Search results for "Caco-2"
showing 10 items of 203 documents
Efecto del arsénico inorgánico presente en los alimentos sobre el epitelio intestinal
2018
El arsénico es un metaloide tóxico ampliamente distribuido en el medioambiente. Su forma inorgánica [As(III) y As(V)] es la más tóxica encontrada en agua y alimentos. La exposición a arsénico inorgánico, descrita en numerosas zonas del planeta y que en la actualidad se estima que afecta a 200 millones de personas, aumenta la incidencia de determinados tipos de cáncer y otras patologías no cancerígenas. Teniendo en cuenta que la principal vía de exposición es la oral, el tracto gastrointestinal se considera la puerta de entrada de este tóxico al organismo; sin embargo, los estudios para determinar los efectos tóxicos del arsénico inorgánico a nivel intestinal son escasos. Esta tesis doctoral…
Estudios in vitro de mecanismos de transporte y toxicidad de especies arsenicales a nivel intestinal
2013
El arsénico es un metaloide ampliamente distribuido en el medioambiente, del cual existen numerosas formas químicas con características toxicológicas muy diferentes. En la actualidad, las formas inorgánicas son la que mayor interés despiertan, ya que se consideran carcinogénicas para el hombre. Se estima que alrededor de 140 millones de personas están afectadas por consumo de agua contaminada con niveles de arsénico que superan los máximos recomendados por la Organización Mundial de la Salud (10 µg/L). Los problemas de salud derivados de la exposición a este contaminante alcanzan gran magnitud en ciertas áreas geográficas de India, Bangladesh y Argentina, donde por características geológica…
Inhibition of Folic Acid Uptake by Catechins and Tea Extracts in Caco-2 Cells
2006
In this present study it was aimed to determine whether the catechins contained in green tea and the whole extracts of Camellia sinensis (Theaceae) inhibit the uptake of folic acid by Caco-2 cell monolayers. Our results indicate that (-)-epigallocatechin 3-gallate (EGCG) and (-)-epicatechin 3-gallate (ECG) inhibit cellular folic acid uptake with IC50 values of 34.8 micromol/L and 30.8 micromol/L, respectively. Furthermore, green and black tea extracts were also found to inhibit folic acid uptake with IC50 values of approximately 7.5 and 3.6 mg/mL, respectively. According to these results, simultaneous intake of tea and folic acid may inhibit intestinal folic acid absorption. The consequence…
Mercury and selenium in fish and shellfish: Occurrence, bioaccessibility and uptake by Caco-2 cells
2012
This study evaluates Hg and Se concentrations and bioaccessibility (element solubilised after simulated gastrointestinal digestion) in 16 raw seafood species consumed in Spain. The concentrations varied greatly (Hg, 3.8-1621 ng/g wet weight, ww; Se, 84-1817 ng/g ww). Only one sample of swordfish exceeded the Hg limit permitted in Spain (1mg/kg), and for this sample the Hg/Se molar ratio and Se Health Benefit Value food safety criteria also indicated the presence of a risk. Bioaccessibility of Hg (35-106%) and Se (17-125%) was very variable and the Hg/Se molar ratio in the bioaccessible fraction was less than one for all samples. Transport by Caco-2 cells, an intestinal epithelium model, was…
Etude du rôle des jonctions serrées dans l’interaction de Candida albicans avec les cellules entérocytaires Caco-2
2012
Rapport de stage de Master 2 EA MERS Hors CT hors EJ; Master
Photocrosslinking of dextran and polyaspartamide derivatives: a combination suitable for colon-specific drug delivery.
2007
Abstract The aim of this study was to prepare and characterize novel hydrogels with polysaccharide–polyaminoacid structure, able to undergo an enzymatic hydrolysis in the colon and potentially useful for treating inflammatory bowel diseases (IBD). Starting materials were methacrylated dextran (DEX-MA) and methacrylated α,β-poly(N-2-hydroxyethyl)- dl -aspartamide (PHM). These polymers were photocrosslinked by exposure of their aqueous solutions at 313 nm without photoinitiators. Different samples, shaped as microparticles, were obtained as a function of polymer concentration and irradiation time. FT-IR analysis confirmed the occurrence of a co-crosslinking between DEX-MA and PHM in all exper…
New 1,2,4-oxadiazole nortopsentin derivatives with cytotoxic activity
2019
New analogs of nortopsentin, a natural 2,4-bis(3&prime
Transepithelial transport of betalainic phytochemicals in Caco-2 cell monolayers.
2010
Effect of caseinophosphopeptides added to fruit beverages upon ferritin synthesis in Caco-2 cells
2010
Ferritin synthesis was evaluated in iron sulphate solutions and in fruit beverages supplemented with iron, with/without zinc in the presence of caseinophosphopeptides (CPPs), based on a system combining simulated gastrointestinal digestion and the Caco-2 cell model. In iron sulphate solutions with/without zinc, the addition of CPPs had no effect upon ferritin synthesis. When CPPs were added to the bioaccessible fraction of fruit beverages supplemented with iron, ferritin synthesis increased in the same proportion as with the addition of milk. The addition of zinc to fruit beverages supplemented with iron and to iron sulphate solutions decreased ferritin synthesis. Nevertheless, in fruit bev…
Ferritin synthesis by Caco-2 cells as an indicator of iron bioavailability: Application to milk-based infant formulas
2007
The bioavailability of iron from milk-based infant formulas was estimated by an in vitro system including enzymatic digestion, iron uptake by Caco-2 cells and ferritin determination via an enzymoimmunoassay (ELISA). Positive correlations (p < 0.01) were found between the Fe(II) added to Caco-2 cells and ferritin synthesis and between the amount of dialyzed iron added to the cell culture and ferritin synthesis. The comparison of the bioavailability of iron from different milk-based formulas showed that adapted formulas having the same composition but differing in the iron salts added yielded similar ferritin levels. The same happened with follow-up formulas differing only in the presence or …