Search results for "CNO"
showing 10 items of 5223 documents
Recombinant laccase from Pediococcus acidilactici CECT 5930 with ability to degrade tyramine
2017
Biogenic amines degradation by bacterial laccases is little known, so we have cloned and heterologously expressed, in E. coli, a new laccase from Pediococcus acidilactici CECT 5930 (Lpa5930), a lactic acid bacterium commonly found in foods able to degrade tyramine. The recombinant enzyme has been characterized by physical and biochemical assays. Here we report the optimization of expression and purification procedures of this laccase. DNA encoding sequence of laccase from P. acidilactici was amplified by PCR and cloned into the expression plasmid pET28a for induction by isopropyl-β-D-thiogalactoipyranoside. Protein expression was performed in E. coli BL21(DE3) harboring pGro7 plasmid expres…
Spatial and Temporal Variability in Migration of a Soaring Raptor Across Three Continents
2019
Disentangling individual- and population-level variation in migratory movements is necessary for understanding migration at the species level. However, very few studies have analyzed these patterns across large portions of species' distributions. We compiled a large telemetry dataset on the globally endangered egyptian vulture neophron percnopterus (94 individuals, 188 completed migratory journeys), tracked across similar to 70% of the species' global range, to analyze spatial and temporal variability of migratory movements within and among individuals and populations. We found high migratory connectivity at large spatial scales (i.e., different subpopulations showed little overlap in winte…
Identification and molecular characterization of the high-affinity copper transporters family in Solanum lycopersicum
2021
Copper (Cu) plays a key role as cofactor in the plant proteins participating in essential cellular processes, such as electron transport and free radical scavenging. Despite high-affinity Cu transporters (COPTs) being key participants in Cu homeostasis maintenance, very little is known about COPTs in tomato (Solanum lycopersicum) even though it is the most consumed fruit worldwide and this crop is susceptible to suboptimal Cu conditions. In this study, a six-member family of COPT (SlCOPT1-6) was identified and characterized. SlCOPTs have a conserved architecture consisting of three transmembrane domains and β-strains. However, the presence of essential methionine residues, a methionine-enri…
Worldwide Scientific Research on Nanotechnology: A Bibliometric Analysis of Tendencies, Funding, and Challenges
2020
[EN] The main objective of this investigation was to analyze the scientific production in global research on nanotechnology, integrating scientific production, funding of studies, collaborations between countries, and the most cited publications. The source for obtaining the research papers for our analysis was the Science Citation Index Expanded from the Web of Science. A total of 3546 documents were extracted during the period of 1997-2018. Food science & technology, chemistry (applied and analytical), spectroscopy, and agriculture appeared as the main areas where the articles were published. Most prolific and cited journals were Analytical Methods, Journal of Agricultural and Food Chemis…
Integrative effects of vine water relations and grape ripeness level of vitis vinifera L. cv. Shiraz/Richter 99. II. grape composition and wine quali…
2016
Regulation of grapevine water status is a common practice to manipulate grape composition and wine quality. In this investigation the effect of plant water status (two field water capacity-based irrigation levels, 75% and 100%, applied at single and combined vine developmental stages) and ripeness level (harvesting at different soluble solid levels) on grape composition and wine quality of Vitis vinifera L. cv. Shiraz/ Richter 99 was determined. Integrative effects of vine water relations and grape ripeness level, specifically in a Mediterranean high winter rainfall area, have not yet been investigated systematically. Source:sink mechanisms and dynamics and compositional and physical change…
Defoliation of two-wire vertical trellis: effect on grape quality
2017
ABSTRACTBasal leaves were removed from Cabernet Sauvignon vines trained to a two-wire vertical trellis at fruit set and at veraison. Leaf removal did not modify total soluble solids and titratable acidity at harvest. Defoliation at fruit set of lower cordon recovered the grape anthocyanin composition gap between upper and lower cordons and that produced a positive effect on anthocyanin synthesis. Hence, control of the upper cordon and defoliation of fruit set of the lower cordon treatments showed comparable values of anthocyanins. Defoliation at veraison did not produce any appreciable effect. This study shows that skin anthocyanin composition in a two-wire vertical trellis can be modified …
Anaerobic membrane bioreactors (AnMBR) treating urban wastewater in mild climates
2020
[EN] Feasibility of an AnMBR demonstration plant treating urban wastewater (UWW) at temperatures around 25-30 degrees C was assessed during a 350-day experimental period. The plant was fed with the effluent from the pretreatment of a full-scale municipal WWTP, characterized by high COD and sulfate concentrations. Biodegradability of the UWW reached values up to 87%, although a portion of the biodegradable COD was consumed by sulfate reducing organisms. Effluent COD remained below effluent discharge limits, achieving COD removals above 90%. System operation resulted in a reduction of sludge production of 36-58% compared to theoretical aerobic sludge productions. The membranes were operated a…
Behavior of mixed Chlorophyceae cultures under prolonged dark exposure. Respiration rate modeling
2016
[EN] The behavior of three different microalgal cultures, when exposed for a long period (>48 h) to dark conditions, was studied with a methodology based on respirometry. The cultures were transferred to darkness and the oxygen evolution in the reactors was monitored after successive air injections. Several sequential oxygen uptake rates were thus calculated and a respiration constant, assuming a first order decay of a fraction of the biomass, was obtained by calibration. Initial specific oxygen uptake rates were in the range of 0.9 5.1 mg O2 g TSS−1 h−1 and dark respiration constants in the range of 0.005 0.018 h−1.
MAB2.0 project: Integrating algae production into wastewater treatment
2018
Abstract Different species of microalgae are highly efficient in removing nutrients from wastewater streams and are able to grow using flue gas as a CO2 source. These features indicate that application of microalgae has a promising outlook in wastewater treatment. However, practical aspects and process of integration of algae cultivation into an existing wastewater treatment line have not been investigated. The Climate-KIC co-funded Microalgae Biorefinery 2.0 project developed and demonstrated this integration process through a case study. The purpose of this paper is to introduce this process by phases and protocols, as well as report on the challenges and bottlenecks identified in the cas…
Warmer temperatures reduce the influence of an important keystone predator
2017
Predator–prey interactions may be strongly influenced by temperature variations in marine ecosystems. Consequently, climate change may alter the importance of predators with repercussions for ecosystem functioning and structure. In North-eastern Pacific kelp forests, the starfish Pycnopodia helianthoides is known to be an important predator of the purple sea urchin Strongylocentrotus purpuratus. Here we investigated the influence of water temperature on this predator–prey interaction by: (i) assessing the spatial distribution and temporal dynamics of both species across a temperature gradient in the northern Channel Islands, California, and (ii) investigating how the feeding rate of P. heli…