6533b853fe1ef96bd12ac16d

RESEARCH PRODUCT

Increased frequencies of CD11b+CD33+CD14+HLA-DRlowmyeloid-derived suppressor cells are an early event in melanoma patients

Stephan GrabbeBerenice M. RudolphAlexander GerweCarmen LoquaiKerstin SteinbrinkAndrea TuettenbergNicole Bacher

subject

Skin Neoplasmsmedicine.medical_treatmentCD14Sialic Acid Binding Ig-like Lectin 3CD33PopulationLipopolysaccharide ReceptorsReceptors Antigen T-CellDermatologyBiologyLymphocyte ActivationT-Lymphocytes RegulatoryBiochemistryImmune toleranceTetanus ToxoidHLA-DRmedicineHumansMyeloid CellsLymphocyte CounteducationMelanomaMolecular BiologyCells CulturedCell ProliferationNeoplasm Stagingeducation.field_of_studyCD11b AntigenMelanomaInterleukin-8HLA-DR AntigensImmunotherapymedicine.diseaseCoculture TechniquesTumor BurdenCase-Control StudiesImmunologyDisease ProgressionLeukocytes MononuclearMyeloid-derived Suppressor CellTumor Escape

description

Myeloid-derived suppressor cells (MDSC) are a heterogeneous cell population characterized by immunosuppressive activity. Elevated levels of MDSC in peripheral blood are found in inflammatory diseases as well as in malignant tumors where they are supposed to be major contributors to mechanisms of tumor-associated tolerance. We investigated the frequency and function of MDSC in peripheral blood of melanoma patients and observed an accumulation of CD11b(+) CD33(+) CD14(+) HLA-DR(low) MDSC in all stages of disease (I-IV), including early stage I patients. Disease progression and enhanced tumor burden did not result in a further increase in frequencies or change in phenotype of MDSC. By investigation of specific MDSC-associated cytokines in patients' sera, we found an accumulation of IL-8 in all stages of disease. T-cell proliferation assays revealed that MDSC critically contribute to suppressed antigen-specific T-cell reactivity and thus might explain the frequently observed transient effects of immunotherapeutic strategies in melanoma patients.

yearjournalcountryeditionlanguage
2014-01-30Experimental Dermatology
https://doi.org/10.1111/exd.12336