Search results for "CQ"

Application of a 5-bromo-2′-deoxyuridine ELISA for measuring the lymphoproliferative response to human cytomegalovirus in HIV-1-infected patients

2002

Assessment of the lymphoproliferative response to human cytomegalovirus (HCMV) may help to identify human immunodeficiency virus (HIV)-1-infected patients at high risk of developing HCMV end-organ disease. The tritiated thymidine ([3H]-TdR)-incorporation assay is the gold standard for measuring lymphoproliferative responses, though it is unsuitable as a routine laboratory procedure. An alternative non-radioactive technique, a 5-bromo-2'-deoxyuridine (BrdU) enzyme-linked immunosorbent assay, was applied for measuring T-cell proliferation in response to HCMV. Stimulation of either 1 x 10(5) or 5 x 10(4) peripheral blood mononuclear cells (PBMCs)/well with 10 PFU/well (before inactivation) of …

Presence of Epstein-Barr virus, cytomegalovirus and human papillomavirus in normal oral mucosa of HIV-infected and renal transplant patients

2001

OBJECTIVES: To determine the prevalence of EBV-DNA,CMV-DNA and HPV-DNA in oral healthy mucosa of HIV-infected and renal transplant patients. To associate the detection of viral genomes with laboratory parameters of immunodeficiency, gender, antiretroviral and immunosuppressive therapy. DESIGN: A cross-sectional analysis of lingual and buccal cytobrushings from HIV-infected and renal transplant patients. SUBJECTS AND METHODS: Lingual and buccal cytobrushings were obtained from clinically normal oral mucosa of 57 HIV+, 40 renal transplant patients and 30 healthy uninfected controls, all matched for age at baseline of examination. Presence of EBV-, CMV- and HPV-DNA was assessed by polymerase c…

Water Level Measurement Locations for Coastal Aquifer Management

2003

The coefficient of variation of the optimal cost (CVOC) method is applied to the Acqua dei Corsari coastal aquifer in Sicily's north coast to find the best point for a new water-level measurement location. The purpose of collecting data from the new measurement location is to reduce uncertainty associated with a stochastic management model for salt-water intrusion control. Assuming that uncertainty mainly stems from spatial variability of permeability, the structural parameters of the stochastic spatial permeability field are estimated using data from a nearby aquifer with similar geological properties. It is first shown through sensitivity analysis that the lack of previous information inh…

Proteinaceous and oligosaccharidic elicitors induce different calcium signatures in the nucleus of tobacco cells.

2005

We previously reported elevated cytosolic calcium levels in tobacco cells in response to elicitors [D. Lecourieux, C. Mazars, N. Pauly, R. Ranjeva, A. Pugin, Analysis and effects of cytosolic free calcium elevations in response to elicitors in Nicotiana plumbaginifolia cells, Plant Cell 14 (2002) 2627-2641]. These data suggested that in response to elicitors, Ca2+, as a second messenger, was involved in both systemic acquired resistance (RSA) and/or hypersensitive response (HR) depending on calcium signature. Here, we used transformed tobacco cells with apoaequorin expressed in the nucleus to monitor changes in free nuclear calcium concentrations ([Ca2+](nuc)) in response to elicitors. Two …

Purification, crystallization and preliminary X-ray studies of sylvaticin, an elicitin-like protein from Pythium sylvaticum.

2003

Sylvaticin belongs to the elicitin family. These 10 kDa oomycetous proteins induce a hypersensitive response in plants, including necrosis and cell death, but subsequently leading to a non-specific systemic acquired resistance (SAR) against other pathogens. Sylvaticin has been crystallized using PEG 2000 MME as a precipitant agent in the presence of nickel chloride. The crystals belong to space group C2, with unit-cell parameters a = 99.29, b = 25.67, c = 67.45 A, beta = 99.66 degrees. Diffraction data were recorded to 2.1 A resolution at a synchrotron-radiation source.

The Elicitor Cryptogein Blocks Glucose Transport in Tobacco Cells

2002

Abstract Cryptogein is a 10-kD protein secreted by the oomycetePhytophthora cryptogea that induces a hypersensitive response on tobacco (Nicotiana tabacum var. Xanthi) plants and a systemic acquired resistance against various pathogens. The mode of action of this elicitor has been studied using tobacco cell suspensions. Our previous data indicated that within minutes, cryptogein signaling involves various events including changes in ion fluxes, protein phosphorylation, sugar metabolism, and, eventually, cell death. These results suggested that transport of sugars could be affected and, thus, involved in the complex relationships between plant and microorganisms via elicitors. This led us to…

Update I. A systematic review on the efficacy and safety of chloroquine/hydroxychloroquine for COVID-19

2020

Purpose To assess efficacy and safety of chloroquine (CQ)/hydroxychloroquine (HCQ) for treatment or prophylaxis of COVID-19 in adult humans. Materials and methods MEDLINE, PubMed, EMBASE and two pre-print repositories (bioRxiv, medRxiv) were searched from inception to 8th June 2020 for RCTs and nonrandomized studies (retrospective and prospective, including single-arm, studies) addressing the use of CQ/HCQ in any dose or combination for COVID-19. Results Thirty-two studies were included (6 RCTs, 26 nonrandomized, 29,192 participants). Two RCTs had high risk, two ‘some concerns’ and two low risk of bias (Rob2). Among nonrandomized studies with comparators, nine had high risk and five moderat…

“Operative propoals for resisting the phenomenon of the seawater intrusion along the coast of the eastern Sicily"

2003

Experimental Textual-learning Activities for an L2 Italian Initial-literacy Classroom

2019

The paper describes some specific textual learning activities developed for an L2 Italian initial literacy classroom and, in particular, for illiterate multilinguals (mostly unaccompanied foreign minors). The activities belong to an experimental proposal. It consists of a specific textual teaching module integrated with a second-language initial literacy course. The aim was to get the learner immediately accustomed to some types of text, that have been chosen to be suitable for his profile and his needs, as well as perfectly integrated with the contexts and vocabulary proposed in the classroom. Because of the minimum level, the texts were mostly discontinuous. Based on the importance of lea…

THE DIAGNOSIS OF ROAD SURFACE DISTRESSES THROUGH IMAGE-BASED MODELING TECHNIQUES. EXPERIMENTAL SURVEY ON LABORATORY-RUTTED SAMPLES

2015

In the past few years, there has been a drastic increase in the use of image-based modeling (IBM) techniques to create high quality, reality-based 3D models. The low costs of these techniques, as well as their attractive visual quality, have led many researchers and professionals to invest their energy and resources in several tests. IBM is rarely used in the field of road surface distresses as diagnosis is usually performed using other techniques and devices. Road safety statistics reveal that about a half of the total number of accidents occur mainly due to the deterioration of the pavement. The goal of effective road network management is often incompatible with economic resources design…