Search results for "CHIME"
showing 10 items of 124 documents
Tracing the first steps of American sturgeon pioneers in Europe
2008
Abstract Background A Baltic population of Atlantic sturgeon was founded ~1,200 years ago by migrants from North America, but after centuries of persistence, the population was extirpated in the 1960s, mainly as a result of over-harvest and habitat alterations. As there are four genetically distinct groups of Atlantic sturgeon inhabiting North American rivers today, we investigated the genetic provenance of the historic Baltic population by ancient DNA analyses using mitochondrial and nuclear markers. Results The phylogeographic signal obtained from multilocus microsatellite DNA genotypes and mitochondrial DNA control region haplotypes, when compared to existing baseline datasets from extan…
Novel antiproliferative chimeric compounds with marked histone deacetylase inhibitory activity.
2014
Given our interest in finding potential antitumor agents and in view of the multifactorial mechanistic nature of cancer, in the present work, taking advantage of the multifunctional ligands approach, new chimeric molecules were designed and synthesized by combining in single chemical entities structural features of SAHA, targeting histone deacetylases (HDACs), with substituted stilbene or terphenyl derivatives previously obtained by us and endowed with antiproliferative and pro-apoptotic activity. The new chimeric derivatives were characterized with respect to their cytotoxic activity and their effects on cell cycle progression on different tumor cell lines, as well as their HDACs inhibitio…
Virally Infected Mouse Liver Endothelial Cells Trigger CD8+ T-Cell Immunity
2009
Background & Aims Dendritic cell activation through ligation of pattern recognition receptors leading to full functional maturation causes induction of CD8 + T-cell immunity through increased delivery of costimulatory signals instead of tolerance. Here we investigate whether organ-resident antigen-presenting cells, such as liver sinusoidal endothelial cells (LSECs), also switch from tolerogenic to immunogenic CD8 + T-cell activation upon such stimulation. Methods Murine LSECs were isolated by immunomagnetic separation and analyzed for functional maturation upon triggering pattern recognition receptors or viral infection employing gene expression analysis and T cell coculture assays. In vivo…
CAR-T therapy in solid transplant recipients with post-transplant lymphoproliferative disease: case report and literature review
2021
Patients with postransplant lymphoproliferative disease (PTLD) who are refractory to rituximab-based regimens have extremely poor prognosis. Data is lacking in the setting of solid organ transplantation (SOT)-related PTLD treated with chimeric antigen receptor T-cell (CAR-T) therapy. Moreover, limited information is available on the influence of concomitant immunosuppressive drugs on CAR-T function. Here, we describe the clinical outcome in one PTLD patient and propose a strategy for tailoring immunosuppressive treatment and organ monitoring in patients with kidney allografts after CAR-T infusion. This report also reviews the limited published data in the setting of SOT-related PTLD treated…
Adoptive T cell therapy for the treatment of ovarian cancer
2015
Despite de advances in surgery and chemotherapy, the outcome of ovarian cancer patients has improved very little over the last 40 years. Given that ovarian cancer is an immunogenic tumor, immunotherapies offer a great promise as treatment of this dismal disease. We show that follicle-stimulating hormone (FSH) receptor can be a very specific target that is expressed in >56% of human ovarian carcinomas and is a negative prognostic factor. Accordingly, we designed chimeric receptors expressing full-length FSH to redirect T-cell cytotoxic activity against these more aggressive tumors. In vivo, fully murine FSH-targeted T-cells and low affinity anti-mesothelin scFv-targeted Chimeric Antigen Rece…
Assessing Non-Photosynthetic Cropland Biomass from Spaceborne Hyperspectral Imagery
2021
Non-photosynthetic vegetation (NPV) biomass has been identified as a priority variable for upcoming spaceborne imaging spectroscopy missions, calling for a quantitative estimation of lignocellulosic plant material as opposed to the sole indication of surface coverage. Therefore, we propose a hybrid model for the retrieval of non-photosynthetic cropland biomass. The workflow included coupling the leaf optical model PROSPECT-PRO with the canopy reflectance model 4SAIL, which allowed us to simulate NPV biomass from carbon-based constituents (CBC) and leaf area index (LAI). PROSAIL-PRO provided a training database for a Gaussian process regression (GPR) algorithm, simulating a wide range of non…
Assembly of fluorescent chimeric virus-like particles of canine parvovirus in insect cells
2004
Canine parvovirus (CPV) is a small non-enveloped ssDNA virus composed of the viral proteins VP1, VP2, and VP3 with a T=1 icosahedral symmetry. VP2 is nested in VP1 and the two proteins are produced by differential splicing of a primary transcript of the right ORF of the viral genome. The VP2 protein can be further proteolytically cleaved to form VP3. Previous studies have shown that VP1 and VP3 are unnecessary for capsid formation and consequently, that VP2 alone is sufficient for assembly. We have hypothesized that insertion of the enhanced green fluorescent protein (EGFP) at the N-terminus of VP2 could be carried out without altering assembly. To investigate the possibility to develop flu…
V-Belt Winding along Archimedean Spirals During the Variator Speed Ratio Shift
2010
Starting from a previous model for the shift mechanics of rubber belt variators, this lecture elaborates practical design formulas for the torque and the axial thrust making use of the very close resemblance of the belt path to a linear spiral of Archimedes along a large part of the arc of contact. In addition, as an alternative to the modern calculus tools, it is shown how the drive variables can be equally calculated applying some propositions of Archimedes’ classical treatise πeρί ‘eλίκων (On Spirals).
SPARC oppositely regulates inflammation and fibrosis in bleomycin-induced lung damage.
2011
Fibrosis results from inflammatory tissue damage and impaired regeneration. In the context of bleomycin-induced pulmonary fibrosis, we demonstrated that the matricellular protein termed secreted protein acidic and rich in cysteine (SPARC) distinctly regulates inflammation and collagen deposition, depending on its cellular origin. Reciprocal Sparc(-/-) and wild-type (WT) bone marrow chimeras revealed that SPARC expression in host fibroblasts is required and sufficient to induce collagen fibrosis in a proper inflammatory environment. Accordingly, Sparc(-/-) >WT chimeras showed exacerbated inflammation and fibrosis due to the inability of Sparc(-/-) macrophages to down-regulate tumor necrosis …
Cellular automaton for chimera states
2016
A minimalistic model for chimera states is presented. The model is a cellular automaton (CA) which depends on only one adjustable parameter, the range of the nonlocal coupling, and is built from elementary cellular automata and the majority (voting) rule. This suggests the universality of chimera-like behavior from a new point of view: Already simple CA rules based on the majority rule exhibit this behavior. After a short transient, we find chimera states for arbitrary initial conditions, the system spontaneously splitting into stable domains separated by static boundaries, ones synchronously oscillating and the others incoherent. When the coupling range is local, nontrivial coherent struct…