Search results for "CAG"
showing 10 items of 492 documents
Influence of type III bacterial secretion system on the interactions between plant and non pathogenic fluorescent Pseudomonads spp.
2010
No abstract
Shotgun metagenomics sequencing of the rhizosphere microbiota associated to seven ecotypes of Medicago truncatula
2019
International audience; Healthy plants host a remarkable diversity of microorganisms known as plant microbiota, which provide host services such as pathogen protection and nutrient acquisition. Thus, plant microbiota emerges as a trait that extends the capacity of plants to adapt to their environment. So far, microbial community profiling has mostly allowed the description of the phylogenetic structure of plant microbiota, whereas functional insights were mostly obtained from experiments using model strains. Thus, the plant impact on the microbial functional genes pool in the rhizosphere remains largely unknown. The goals of the study were to (i) compare the functional genes pool of the rhi…
The Medicago truncatula hypermycorrhizal B9 mutant displays an altered response to phosphate and is more susceptible to Aphanomyces euteiches.
2014
SPE IPM; National audience; Inorganic phosphate (Pi) plays a key role in the development of arbuscular mycorrhizal (AM) symbiosis, which is favoured when Pi is limiting in the environment. We have characterized the Medicago truncatula hypermycorrhizal B9 mutant for its response to limiting (P/10) and replete (P2) Pi. On P2, mycorrhization was significantly higher in B9 plants than in wild-type (WT). The B9 mutant displayed hallmarks of Pi-limited plants, including higher levels of anthocyanins and lower concentrations of Pi in shoots than WT plants. Transcriptome analyses of roots of WT and B9 plants cultivated on P2 or on P/10 confirmed the Pi-limited profile of the mutant on P2 and highli…
Transcriptional response of Medicago truncatula sulphate transporters to arbuscular mycorrhizal symbiosis with and without sulphur stress
2013
Sulphur is an essential macronutrient for plant growth, development and response to various abiotic and biotic stresses due to its key role in the biosynthesis of many S-containing compounds. Sulphate represents a very small portion of soil S pull and it is the only form that plant roots can uptake and mobilize through H(+)-dependent co-transport processes implying sulphate transporters. Unlike the other organically bound forms of S, sulphate is normally leached from soils due to its solubility in water, thus reducing its availability to plants. Although our knowledge of plant sulphate transporters has been growing significantly in the past decades, little is still known about the effect of…
Use of translational genomics to identify genes important for legume seed development
2015
BAP Pôle GEAPSI; International audience; We have exploited the extensive synteny between the model legume Medicago truncatula and the cultivated garden pea, Pisumsativum, to identify loci controlling seed filling and seedcomposition in the crop species. QTLs for these traits are mapped by analyzing variation with in collections of recombinant inbred lines. Candidate genes with in the QTL intervals are identified by reference to the M.truncatula genomic sequence. This approach was used to uncover an endosperm subtilase that is associated with syntenic seed weight QTLs in Medicago and pea, and we discuss the possible role played by this enzyme in contributing to final seed weight.
Etude des interactions plantes-microbes et microbes-microbes au sein de la rhizosphère, sous un aspect coûts-bénéfices, dans un contexte de variation…
2013
Understanding the interactions that bind plants and soil microorganisms is an essential step for the sustainable management of ecosystems, especially in agriculture. The ecosystem services resulting from such interactions include plant productivity which responds, in part, to the food requirements of the world's population and the regulation of biogeochemical cycles. These ecosystem services depend on trophic links between the two partners in the interaction and can be represented by a tradeoff between the costs and benefits for each partner. Plants, being autotrophic organisms or primary producers, are key organisms which introduce carbon into the ecosystem, through photosynthesis. Part of…
Genetic determinism of the plasticity of legume seed protein in different environments : role of sulfur metabolism
2021
The renewed interest in plant proteins has stimulated research aimed at developing markers to aid in the selection of legume varieties better adapted to nutritional needs. Among the traits to be improved and stabilized is the amino acid balance of seeds, the essential amino acids methionine and tryptophan being particularly under-represented in legume seeds. This thesis focuses on seed protein composition, which is a major determinant of the seed amino acid balance. The objective was to explore the genetic and environmental variability in this trait and to identify genes potentially involved in its plasticity when subjected to environmental stresses. In the first part of the thesis, the pro…
The membrane proteome of Medicago truncatula roots displays qualitative and quantitative changes in response to arbuscular mycorrhizal symbiosis
2014
International audience; Arbuscular mycorrhizal (AM) symbiosis that associates roots of most land plants with soil-borne fungi (Glomeromycota), is characterized by reciprocal nutritional benefits. Fungal colonization of plant roots induces massive changes in cortical cells where the fungus differentiates an arbuscule, which drives proliferation of the plasma membrane. Despite the recognized importance of membrane proteins in sustaining AM symbiosis, the root microsomal proteome elicited upon mycorrhiza still remains to be explored. In this study, we first examined the qualitative composition of the root membrane proteome of Medicago truncatula after microsome enrichment and subsequent in dep…
Costs-benfits trade-off in the intercation between Medicago truncatula and Pseudomonas fluorescens C7R12 across atmospheric carbon dioxide modulation
2011
The interactions between plants and soil microorganisms are mainly based on trophic relationships. The compounds exchanged represent cost for the organism produces them but a benefit for the one who receive those compounds. A mutualistic relation leads to stability in the cost-benefit balance resulting from a co-evolution between the two organisms. The cost corresponding for the release of carbon compounds by the plant would be offset by benefits in return corresponding for the activities of microorganisms that use them. We tested by an experimental way the effect of CO2 concentration on the interaction between M. truncatula and the bacterium P. fluorescens C7R12. The results allowed a best…
Towards a cell-specific expression atlas of arbuscular mycorrhizal roots in the model legume Medicago truncatula
2007
International audience