Search results for "CET"
showing 9 items of 6679 documents
Investor-state dispute settlement - podstępne zagrożenie dla sprawnego działania administracji publicznej
2017
ISDS (investor-state dispute settlement, znany także jako ICS - investment court system) to umowny mechanizm rozstrzygania sporów między inwestorem a państwem. Polega on na możliwości zaskarżenia państwa przez przedsiębiorcę (inwestora) do arbitrażu wówczas, gdy państwo wydaje lub wykorzystuje przepisy ograniczające zyski przedsiębiorstwa (praktyki dyskryminujące). Arbitrami ISDS jest kilkunastu sowicie opłacanych prawników, pracujących dla wielkich kancelarii międzynarodowych, którzy niemal nigdy nie orzekają na korzyść państwa. Przedsiębiorca może żądać wysokiego odszkodowania: nie tylko zwrotu utraconych korzyści, lecz także spodziewanych zysków, których wysokości nie musi nawet drobiazg…
Unità e paradosso. Un événement de l’âme?
2020
La sura 112 inizia con il pronome huwa (egli) e rivela l'unità assoluta e inaccessibile di Dio. Il tawḥīd è l'atto di fede fondato sulla rivelazione del pronome dell'assente e rischia di essere un monoteismo paradossale. Per il trinitarismo la relazione è sostanziale, esclusa dal monoteismo assoluto islamico che per superare il paradosso necessita della teofania docetica. Henri Corbin si rivolge a questa dottrina extra-ipostatica e fonda la mediazione Dio-uomo esclusivamente sullʼascesa dellʼuomo mediante uccessive teofanie. Questa antropologia è unʼimamologia modellata sulla cristologia angelomorfica e nega la salvezza mediante la croce. La radice del docetismo è divelta dallʼunione iposta…
Modulation of Hepatitis C Virus NS5A Hyperphosphorylation by Nonstructural Proteins NS3, NS4A, and NS4B
1999
NS5A of the hepatitis C virus (HCV) is a highly phosphorylated protein involved in resistance against interferon and required most likely for replication of the viral genome. Phosphorylation of this protein is mediated by a cellular kinase(s) generating multiple proteins with different electrophoretic mobilities. In the case of the genotype 1b isolate HCV-J, in addition to the basal phosphorylated NS5A (designated pp56), a hyperphosphorylated form (pp58) was found on coexpression of NS4A (T. Kaneko, Y. Tanji, S. Satoh, M. Hijikata, S. Asabe, K. Kimura, and K. Shimotohno, Biochem. Biophys. Res. Commun. 205:320‐326, 1994). Using a comparative analysis of two full-length genomes of genotype 1b…
Translocation of the nuclear autoantigen La to the cell surface of herpes simplex virus type 1 infected cells.
1992
Recently we developed a procedure to translocalize one of the extractable nuclear antigens (ENAs), the La protein, to the cell surface of CV-1 cells. Here we report that herpes simplex virus type 1 infection can also induce a translocation of the autoantigen to the cell surface. On the cell surface we detected La protein assembled with large protrusions. Within these protrusions La protein colocalized with virus particles. These protrusions are known to be released from the cell after virus infections. Such complexes consisting of self and virus could provide helper determinants for an anti-self response, and therefore be important in generation of autoimmunity.
In vitro studies on the activation of the hepatitis C virus NS3 proteinase by the NS4A cofactor.
1996
AbstractProteolytic processing of the nonstructural proteins of the hepatitis C virus (HCV) is mediated by two viral proteinases: the NS2-3 proteinase cleaving at the NS2/3 junction and the NS3 serine-type proteinase responsible for processing at the NS3/4A, NS4A/B, NS4B/5A, and NS5A/B sites. Activity of the NS3 proteinase is modulated by NS4A. In the absence of this cofactor processing at the NS3-dependent sites does not occur or, in the case of the NS5A/B junction, is poor but increased when NS4A is present. Although recent studies demonstrated that proteinase activation requires direct interaction between NS3 and NS4A, the mechanism by which NS4A exerts the activation function is not kno…
Single amino acid substitutions in the glycoprotein B carboxy terminus influence the fusion from without property of herpes simplex virus type 1.
1995
Syncytial mutations of herpes simplex virus type 1 (HSV-1) strains ANG, ANG path, HFEM, tsB5 and HSZP cause extensive cell fusion and were mapped to the cytoplasmic domain of glycoprotein B (gB), within the syn 3 locus. These strains are so far the only ones which show the phenotype ‘fusion from without’ (FFWO): 60 min after infection with high m.o.i., cells in a tissue culture are fused without transcription and translation of the viral genome. In this report we detected, using the recombinants 27/III and K-7, that an amino acid exchange from Ala to Val at aa position 854 of gB is the main determinant for FFWO activity of strains ANG, ANG path and recombinant K-7. The transfer of this muta…
CCDC 1917751: Experimental Crystal Structure Determination
2019
Related Article: Víctor García-López, Mario Palacios-Corella, Verónica Gironés-Pérez, Carlos Bartual-Murgui, José Antonio Real, Eric Pellegrin, Javier Herrero-Martín, Guillem Aromí, Miguel Clemente-León, Eugenio Coronado|2019|Inorg.Chem.|58|12199|doi:10.1021/acs.inorgchem.9b01526
Antecedentes, consecuencias y paradojas del Brexit
2018
En el referéndum celebrado en junio del 2016, los votantes británicos optaron por abandonar la Unión Europea. Este artículo revisa las consecuencias económicas y los antecedentes del Brexit. La evidencia que arrojan los estudios realizados hasta la fecha sugiere unos efectos negativos del Brexit. El diseño de la votación, el descontento con la crisis y los efectos de la globalización ayudan a explicar la paradoja según la cual los votantes optaron por una opción en contra de sus propios intereses económicos. La modulación de estos efectos dependerá del acuerdo final que se negocie entre la UE y el Reino Unido. Sin embargo, existen dos cuestiones abiertas en relación con el Brexit. En primer…
α-Conotoxins EpI and AuIB switch subtype selectivity and activity in native versus recombinant nicotinic acetylcholine receptors
2003
The Xenopus laevis oocyte expression system was used to determine the activities of alpha-conotoxins EpI and the ribbon isomer of AuIB, on defined nicotinic acetylcholine receptors (nAChRs). In contrast to previous findings on intracardiac ganglion neurones, alpha-EpI showed no significant activity on oocyte-expressed alpha3beta4 and alpha3beta2 nAChRs but blocked the alpha7 nAChR with an IC50 value of 30 nM. A similar IC50 value (103 nM) was obtained on the alpha7/5HT3 chimeric receptor stably expressed in mammalian cells. Ribbon AuIB maintained its selectivity on oocyte-expressed alpha3beta4 receptors but unlike in native cells, where it was 10-fold more potent than native alpha-AuIB, had…