6533b839fe1ef96bd12a6603
RESEARCH PRODUCT
Physicochemical characterization of the fifth (C5), sixth (C6), seventh (C7), eighth (C8) and ninth (C9) component of guinea pig complement.
Manfred P. DierichDieter Bitter-suermannUlrich HaddingW. Königsubject
ErythrocytesDensity gradientChemical PhenomenaImmunologySize-exclusion chromatographyGuinea PigsBiologyGuinea pigHemoglobinsCentrifugation Density GradientImmunology and AllergyAnimalsHumansChromatographyIsoelectric focusingChemistry PhysicalVenomsElectric ConductivitySnakesComplement System ProteinsCatalaseComplement systemMolecular WeightIsoelectric pointSephadexImmunoglobulin GImmunologyChromatography GelUltracentrifugeIsoelectric Focusingdescription
A physicochemical characterization of the purified guinea pig complement components C5 to C9 is given. For this purpose the sedimentation rate, the diffusion coefficient, the molecular weight and the isoelectric point were determined and compared with the values already known for the guinea pig and human complement system. For the determination of the physicochemical parameters gel filtration on Sephadex G-200, ultracentrifugation applying a sucrose density gradient and thin-layer isoelectric focusing were used. By comparing the values of the human and guinea pig complement a remarkable similarity is shown.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1971-11-01 | European journal of immunology |