Search results for "CD"

Nuevas aportaciones al conocimiento de macroinvertebrados de agua dulce de Costa Rica y Nicaragua, con especial énfasis en las esponjas

2018

Se estudia la caracterización limnológica de 30 lagunas del bosque tropical seco de Costa Rica y Nicaragua durante 2010-11. Se aislaron gémulas de esponjas de agua dulce en cuatro de las localidades. Se registraron dos nuevas citas de esponjas para Nicaragua, Radiospongilla cerebellata (Bowerbank, 1863) y Corvoheteromeyenia heterosclera (Ezcurra de Drago, 1974). En Costa Rica también se registró esta última y se recolectaron ejemplares de Radiospongilla crateriformis (Potts, 1882). En otra laguna de Costa Rica, se recolectó una larva de neuróptero (Insecta) de la familia Sisyridae asociada a las esponjas: Sisyra apicalis Banks, 1908. Por otro lado, hemos podido recolectar gémulas incrustada…

'Apparently, women don't know how to operate doors': a corpus-based analysis of women stereotypes in the tv series 3rd Rock from the Sun

2017

This paper explores how women stereotypes are discursively evaluated in the TV sitcom 3 rd Rock from the Sun by paying attention to the societal, cultural and ideological values they convey. Following recent trends for the study of television series (Bednarek, 2010), the analysis is both qualitative and quantitative, adopting a Corpus- Assisted Discourse Analysis approach (Baker, 2006; Partington, 2004). The contextualised analysis of words that refer to women confirms that the sitcom writers of 3 rd Rock from the Sun purposefully resort to stereotyping as a verbal strategy to create humour while conveying negative attitudes towards women.

Nanosecond pulsed electric field inhibits malignant melanoma growth by inducing the change of systemic immunity

2019

Background Nanosecond pulsed electric fields (nsPEFs) showed an inhibitory effect on proliferation of malignant melanoma. In this study, the growth of melanoma were inhibited by changing the systemic immunity. Material and Methods C57BL/6 mice with B16 malignant were exposed to 200 pulses of 100 ns duration, 30kV/cm. The mice were executed four days later. T lymphocyte has been extracted from spleen. Cell viability was evaluated by CCK-8 assay. CD3+CD4+ T cells, CD3+CD8+ T cells, regulatory T cells (Treg) and myeloid-derived suppressor cells (MDSC) were analyzed by flow cytometry. TNF-α, IL-2, IL-10, TGF-β, IFN– γ levels in supernatants were assessed by ELISA. Results C57 malignant melanoma…

Dacarbazine-mediated upregulation of NKG2D ligands on tumor cells activates NK and CD8 T cells and restrains melanoma growth.

2013

International audience; Dacarbazine (DTIC) is a cytotoxic drug widely used for melanoma treatment. However, the putative contribution of anticancer immune responses in the efficacy of DTIC has not been evaluated. By testing how DTIC affects host immune responses to cancer in a mouse model of melanoma, we unexpectedly found that both natural killer (NK) and CD8(+) T cells were indispensable for DTIC therapeutic effect. Although DTIC did not directly affect immune cells, it triggered the upregulation of NKG2D ligands on tumor cells, leading to NK cell activation and IFNγ secretion in mice and humans. NK cell-derived IFNγ subsequently favored upregulation of major histocompatibility complex cl…

Increased frequencies of CD11b+CD33+CD14+HLA-DRlowmyeloid-derived suppressor cells are an early event in melanoma patients

2014

Myeloid-derived suppressor cells (MDSC) are a heterogeneous cell population characterized by immunosuppressive activity. Elevated levels of MDSC in peripheral blood are found in inflammatory diseases as well as in malignant tumors where they are supposed to be major contributors to mechanisms of tumor-associated tolerance. We investigated the frequency and function of MDSC in peripheral blood of melanoma patients and observed an accumulation of CD11b(+) CD33(+) CD14(+) HLA-DR(low) MDSC in all stages of disease (I-IV), including early stage I patients. Disease progression and enhanced tumor burden did not result in a further increase in frequencies or change in phenotype of MDSC. By investig…

Altered intracellular sorting signals do not influence the efficacy of genetic melanoma vaccines incorporating helper determinants in mice.

2004

Background A genetic melanoma vaccine consisting of cDNA encoding the model self-antigen tyrosinase-related protein 2 (TRP2) fused in-frame to the immunogenic enhanced green fluorescent protein (EGFP) was able to break immune tolerance and stimulate CD8+ T cells in vivo. In the present study we investigated whether alteration of the intracellular antigen localization as a result of the linkage with immune-enhancing helper proteins affects the resulting immune response. Methods Expression plasmids and recombinant adenoviruses were constructed encoding various fusion proteins with different intracellular sorting signals which direct the antigen to the cytosol, the endoplasmic reticulum or the…

The mitotic kinase Aurora-A promotes distant metastases by inducing epithelial-to-mesenchymal transition in ERα+ breast cancer cells

2013

In this study, we demonstrate that constitutive activation of Raf-1 oncogenic signaling induces stabilization and accumulation of Aurora-A mitotic kinase that ultimately drives the transition from an epithelial to a highly invasive mesenchymal phenotype in estrogen receptor α-positive (ERα(+)) breast cancer cells. The transition from an epithelial- to a mesenchymal-like phenotype was characterized by reduced expression of ERα, HER-2/Neu overexpression and loss of CD24 surface receptor (CD24(-/low)). Importantly, expression of key epithelial-to-mesenchymal transition (EMT) markers and upregulation of the stemness gene SOX2 was linked to acquisition of stem cell-like properties such as the ab…

Social construction of quality in software development

2011

RIE : revista de investigación educativa

2018

Se revisa la evolución, situación actual y perspectivas de las revistas de investigación sobre educación, haciendo énfasis sobre las publicadas en español. Metodología: Se utiliza la estructura de la metodología DAFO (debilidades, amenazas, fortalezas y oportunidades), que ha demostrado ser una técnica muy eficaz para analizar y formular estrategias de acción que conduzcan a la mejora de un producto o servicio. Resultados: Se identifican las principales debilidades, amenazas, fortalezas y oportunidades de las revistas de investigación en educación, señalando los elementos fundamentales, internos y externos, que influyen sobre su desarrollo y calidad. Discusión: Se presentan conclusiones sob…

Quantum computing challenges in the software industry. A fuzzy AHP-based approach

2022

Context The current technology revolution has posed unexpected challenges for the software industry. In recent years, the field of quantum computing (QC) technologies has continued to grow in influence and maturity, and it is now poised to revolutionise software engineering. However, the evaluation and prioritisation of QC challenges in the software industry remain unexplored, relatively under-identified and fragmented. Objective The purpose of this study is to identify, examine and prioritise the most critical challenges in the software industry by implementing a fuzzy analytic hierarchy process (F-AHP). Method First, to identify the key challenges, we conducted a systematic literature rev…