Search results for "CENTRIFUGE"
showing 10 items of 30 documents
Distribution of chlorpromazine in a simplified blood influenced by various drugs
1973
The binding of chlorpromazine to erythrocytes and to albumin as influenced by other drugs was studied in a simplified blood (31.5±0.3% bovine erythrocytes, 4 g-% bovine serum albumin in 0.02 M phosphate buffer solution containing 0.15 M NaCl). the total concentration of chlorpromazine in the simplified blood was 10−4 M, the concentration of the displacing drugs was 10−3 M. After an incubation period of 3 h at 22° C the chlorpromazine concentration was determined in the albumin solution after centrifugation of the blood at 3000×g and in the aqueous phase after ultracentrifugation at 150000×g. Under control conditions 68.1±0.9% of chlorpromazine was bound to the erythrocytes, 28.5±0.9% was bo…
Native and subunit molecular mass and quarternary structure of the hemoglobin from the primitive branchiopod crustacean Triops cancriformis
2006
Many branchiopod crustaceans are endowed with extracellular, high-molecular-weight hemoglobins whose exact structural characteristics have remained a matter of conjecture. By using a broad spectrum of techniques, we provide precise and coherent information on the hemoglobin of one of the phylogenetically ‘oldest’ extant branchiopods, the tadpole shrimp Triops cancriformis. The hemoglobin dissociated under reducing conditions into two subunits, designated TcHbA and TcHbB, with masses of 35 775 ± 4 and 36 055 ± 4 Da, respectively, determined by ESI-MS. Nonreducing conditions showed only two disulfide-bridged dimers, a homodimer of TcHbA, designated D1 (71 548 ± 5 Da), and the heterodimer D2 (…
Rotor—frame contact in a centrifuge installed on board a ship
2009
The criticality of rotor and stator contact in a large-size marine separator is studied by numerical modelling. Two plausible mechanisms leading to such contact, namely rolling of a ship in steady-state motion and a suddenly occurring unbalance in the rotor, are considered. It is found that contact due to sea motion may be tolerable, while sudden development of unbalance at operating speed can lead to unacceptably high contact forces. This agrees with experience of centrifugal separators operating on board of ships.
Continuous separation and identification of neutronrich neptunium isotopes from heavy-ion reactions by means of the centrifuge system “sisak”
1986
Abstract Neutron-rich neptunium isotopes formed in direct transfer reactions between 136 Xe projectiles and targets of 244 Pu have been separated from other reaction products with the on-line liquid-liquid extraction system SISAK. A four-detector delay method was used to determine the half-lives of the previously unknown isotopes 243 Np and 244 Np. From the decay of the 287.4 keV γ-line a half-life of 1.8 ± 0.3 min was determined for 243 Np. From the decay curves of the γ-lines at 162.7 and 216.7 keV, which fit into the known level scheme of 244 Pu, a half-life of 2.5 ± 0.3 min results for 244 Np.
Controlling ground-state rotational dynamics of molecules by shaped femtosecond laser pulses
2004
We report controlled excitation of ground-state rotational wave packet by pulse-shaping technique. The experiment is conducted in nitrogen $({\mathrm{N}}_{2})$ at room temperature and atmospheric pressure. A femtosecond laser pulse produces rotational coherences in the vibronic ground state of ${\mathrm{N}}_{2}$ through an impulsive Raman process. The laser pulse is tailored using a spatial light modulator producing spectral phase modulation. Periodic phase steps are applied in order to control the excitation of specific rotational Raman transitions. The outcome is the modification of the relative excitation between odd and even rotational states which allows the control of the symmetry and…
A Classical Synthesis of the Collagen-like Peptides with the Sequence Z(GlyProPro)nOBut and their characterization with circular dichroism and ultrac…
1975
Oligopeptides with the sequence Z(GlyProPro)nOBut (n = 3–7) were synthesized along a clasical pathway. Generally long fragments were condensed in order to allow an easy separation of the products from the reactants. It is established by molecular weight determination and measurements of circular dichroism that the peptides form the collagen-like triple helix in methanol and that they assume the random coil conformation in dilute acetic acid. The circular dichroism spectra agree reasonably well with the corresponding spectra for (ProProGly)10 which was obtained by solid phase synthesis in another laboratory.
Two-step cross correlation-based algorithm for motion estimation applied to fertilizer granules' motion during centrifugal spreading
2011
Imaging systems are progressing in both accuracy and ro- bustness, and their use in precision agriculture is increasing accordingly. One application of imaging systems is to understand and control the cen- trifugal fertilizing spreading process. Predicting the spreading pattern on the ground relies on an estimation of the trajectories and velocities of ejected granules. The algorithms proposed to date have shown low ac- curacy, with an error rate of a few pixels. But a more accurate estimation of the motion of the granules can be achieved. Our new two-step cross- correlation-based algorithm is based on the technique used in particle image velocimetry (PIV), which has yielded highly accurate…
Physicochemical characterization of the fifth (C5), sixth (C6), seventh (C7), eighth (C8) and ninth (C9) component of guinea pig complement.
1971
A physicochemical characterization of the purified guinea pig complement components C5 to C9 is given. For this purpose the sedimentation rate, the diffusion coefficient, the molecular weight and the isoelectric point were determined and compared with the values already known for the guinea pig and human complement system. For the determination of the physicochemical parameters gel filtration on Sephadex G-200, ultracentrifugation applying a sucrose density gradient and thin-layer isoelectric focusing were used. By comparing the values of the human and guinea pig complement a remarkable similarity is shown.
Rapid Radiochemical Separation Procedures from Aqueous Solutions
1992
The state-of-the-art in the field of rapid radiochemical separation procedures is illustrated by two systems using aqueous solutions as input: ARCA, an apparatus for repetitive separations, and SISAK, a centrifuge system for continuous separations. Applications presented are: Solvent extraction of element 105 investigated with 34-s 262105 using ARCA, and decay scheme studies of the fission product 1.0-s 110Tc by means of SISAK.
The molecular heterogeneity of hemocyanin: Structural and functional properties of the 4×6-meric protein of Upogebia pusilla (Crustacea)
2006
The structural properties of the hemocyanin isolated from the Mediterranean mud shrimp, Upogebia pusilla (Decapoda: Thalassinidea), were investigated. Our intent was to make use of the U. pusilla case to perform a structural comparison between crustacean and chelicerate 4×6-meric hemocyanins. The thalassinidean hemocyanin appears similar in size but different in structural organization compared to the chelicerate 4×6-mer. Ultracentrifuge analyses on the purified protein revealed a sedimentation coefficient of 39S, typical of 4×6 hemocyanins. Electron micrographs are in agreement with a model in which four 2×6-meric building blocks are arranged in a tetrahedron-like quaternary structure and …