Search results for "CLASSIFICATION"

El aprendizaje-servicio : una estrategia para la formación de competencias en sostenibilidad

2015

El artículo presenta la estrategia docente del Aprendizaje-Servicio como herramienta para desarrollar competencias vinculadas al reto de la sostenibilidad, tal y como establecen las directrices de la Conferencia de Rectores de las Universidades españolas (CRUE) y otras instancias institucionales a nivel local y global. Se analiza la experiencia docente de la autora, que ha utilizado la metodología del aprendizaje-servicio en la enseñanza universitaria para la formación de futuros y futuras profesionales de la educación en el logro de competencias clave exigidas por el desarrollo sostenible: el análisis crítico, la reflexión sistémica, la toma de decisión colaborativa, y el sentido de respon…

Automated approach for indirect immunofluorescence images classification based on unsupervised clustering method

2018

Autoimmune diseases (ADs) are a collection of many complex disorders of unknown aetiology resulting in immune responses to self-antigens and are thought to result from interactions between genetic and environmental factors. ADs collectively are amongst the most prevalent diseases in the U.S., affecting at least 7% of the population. The diagnosis of ADs is very complex, the standard screening methods provides seeking and recognizing of Antinuclear Antibodies (ANA) by Indirect ImmunoFluorescence (IIF) based on HEp-2 cells. In this paper an automatic system able to identify and classify the Centromere pattern is presented. The method is based on the grouping of centromeres present on the cell…

In vitro antitumor effects of the cold-water extracts of Mediterranean species of genus Pleurotus (higher Basidiomycetes) on human colon cancer cells

2014

The aim of this study was to evaluate whether the cold-water extracts of Pleurotus eryngii var. ferulae (CWE-Pef) and Pleurotus nebrodensis (CWE-Pn), 2 of the most prized wild and cultivated edible mushrooms, can affect the tumor phenotype of human colon cancer HCT116 cells. Our results showed that treatment with CWE- Pef and CWE-Pn resulted in a significant inhibition of the viability of HCT116 cells and promoted apoptosis, as also demonstrated by the increase of Bax-to-Bcl-2 messenger RNA ratio. Moreover, we observed that both extracts were able to inhibit cell migration and to affect homotypic and heterotypic cell-cell adhesion. It also was found that treatment with CWE-Pef and CWE-Pn ne…

Data from: The relevance of ecological status to ecosystem functions and services in a large boreal lake

2015

1. Environmental conventions aim to protect ecosystem structures and functions to provide goods and services for mankind. The degree of aquatic ecosystem naturalness, or ecological status as it is defined in the Water Framework Directive (WFD) of the European Union, is notionally linked to supplies of ecosystem services. In practice these links have rarely been documented or even investigated and, to justify conservation and management objectives based on the status indicators, it is essential to demonstrate their relationships to ecosystem functions and services. 2. The WFD requires member states to classify their surface waters aiming to achieve good ecological status of water bodies. How…

The cellular composition and morphological organization of the ventricular zone in the adult zebrafish telencephalon

2009

Über die Micromelerpetontidae (Amphibia: Temnospondyli). 1. Morphologie und Paläoökologie desMicromelerpeton credneri (Unter-Perm; SW-Deutschland)

1995

This paper presents a redescription of the most important features of the skull roof, palate, hyobranchial apparatus, and the postcranial skeleton ofMicromelerpeton credneri. It is possible to distinguish a temporal sequence of three morphotypes that can be differentiated by size and slight differences in the structure of the skull roof. They led a predaceous existence in the pelagial of three fairly deep lakes that had excellent potential for the preservation of fossils. Comparisons of the size-frequency distributions for specimens from different horizons indicate facultative paedomorphosis by means of progenesis. This phenomenon may be related to different trophic strategies and possible …

Über Carpus und Tarsus vonDeinotherium giganteum Kaup (Mamm., Proboscidea)

1962

Zusammengehorige Teile eines Skelettes vonDeinotherium giganteum Kp., welche im unteren Pliozan (Pontium) am Howenegg (Hegau, SW-Deutschland) ausgegraben worden sind, erlauben Beobachtungen zur Struktur des Hand- und Fusskelettes. Ein Verfahren zur graphischen Darstellung von Gelenkflachen wird geschildert, die Gultigkeit desCuvier’schen Korrelationsprinzips erortert.

Isolation and Characterization of Pathogen-Bearing Endosomes Enable Analysis of Endosomal Escape and Identification of New Cellular Cofactors of Infe…

2013

Many pathogens, including viruses, bacteria, as well as bacterial toxins, enter their target cells by endocytosis leading to accumulation of pathogenic and cellular proteins in endosomes. Here, we present detailed experimental instructions on isolation of endosomes after virus infection and their subsequent biomolecular characterization. The isolation of endosomes is based on discontinuous sucrose gradient centrifugation, where different endosomal compartments accumulate at a specific sucrose interface. This enables the enrichment and separation of the virus-interacting and co-internalized cell-surface receptors and membrane-associated proteins. The endosomal fractions can be further analyz…

Patterns and rates of nucleotide substitution, insertion and deletion in the endosymbiont of antsBlochmannia floridanus

2009

Genome reduction is a general process that has been studied in numerous symbiotic bacteria associated with insects. We investigated the last stages of genome degradation in Blochmannia floridanus, a mutualistic bacterial endosymbiont of the ant Camponotus floridanus. We determined the tempo (rates of insertion and deletion) and mode (size and number of insertion-deletion events) of the process in the last 200,000 years by analysing a total of 16 intergenic regions in several strains of this endosymbiont from different ant populations. We provide the first calculation of the reduction rate for noncoding DNA in this endosymbiont (2.2 x 10(-8) lost nucleotides/site/year) and compare it with th…

Different species and symbiotic genotypes of field rhizobia can nodulate Phaseolus vulgaris in Tunisian soils.

2002

Abstract A collection of 160 isolates of rhizobia nodulating Phaseolus vulgaris in three geographical regions in Tunisia was characterized by restriction fragment length polymorphism analysis of polymerase chain reaction (PCR)-amplified 16S rDNA, nifH and nodC genes. Nine groups of rhizobia were delineated: Rhizobium gallicum biovar (bv.) gallicum, Rhizobium leguminosarum bv. phaseoli and bv. viciae, Rhizobium etli bv. phaseoli, Rhizobium giardinii bv. giardinii, and four groups related to species of the genus Sinorhizobium, Sinorhizobium meliloti, Sinorhizobium medicae and Sinorhizobium fredii. The most abundant rhizobial species were R. gallicum, R. etli, and R. leguminosarum encompassing…