPhysical therapyPatient CompliancePatient awarenessbusiness
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Gastritis and gastroesophageal reflux disease are strongly associated with non-allergic nasal disorders

2021

AbstractBackgroundGastroesophageal reflux disease (GERD) has been reported to be significantly associated with chronic rhinosinusitis, but the strength of the association is still debated.AimsTo evaluate the strength of the association between gastritis/GERD and non-allergic rhinitis (NAR)/allergic rhinitis (AR)/sinusitis.MethodsWe investigated 2887 subjects aged 20–84 years, who underwent a clinical visit in seven Italian centres (Ancona, Palermo, Pavia, Terni, Sassari, Torino, Verona) within the study on Gene Environment Interactions in Respiratory Diseases, a population-based multicase-control study between 2008 and 2014. Subjects were asked if they had doctor-diagnosed “gastritis or sto…

MaleNon-allergic rhinitisGastroesophageal reflux diseaseGastroenterologyAllergic rhinitis0302 clinical medicinePrevalenceNon-allergic rhiniti030223 otorhinolaryngologySinusitisRhinitisAged 80 and overeducation.field_of_studyMiddle AgedGastrititItalyGastritisGastroesophageal RefluxFemalePublic HealthGastritismedicine.symptomResearch ArticleAdultPulmonary and Respiratory Medicinemedicine.medical_specialtyPopulationGastrititsSettore MED/10 - Malattie Dell'Apparato RespiratorioNOHiatal herniaGastritits Gastroesophageal refux disease Allergic rhinitis Non-allergic rhinitis SinusitisYoung Adult03 medical and health sciencesAllergic rhinitis; Gastritits; Gastroesophageal reflux disease; Non-allergic rhinitis; SinusitisInternal medicineAllergic rhinitimedicineHumansGastroesophageal refux diseaseSinusitiseducationAgedlcsh:RC705-779business.industrylcsh:Diseases of the respiratory systemmedicine.diseaseRhinitis AllergicSinusitidigestive system diseases030228 respiratory systemRelative riskChronic DiseaseGERDAllergic rhinitis; Gastritits; Gastroesophageal reflux disease; Non-allergic rhinitis; Sinusitis Public HealthNon-allergic rhinitisbusinessEsophagitis
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Discriminative and predictive properties of disease-specific and generic health status indexes in elderly COPD patients

2008

Abstract Background The association between bronchial obstruction severity and mortality in Chronic Obstructive Pulmonary Disease (COPD) is well established, but it is unknown whether disease-specific health status measures and multidimensional assessment (MDA) have comparable prognostic value. Methods We analyzed data coming from the Salute Respiratoria nell'Anziano (Respiratory Health in the Elderly – SaRA) study, enrolling elderly people attending outpatient clinics for respiratory and non-respiratory problems. From this population we selected 449 patients with bronchial obstruction (77.3% men, mean age 73.1). We classified patients' health status using tertiles of the Saint George Respi…

MalePulmonary and Respiratory MedicineDisease specificmedicine.medical_specialtyCopd patientsPulmonary diseaseSettore MED/10 - Malattie Dell'Apparato RespiratorioSeverity of Illness IndexPulmonary Disease Chronic ObstructiveDiscriminative modelForced Expiratory VolumeSeverity of illnessmedicineHealth Status IndicatorsHumansCOPDIntensive care medicineSurvival analysisAgedlcsh:RC705-779COPDbusiness.industrylcsh:Diseases of the respiratory systemPrognosismedicine.diseaseSurvival AnalysisFemaleGeriatric Depression ScalebusinessResearch Article
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