0000000000134030

AUTHOR

Marie-bernard Lascombe

showing 2 related works from this author

Purification, crystallization and preliminary X-ray studies of sylvaticin, an elicitin-like protein from Pythium sylvaticum.

2003

Sylvaticin belongs to the elicitin family. These 10 kDa oomycetous proteins induce a hypersensitive response in plants, including necrosis and cell death, but subsequently leading to a non-specific systemic acquired resistance (SAR) against other pathogens. Sylvaticin has been crystallized using PEG 2000 MME as a precipitant agent in the presence of nickel chloride. The crystals belong to space group C2, with unit-cell parameters a = 99.29, b = 25.67, c = 67.45 A, beta = 99.66 degrees. Diffraction data were recorded to 2.1 A resolution at a synchrotron-radiation source.

Hypersensitive responseStereochemistryProtein ConformationPythiumBiologyCrystallography X-Raylaw.inventionPolyethylene GlycolsProtein structureStructural BiologylawNickelPEG ratioCrystallizationFuransAlgal ProteinsX-rayProteinsElicitinGeneral Medicinebiology.organism_classificationCrystallographySolventsPythium sylvaticumSystemic acquired resistanceSynchrotronsActa crystallographica. Section D, Biological crystallography
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Crystallization and preliminary X-ray studies of oligandrin, a sterol-carrier elicitor fromPythium oligandrum

2000

Oligandrin is a 10 kDa acidic protein produced by the fungus micromycete Pythium oligandrum and is a member of the alpha-elicitin group, with sterol- and lipid-carrier properties. Oligandrin has been crystallized at 290 K using PEG 4000 as a precipitant. A cholesterol complex was obtained under the same conditions. The space group of the crystals at low temperature (100 K) is C222, with unit-cell parameters a = 94.0, b = 171.1, c = 55.3 A. Four molecules are present in the asymmetric unit. Data from the free and cholesterol-complexed forms were recorded at synchrotron sources to resolutions of 2.4 (uncomplexed) and 1.9 A (complexed), respectively.

Protein ConformationPythiumElicitinGeneral MedicineBiologyCrystallography X-Raybiology.organism_classificationSterolElicitorlaw.inventionFungal ProteinsSterolsCrystallographyCholesterolSterol carrier proteinStructural BiologylawPEG ratioIntercellular Signaling Peptides and ProteinsMoleculeElectrophoresis Polyacrylamide GelCrystallizationCarrier ProteinsCrystallizationPythium oligandrumActa Crystallographica Section D Biological Crystallography
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