6533b7d0fe1ef96bd125b898
RESEARCH PRODUCT
Purification, crystallization and preliminary X-ray studies of sylvaticin, an elicitin-like protein from Pythium sylvaticum.
Thierry PrangéJean-pierre BleinL. CardinMarie-bernard LascombeMarie-louise MilatMichel Ponchetsubject
Hypersensitive responseStereochemistryProtein ConformationPythiumBiologyCrystallography X-Raylaw.inventionPolyethylene GlycolsProtein structureStructural BiologylawNickelPEG ratioCrystallizationFuransAlgal ProteinsX-rayProteinsElicitinGeneral Medicinebiology.organism_classificationCrystallographySolventsPythium sylvaticumSystemic acquired resistanceSynchrotronsdescription
Sylvaticin belongs to the elicitin family. These 10 kDa oomycetous proteins induce a hypersensitive response in plants, including necrosis and cell death, but subsequently leading to a non-specific systemic acquired resistance (SAR) against other pathogens. Sylvaticin has been crystallized using PEG 2000 MME as a precipitant agent in the presence of nickel chloride. The crystals belong to space group C2, with unit-cell parameters a = 99.29, b = 25.67, c = 67.45 A, beta = 99.66 degrees. Diffraction data were recorded to 2.1 A resolution at a synchrotron-radiation source.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2003-09-24 | Acta crystallographica. Section D, Biological crystallography |