Search results for "CG"

showing 10 items of 999 documents

Human norovirus hyper-mutation revealed by ultra-deep sequencing

2016

Human noroviruses (NoVs) are a major cause of gastroenteritis worldwide. It is thought that, similar to other RNA viruses, high mutation rates allow NoVs to evolve fast and to undergo rapid immune escape at the population level. However, the rate and spectrum of spontaneous mutations of human NoVs have not been quantified previously. Here, we analyzed the intra-patient diversity of the NoV capsid by carrying out RT-PCR and ultra-deep sequencing with 100,000-fold coverage of 16 stool samples from symptomatic patients. This revealed the presence of low-frequency sequences carrying large numbers of U-to-C or A-to-G base transitions, suggesting a role for hyper-mutation in NoV diversity. To mor…

0301 basic medicineMutation rateVirologiaGene ExpressionVirus Replicationmedicine.disease_causeFecesMutation RateHuman genetics[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseasesCloning MolecularComputingMilieux_MISCELLANEOUSCaliciviridae InfectionsGeneticsMutation[SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseasesGenètica humanaHigh-Throughput Nucleotide SequencingGastroenteritisInfectious Diseases[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/VirologyRNA ViralHyper-mutationMicrobiology (medical)RNA virus[SDE.MCG]Environmental Sciences/Global ChangesContext (language use)BiologyTransfectionMicrobiologyArticleDNA sequencingViral Proteins03 medical and health sciences[SDV.EE.ECO]Life Sciences [q-bio]/Ecology environment/EcosystemsVirologyGeneticsmedicineHumansMolecular BiologyGeneEcology Evolution Behavior and Systematics[SDV.EE.SANT]Life Sciences [q-bio]/Ecology environment/HealthBase SequenceNorovirusRNA virusbiology.organism_classificationVirology[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/BacteriologyHEK293 Cells030104 developmental biologyViral replicationNext-generation sequencingNorovirus[SDE.BE]Environmental Sciences/Biodiversity and Ecology
researchProduct

Weakly coupled map lattice models for multicellular patterning and collective normalization of abnormal single-cell states

2017

We present a weakly coupled map lattice model for patterning that explores the effects exerted by weakening the local dynamic rules on model biological and artificial networks composed of two-state building blocks (cells). To this end, we use two cellular automata models based on: (i) a smooth majority rule (model I) and (ii) a set of rules similar to those of Conway's Game of Life (model II). The normal and abnormal cell states evolve according with local rules that are modulated by a parameter $\kappa$. This parameter quantifies the effective weakening of the prescribed rules due to the limited coupling of each cell to its neighborhood and can be experimentally controlled by appropriate e…

0301 basic medicineNormalization (statistics)Majority ruleTime FactorsFOS: Physical sciencesAbnormal cellPattern Formation and Solitons (nlin.PS)Models BiologicalCell Physiological PhenomenaCombinatorics03 medical and health sciences0302 clinical medicineCell Behavior (q-bio.CB)Physics - Biological PhysicsGame of lifeMathematicsCellular Automata and Lattice Gases (nlin.CG)Artificial networksNonlinear Sciences - Pattern Formation and SolitonsCellular automatonMulticellular organism030104 developmental biologyBiological Physics (physics.bio-ph)030220 oncology & carcinogenesisFOS: Biological sciencesQuantitative Biology - Cell BehaviorBiological systemNonlinear Sciences - Cellular Automata and Lattice GasesCoupled map lattice
researchProduct

Jacobsen syndrome and neonatal bleeding: report on two unrelated patients

2021

Abstract Introduction In 1973, Petrea Jacobsen described the first patient showing dysmorphic features, developmental delay and congenital heart disease (atrial and ventricular septal defect) associated to a 11q deletion, inherited from the father. Since then, more than 200 patients have been reported, and the chromosomal critical region responsible for this contiguous gene disorder has been identified. Patients’ presentation We report on two unrelated newborns observed in Italy affected by Jacobsen syndrome (JBS, also known as 11q23 deletion). Both patients presented prenatal and postnatal bleeding, growth and developmental delay, craniofacial dysmorphisms, multiple congenital anomalies, a…

0301 basic medicinePediatricsmedicine.medical_specialtyGenotype-phenotype correlationHeart diseaseGenetic counselingCase ReportIn situ hybridization030105 genetics & heredityPediatricsRJ1-57003 medical and health sciences0302 clinical medicineaCGHJBSmedicineHumansJacobsen Distal 11q Deletion SyndromeJacobsen syndromeCraniofacialGenetic Association StudiesCerebral Hemorrhage11q23 deletionbusiness.industryInfant NewbornEarly diagnosimedicine.diseaseEarly diagnosisPancytopeniaThrombocytopeniaItalyFemalePresentation (obstetrics)business030217 neurology & neurosurgeryComparative genomic hybridizationItalian Journal of Pediatrics
researchProduct

Mechanisms of astringency: Structural alteration of the oral mucosal pellicle by dietary tannins and protective effect of bPRPs

2018

International audience; The interaction of tannins with salivary proteins is involved in astringency. This paper focussed on saliva liningoral mucosae, the mucosal pellicle. Using a cell-based model, the impact of two dietary tannins (EgC and EgCG)on the mucosal pellicle structure and properties was investigated by microscopic techniques. The role of basicProline-Rich-Proteins (bPRPs) in protecting the mucosal pellicle was also evaluated.At low (0.05 mM) tannin concentration, below the sensory detection threshold, the distribution of salivarymucins MUC5B on cells remained unaffected. At 0.5 and 1 mM, MUC5B-tannin aggregates were observed andtheir size increased with tannin concentration and…

0301 basic medicineSalivaFrictionAstringencyMicroscopy Atomic ForceCatechinCell LineAnalytical ChemistryProtein Aggregates03 medical and health sciences0404 agricultural biotechnologyHumansTanninDental PellicleFood scienceSalivaAstringentsEgCGchemistry.chemical_classificationR146/MUC1 cells030109 nutrition & dietetics[PHYS.PHYS]Physics [physics]/Physics [physics]ChemistryAtomic force microscopyDetection thresholdSalivary mucins MUC5BMucinMouth Mucosa04 agricultural and veterinary sciencesGeneral MedicineMucin-5B040401 food scienceDietSalivary Proline-Rich ProteinsAtomic Force MicroscopyOn cellsMicroscopy Electron ScanningSalivary ProteinsIB5Scanning Electron MicroscopyTannins[SDV.AEN]Life Sciences [q-bio]/Food and NutritionFood Science
researchProduct

Harnessing Unconventional T Cells for Immunotherapy of Tuberculosis

2020

Even if the incidence of tuberculosis (TB) has been decreasing over the last years, the number of patients with TB is increasing worldwide. The emergence of multidrug-resistant and extensively drug-resistant TB is making control of TB more difficult. Mycobacterium bovis bacillus Calmette–Guérin vaccine fails to prevent pulmonary TB in adults, and there is an urgent need for a vaccine that is also effective in patients with human immunodeficiency virus (HIV) coinfection. Therefore, TB control may benefit on novel therapeutic options beyond antimicrobial treatment. Host-directed immunotherapies could offer therapeutic strategies for patients with drug-resistant TB or with HIV and TB coinfecti…

0301 basic medicinelcsh:Immunologic diseases. AllergyTuberculosismedicine.medical_treatmentT cellImmunologyCD1HIV InfectionsMajor histocompatibility complexMucosal-Associated Invariant T Cellshost-directed therapy03 medical and health sciences0302 clinical medicineMHC class ImedicineImmunology and AllergyHumansTuberculosis Pulmonarybiologybusiness.industryImmunotherapyMycobacterium tuberculosisDonor Lymphocytesmedicine.diseaseAdoptive Transfer030104 developmental biologymedicine.anatomical_structuretuberculosisImmunologybiology.proteinCoinfectionBCG VaccineHIV-1cytotoxicityT cell receptorbusinesslcsh:RC581-607unconventional T cells030215 immunologyFrontiers in Immunology
researchProduct

Prevalence of Listeria spp. and characterization of Listeria monocytogenes isolated from food products in Tetouan, Morocco

2018

A total of 1096 food samples were purchased in Tetouan, North-Western of Morocco, to examine the presence of Listeria spp. Eighty (7.3%) of the tested samples were found positive for the presence of Listeria spp., while L. monocytogenes was detected in 16 (1.5%) samples. L. monocytogenes isolates belonged to clonal complexes CC2 (PCR serogroup IVb) and CC199 (PCR serogroup Ila) and to 8 different combined AscI/Apal pulsed-field gel electrophoresis profiles. Core genome multilocus sequence typing (cgMLST) allowed to distinguish 12 different cgMLST types and revealed the presence of 3 clusters of closely-related isolates from different samples, suggesting the existence of common sources of co…

0301 basic medicinemedicine.drug_classListeriaPCR-Serogrouping030106 microbiologyAntibioticsPrevalenceBiologymedicine.disease_causeMicrobiology03 medical and health sciencesListeria monocytogenes[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseasesmedicineComputingMilieux_MISCELLANEOUS2. Zero hungerGel electrophoresisbusiness.industryPFGEbiology.organism_classificationFood safety[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/BacteriologyMorocco030104 developmental biology[SDV.MP]Life Sciences [q-bio]/Microbiology and ParasitologyFood productsListeriaMultilocus sequence typingcgMLSTbusiness[SDV.MHEP]Life Sciences [q-bio]/Human health and pathologyFood ScienceBiotechnology
researchProduct

Blocking CGRP in migraine patients – a review of pros and cons

2017

Abstract Migraine is the most prevalent neurological disorder worldwide and it has immense socioeconomic impact. Currently, preventative treatment options for migraine include drugs developed for diseases other than migraine such as hypertension, depression and epilepsy. During the last decade, however, blocking calcitonin gene-related peptide (CGRP) has emerged as a possible mechanism for prevention of migraine attacks. CGRP has been shown to be released during migraine attacks and it may play a causative role in induction of migraine attacks. Here, we review the pros and cons of blocking CGRP in migraine patients. To date, two different classes of drugs blocking CGRP have been developed: …

0301 basic medicinemedicine.medical_specialtyanesthesiology and pain medicineNeurologyCalcitonin Gene-Related PeptideMigraine DisordersCentral nervous systemlcsh:MedicineNeurological disorderReview ArticleCalcitonin gene-related peptidePlaceboBioinformatics03 medical and health sciencesEpilepsy0302 clinical medicineCalcitonin Gene-Related Peptide Receptor AntagonistsMedicineAnimalsHumansMigraineneurology (clinical)integumentary systembusiness.industrylcsh:Racute treatment; CGRP; CGRP receptor; gepants; migraine; prophylactic treatment; neurology (clinical); anesthesiology and pain medicineCgrp receptorGeneral MedicineGepantsmedicine.disease3. Good health030104 developmental biologymedicine.anatomical_structureMigrainenervous systemAnesthesiaAcute treatment; Cgrp; Cgrp receptor; Gepants; Migraine; Prophylactic treatment; Neurology (clinical); Anesthesiology and Pain MedicineCgrpAnimal studiesbusinessGepantProphylactic treatmentAcute treatment030217 neurology & neurosurgery
researchProduct

Liver X Receptor–Mediated Induction of Cholesteryl Ester Transfer Protein Expression Is Selectively Impaired in Inflammatory Macrophages

2009

Objective— Cholesteryl ester transfer protein (CETP) is a target gene for the liver X receptor (LXR). The aim of this study was to further explore this regulation in the monocyte-macrophage lineage and its modulation by lipid loading and inflammation, which are key steps in the process of atherogenesis. Methods and Results— Exposure of bone marrow–derived macrophages from human CETP transgenic mice to the T0901317 LXR agonist increased CETP, PLTP, and ABCA1 mRNA levels. T0901317 also markedly increased CETP mRNA levels and CETP production in human differentiated macrophages, whereas it had no effect on CETP expression in human peripheral blood monocytes. In inflammatory mouse and human mac…

030204 cardiovascular system & hematologyMonocytesMice0302 clinical medicinepolycyclic compoundsPhospholipid Transfer ProteinsCells CulturedLiver X Receptors0303 health sciencesCell DifferentiationOrphan Nuclear ReceptorsUp-RegulationLipoproteins LDLmedicine.anatomical_structureABCG1Models Animalmonocytelipids (amino acids peptides and proteins)medicine.symptomCardiology and Cardiovascular MedicineOxidation-ReductionAgonistmedicine.medical_specialtymedicine.drug_classBlotting Westerncholesteryl ester transfer proteinMice TransgenicInflammationmacrophageBiology03 medical and health sciencesDownregulation and upregulationInternal medicineCholesterylester transfer proteinmedicineAnimalsHumans[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular BiologyRNA MessengerLiver X receptorLiver X receptorProbability030304 developmental biologyMacrophagesMonocyteAtherosclerosisCholesterol Ester Transfer Proteinscarbohydrates (lipids)EndocrinologyGene Expression RegulationinflammationABCA1Immunologybiology.protein[SDV.AEN]Life Sciences [q-bio]/Food and NutritionArteriosclerosis, Thrombosis, and Vascular Biology
researchProduct

Entre éducation populaire et propagande syndicale : les cours radiophoniques de la CGT sous le Front Populaire

2011

Notre article se propose d’aborder une expérience originale d’éducation populaire, souvent citée mais finalement assez peu étudiée : les cours radiodiffusés du Centre confédéral d’éducation ouvrière de la CGT, durant le Front populaire. Le CCEO qui, dès sa création en 1933, s’est voulu un outil de démocratisation du savoir va en effet avoir l’opportunité d’accéder à un média de masse à partir de janvier 1937 et d’élargir son audience. Nous verrons donc comment pendant trois ans, à travers l’émission La Voix de la CGT, le syndicat a investi les ondes, fait l’apprentissage des exigences particulières de l’exercice radiophonique et s’est efforcé de concilier mission éducative et tentation prop…

0303 health sciencesHistoryCCEOSociology and Political ScienceCGTEducation populaire[ SHS.HIST ] Humanities and Social Sciences/History4. EducationCultureFormation syndicaleRadio03 medical and health sciences0302 clinical medicine030220 oncology & carcinogenesis[SHS.HIST] Humanities and Social Sciences/HistorySyndicalismeFront populaire[SHS.HIST]Humanities and Social Sciences/History030304 developmental biology
researchProduct

"Exclusion contour 2(exp.)" of "Search for squarks and gluinos in final states with hadronically decaying $\tau$-leptons, jets, and missing transvers…

2018

Expected exclusion contour at 95% CL as a function of tanBeta and the SUSY-breaking mass scale Lambda.

13000.0CLSGMSB M(MESS) = 250 TEV N5 = 3 MU > 0 CGRAV = 1
researchProduct