Search results for "CELLULAR"
showing 10 items of 6449 documents
Computing temporal sequences associated with dynamic patterns on the C. elegans connectome
2021
AbstractUnderstanding how the structural connectivity of a network constrains the dynamics it is able to support is a very active and open area of research. We simulated the plausible dynamics resulting from the known C. elegans connectome using a recent model and theoretical analysis that computes the dynamics of neurobiological networks by focusing on how local interactions among connected neurons give rise to the global dynamics in an emergent way, independent of the biophysical or molecular details of the cells themselves. We studied the dynamics which resulted from stimulating a chemosensory neuron (ASEL) in a known feeding circuit, both in isolation and embedded in the full connectome…
A ceRNA analysis on LMNA gene focusing on the Hutchinson-Gilford progeria syndrome
2013
Background: Hutchinson-Gilford progeria syndrome is a rare dominant human disease of genetic origin. The average life expectancy is about 20 years, patients’ life quality is still very poor and no efficient therapy has yet been developed. It is caused by mutation of the LMNA gene, which results in accumulation in the nuclear membrane of a particular splicing form of Lamin-A called progerin. The mechanism by which progerin perturbs cellular homeostasis and leads to the symptoms is still under debate. Micro-RNAs are able to negatively regulate transcription by coupling with the 3’ UnTranslated Region of messenger RNAs. Several Micro-RNAs recognize the same 3’ UnTranslated Region and each Micr…
Sp1 transcription factor interaction with accumulated prelamin a impairs adipose lineage differentiation in human mesenchymal stem cells: essential r…
2012
Abstract Lamin A (LMNA)-linked lipodystrophies may be either genetic (associated with LMNA mutations) or acquired (associated with the use of human immunodeficiency virus protease inhibitors [PIs]), and in both cases they share clinical features such as anomalous distribution of body fat or generalized loss of adipose tissue, metabolic alterations, and early cardiovascular complications. Both LMNA-linked lipodystrophies are characterized by the accumulation of the lamin A precursor prelamin A. The pathological mechanism by which prelamin A accumulation induces the lipodystrophy associated phenotypes remains unclear. Since the affected tissues in these disorders are of mesenchymal origin, we…
ANKRD26-RET - A novel gene fusion involving RET in papillary thyroid carcinoma
2018
Abstract Background Rearrangements of RET are drivers of oncogenesis, traceable in different cancer types as papillary thyroid carcinoma (PTC), non-small cell lung cancer, colorectal or breast cancer. Anchored multiplex PCR based next-generation sequencing (NGS) can detect RET rearrangements involving previously unknown partner genes. Methods A sample of PTC underwent NGS, following detection of RET rearrangement by fluorescence in situ hybridization. Expression analysis of ANKRD26 and RET was performed for the tumor harboring ANKRD26-RET, for corresponding normal thyroid tissue and PTC tumors with representative genetic alterations (BRAFV600E, CCDC6-RET), complemented by a comparative sear…
Cortical gene expression in spinal cord injury and repair: insight into the functional complexity of the neural regeneration program
2011
Traumatic spinal cord injury (SCI) results in the formation of a fibrous scar acting as a growth barrier for regenerating axons at the lesion site. We have previously shown (Klapka et al., 2005) that transient suppression of the inhibitory lesion scar in rat spinal cord leads to long distance axon regeneration, retrograde rescue of axotomized cortical motoneurons, and improvement of locomotor function. Here we applied a systemic approach to investigate for the first time specific and dynamic alterations in the cortical gene expression profile following both thoracic SCI and regeneration-promoting anti-scarring treatment (AST). In order to monitor cortical gene expression we carried out micr…
The case for simplifying and using absolute targets for viral hepatitis elimination goals
2021
The 69th World Health Assembly endorsed the Global Health Sector Strategy for Viral Hepatitis, embracing a goal to eliminate hepatitis infection as a public health threat by 2030. This was followed by the World Health Organization's (WHO) global targets for the care and management of hepatitis B virus (HBV) and hepatitis C virus (HCV) infections. These announcements and targets were important in raising awareness and calling for action; however, tracking countries’ progress towards these elimination goals has provided insights to the limitations of these targets. The existing targets compare a country's progress relative to its 2015 values, penalizing countries who started their programmes …
Sobre substitució lingüística i autòmats cel.lulars (SLAC): decurs de la investigació
2010
Hem optat per focalitzar la nostra investigació, tot i l'ampla aplicabilitat de la recerca, en la detecció del futur de la nostra llengua a partir dels pressupòsits de la Psicologia Social Computacional. I això mitjançant la utilització de la simulació informàtica, basada en els autòmats cel·lulars, que ha donat els primers resultats que presentem i explicitem en la nostra aportació a l'ANUARI. Així, hi abordem el futur de la llengua pròpia a partir de la conducta referida als parlants del País Valencià i amb la intenció d'esbrinar la reversió de la substitució lingüística.
Cuantificación de la calidad embrionaria mediante el consumo de oxígeno durante el proceso rutinario de incubación y desarrollo in vitro
2015
A pesar de la aplicación intensiva de las TRA y de las cada vez mayores tasas de éxito, hay ciertos aspectos de estos tratamientos que continúan sin resolverse, siendo una limitación importante el hecho de que sólo entre el 10-20% de los embriones transferidos al útero sean capaces de dar lugar a un embarazo a término. Esta limitación está promoviendo el desarrollo de nuevas técnicas no invasivas para seleccionar embriones más eficazmente que mediante el mero criterio morfológico. Las evidencias apoyadas por distintos trabajos encontrados en la literatura hacen que consideremos algunos métodos no invasivos, como la actividad metabólica de ovocitos y embriones, como buenos predictores de la …
Mobile addiction e prevenzione attraverso il gruppo dei pari
2014
La dipendenza da cellulare, espressione dei profondi cambiamenti economici, sociali e culturali degli ultimi decenni, sta diventando un problema sempre piu diffuso, soprattutto fra gli adolescenti. L’articolo, nella prima parte si sofferma sull’analisi della letteratura relativa alla dipendenza tecnologica, in generale, e alla dipendenza da cellulare, nello specifico; nella seconda parte, presenta una ricerca-intervento che, da un lato, ha avuto l’obiettivo di esaminare la relazione tra fattori cognitivi (locus of control) e fattori emotivi (disregolazione emotiva) nei casi di abuso nell’utilizzo di telefoni cellulari; dall’altro, ha voluto avviare una riflessione circa l’influenza del grup…
Examinations of Bilateral Epileptiform Activities in Hippocampal Slices Obtained From Young Mice
2021
Bilateral interconnections through the hippocampal commissure play important roles in synchronizing or spreading hippocampal seizure activities. Intact hippocampi or bilateral hippocampal slices have been isolated from neonatal or immature rats (6–7 or 12–21 days old, respectively) and the mechanisms underlying the bilateral synchrony of hippocampal epileptiform activities have been investigated. However, the feasibility of examining bilateral epileptiform activities of more developed hippocampal circuitryin vitroremains to be explored. For this, we prepared bilateral hippocampal slices from C57 black mice, a strain commonly used in neuroscience and for genetic/molecular modifications. Youn…