Search results for "CRT"

Alix regulates egress of hepatitis B virus naked capsid particles in an ESCRT-independent manner

2010

Hepatitis B virus (HBV) is an enveloped DNA virus that exploits the endosomal sorting complexes required for transport (ESCRT) pathway for budding. In addition to infectious particles, HBV-replicating cells release non-enveloped (nucleo)capsids, but their functional implication and pathways of release are unclear. Here, we focused on the molecular mechanisms and found that the sole expression of the HBV core protein is sufficient for capsid release. Unexpectedly, released capsids are devoid of a detectable membrane bilayer, implicating a non-vesicular exocytosis process. Unlike virions, naked capsid budding does not require the ESCRT machinery. Rather, we identified Alix, a multifunctional …

Analysis of Energy Efficiency of Different Setups Able to Perform Single Point Incremental Forming (SPIF) Processes

2014

Abstract Production processes, as used for discrete part manufacturing, are responsible for a substantial part of the environmental impact of products, but are still poorly documented in terms of environmental impact. The present paper proposes an energy analysis of single point incremental forming processes (SPIF). The electrical energy consumption of two different setups have been considered and compared. In particular the power demand of one of the most used machine tools architecture able to perform SPIF process, namely a CNC milling machine, has been measured. The obtained results have been compared with the ones obtained for an innovative high speed incremental forming processes perfo…

Finansinis stabilumas ir koncentracija: besivystančios Europos tyrimas

2016

Šiame straipsnyje tiriamas bankų sektoriaus koncentracijos poveikis finansiniam stabilumui naudojant 134 komercinių bankų iš 17 Vidurio ir Rytų Europos šalių 2007-2012 m. laikotarpio duomenis. Empirinio tyrimo rezultatai pabrėžia vadinamąją „koncentracijos trapumo“ hipotezę, parodydami, kad aukštas koncentracijos lygis bankų sektoriuje yra siejamas su didesne bankų, ypač mažesniųjų, įsipareigojimų neįvykdymo tikimybe. Taip pat parodoma, kad neigiamas koncentracijos poveikis gali būti sumažintas taikant griežtą priežiūros sistemą. Pirmiausia - griežtas oficialus bankų sektoriaus priežiūros įgaliojimas. Antra - skirtingi priimančiosios šalies ir buveinės šalies priežiūros įgaliojimai gali žym…

Meshless Electrophysiological Modeling of Cardiac Resynchronization Therapy—Benchmark Analysis with Finite-Element Methods in Experimental Data

2022

Computational models of cardiac electrophysiology are promising tools for reducing the rates of non-response patients suitable for cardiac resynchronization therapy (CRT) by optimizing electrode placement. The majority of computational models in the literature are mesh-based, primarily using the finite element method (FEM). The generation of patient-specific cardiac meshes has traditionally been a tedious task requiring manual intervention and hindering the modeling of a large number of cases. Meshless models can be a valid alternative due to their mesh quality independence. The organization of challenges such as the CRT-EPiggy19, providing unique experimental data as open access, enables b…

2014

The hepatitis B virus (HBV) is an enveloped DNA virus that replicates via reverse transcription of its pregenomic RNA (pgRNA). Budding of HBV is supposed to occur at intracellular membranes and requires scission functions of the endosomal sorting complex required for transport (ESCRT) provided by ESCRT-III and VPS4. Here, we have investigated the impact of the upstream-acting ESCRT-I and ESCRT-II complexes in HBV morphogenesis. RNA interference knockdown of the ESCRT-I subunits TSG101 and VPS28 did not block, but rather stimulate virus release. In contrast, RNAi-mediated depletion of the ESCRT-II components EAP20, EAP30 and EAP45 greatly reduced virus egress. By analyzing different steps of…

Hepatitis B virus maturation is sensitive to functional inhibition of ESCRT-III, Vps4, and gamma 2-adaptin.

2007

ABSTRACT Hepatitis B virus (HBV) is an enveloped DNA virus that presumably buds at intracellular membranes of infected cells. HBV budding involves two endocytic host proteins, the ubiquitin-interacting adaptor γ2-adaptin and the Nedd4 ubiquitin ligase. Here, we demonstrate that HBV release also requires the cellular machinery that generates internal vesicles of multivesicular bodies (MVBs). In order to perturb the MVB machinery in HBV-replicating liver cells, we used ectopic expression of dominant-negative mutants of different MVB components, like the ESCRT-III complex-forming CHMP proteins and the Vps4 ATPases. Upon coexpression of mutated CHMP3, CHMP4B, or CHMP4C forms, as well as of ATPa…

The PLVC display color characterization model revisited

2008

This work proposes a study of the Piecewise Linear assuming Variation in Chromaticity (PLVC) dis- play color characterization model. This model has not been widely used as the improved accuracy compared with the more common PLCC (Piecewise Linear assuming Chromaticity Constancy) model is not significant for CRT (Cathode Ray Tube) display technology, and it requires more computing power than this model. With today's computers, computational complexity is less of a problem, and today's display technologies show a different colori- metric behavior than CRTs. The main contribution of this work is to generalize the PLVC model to multiprimary displays and to provide extensive experimental results…

The importance of being “responder” in cardiac resynchronization therapy

2016

Abdominal infections in the intensive care unit: characteristics, treatment and determinants of outcome

2014

Background: Abdominal infections are frequent causes of sepsis and septic shock in the intensive care unit (ICU) and are associated with adverse outcomes. We analyzed the characteristics, treatments and outcome of ICU patients with abdominal infections using data extracted from a one-day point prevalence study, the Extended Prevalence of Infection in the ICU (EPIC) II.Methods: EPIC II included 13,796 adult patients from 1,265 ICUs in 75 countries. Infection was defined using the International Sepsis Forum criteria. Microbiological analyses were performed locally. Participating ICUs provided patient follow-up until hospital discharge or for 60 days.Results: Of the 7,087 infected patients, 1,…

PspA adopts an ESCRT-III-like fold and remodels bacterial membranes

2020

SummaryPspA is the main effector of the phage shock protein (Psp) system and preserves the bacterial inner membrane integrity and function. Here, we present the 3.6 Å resolution cryo-EM structure of PspA assembled in helical rods. PspA monomers adopt a canonical ESCRT-III fold in an extended open conformation. PspA rods are capable of enclosing lipids and generate positive membrane curvature. Using cryo-EM we visualized how PspA remodels membrane vesicles into μm-sized structures and how it mediates the formation of internalized vesicular structures. Hot spots of these activities are zones derived from PspA assemblies, serving as lipid transfer platforms and linking previously separated lip…