Search results for "Calcium"

showing 10 items of 1740 documents

Seagrass ecosystem response to long-term high CO2 in a Mediterranean volcanic vent

2014

We examined the long-term effect of naturally acidified water on a Cymodocea nodosa meadow growing at a shallow volcanic CO2 vent in Vulcano Island (Italy). Seagrass and adjacent unvegetated habitats growing at a low pH station (pH = 7.65 ± 0.02) were compared with corresponding habitats at a control station (pH = 8.01 ± 0.01). Density and biomass showed a clear decreasing trend at the low pH station and the below- to above-ground biomass ratio was more than 10 times lower compared to the control. C content and delta 13C of leaves and epiphytes were significantly lower at the low pH station. Photosynthetic activity of C. nodosa was stimulated by low pH as seen by the significant increase in…

Ocean Acidification International Coordination Centre (OA-ICC)TemperateSalinityChlorophyll ainorganicAlkalinityLight saturation point standard errorPhotosynthetic quantum efficiencyMediterranean Sea Acidification in a Changing Climate MedSeATemperature waterCarbon inorganic dissolvedCalculated using seacarb after Nisumaa et al 2010IrradianceRespiration rate carbonAragonite saturation stateBiomassAlkalinity totalIrradiance standard errortotalCO2 ventCymodocea nodosapHRespirationEpiphytes loadMaximum photochemical quantum yield of photosystem II standard errorNet community production of carbonTemperaturePartial pressure of carbon dioxide (water) at sea surface temperature (wet air) standard errordissolvedRespiration rate carbon standard errorCarbonate ionMaximum photochemical quantum yield of photosystem IIPartial pressure of carbon dioxide (water) at sea surface temperature (wet air)Net community production of carbon standard errorIlluminance standard errorSoft bottom communitystandard errorCarbon inorganic dissolved standard errorRespiration rateElectron transport rate standard errorEarth System Researchδ13CPhotosynthetic quantum efficiency standard errorField observationChlorophyll a standard errorGross primary production of carbonBiomass standard errorCalcium carbonatePotentiometric titrationCalcite saturation stateShoot densityPotentiometricwaterIlluminanceOxygen standard errorBenthosAlkalinity total standard errorMediterranean Sea Acidification in a Changing Climate (MedSeA)Electron transport rateLight saturation pointOcean Acidification International Coordination Centre OA ICCMediterranean SeaGross primary production of carbon standard errorBicarbonate ionSoft-bottom communityδ13C standard errorTemperature water standard errorCalculated using seacarb after Nisumaa et al. (2010)Primary production PhotosynthesisSpeciespH standard errorCarbonate system computation flagloadPrimary production/PhotosynthesisFugacity of carbon dioxide (water) at sea surface temperature (wet air)CarbonBiomass/Abundance/Elemental compositionTreatmentEpiphytes load standard errorOxygenPartial pressure of carbon dioxide water at sea surface temperature wet airEpiphytes loadCarbon dioxideCarbon standard errorEntire communityFugacity of carbon dioxide water at sea surface temperature wet airGroupBiomass Abundance Elemental compositionCoast and continental shelfEpiphytesShoot density standard errorCalcium carbonate standard error
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Seawater carbonate chemistry and coralline algal diversity

2021

Calcified coralline algae are ecologically important in rocky habitats in the marine photic zone worldwide and there is growing concern that ocean acidification will severely impact them. Laboratory studies of these algae in simulated ocean acidification conditions have revealed wide variability in growth, photosynthesis and calcification responses, making it difficult to assess their future biodiversity, abundance and contribution to ecosystem function. Here, we apply molecular systematic tools to assess the impact of natural gradients in seawater carbonate chemistry on the biodiversity of coralline algae in the Mediterranean and the NW Pacific, link this to their evolutionary history and …

Ocean Acidification International Coordination Centre (OA-ICC)TemperateSalinityCommunity composition and diversityBicarbonate ion standard deviationinorganicAlkalinity total standard deviationAlkalinityExperimentTemperature waterCarbon inorganic dissolvedCalculated using seacarb after Nisumaa et al 2010Aragonite saturation stateNorth PacificMarine habitatAlkalinity totaltotalCO2 ventpHTemperaturedissolvedCarbonate ionPartial pressure of carbon dioxide (water) at sea surface temperature (wet air)Calcium carbonate standard deviationstandard errorEarth System Researchstandard deviationField observationCalcium carbonateCoverageCalcite saturation stateLocationwaterPartial pressure of carbon dioxideSiteRocky-shore communityCalcium carbonate massAragonite saturation state standard deviationBenthosSalinity standard deviationOcean Acidification International Coordination Centre OA ICCMediterranean SeaCarbon inorganic dissolved standard deviationCalcite saturation state standard deviationTypeBicarbonate ionCalculated using seacarb after Nisumaa et al. (2010)Coverage standard errorfungiEvent labelPartial pressure of carbon dioxide standard deviationCarbonate system computation flagpH standard deviationCarbonate ion standard deviationFugacity of carbon dioxide (water) at sea surface temperature (wet air)CarbonPartial pressure of carbon dioxide water at sea surface temperature wet airCarbon dioxideRocky shore communityEntire communityFugacity of carbon dioxide water at sea surface temperature wet airCoast and continental shelfSpecies richness
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Ochratoxin A in the morning and afternoon portions of urine from Coimbra and Valencian populations.

2008

Abstract The widespread contamination of foodstuffs and beverages by mycotoxins, such as ochratoxin A (OTA), has made the monitoring of human contamination levels essential. By using a sensitive, accurate and speedy method that combines extraction with 5% NaHCO 3 , immunoaffinity column clean-up and HPLC with fluorescence detection, the human exposure to OTA through urine analysis can be monitored. This method is less invasive than blood monitoring and has the potential to be a good marker of human exposure. The limit of quantification of the method was 0.007 ng/mL of urine, with recoveries of OTA, from urine samples spiked at levels between 0.02 and 0.1 ng/mL, higher than 91% with RSD lowe…

Ochratoxin ACoimbraAdultMaleTime FactorsAdolescentPopulationFood ContaminationUrineBiologyToxicologychemistry.chemical_compoundAnimal scienceHumansHuman urineeducationMycotoxinOchratoxinChromatography High Pressure LiquidMorningAgedDetection limiteducation.field_of_studyPortugalReproducibility of ResultsOchratoxin AContaminationMiddle AgedCalcium Channel BlockersOchratoxinschemistrySpainEnvironmental chemistryFemaleValenciaEnvironmental MonitoringToxicon : official journal of the International Society on Toxinology
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Interactions of odorants with olfactory receptors and receptor neurons match the perceptual dynamics observed for woody and fruity odorant mixtures

2012

The present study aimed to create a direct bridge between observations on peripheral and central responses to odorant mixtures and their components. Three experiments were performed using mixtures of fruity (isoamyl acetate; ISO) and woody (whiskey lactone; WL) odorants known to contribute to some of the major notes in Burgundy red wine. These experiments consisted of (i) calcium imaging of human embryonic kidney cells (HEK293T) transfected with olfactory receptors (ORs); (ii) single-unit electrophysiological recordings from olfactory receptor neurons (ORNs) and analyses of electro-olfactogram (EOG) responses in the rat nose in vivo; and (iii) psychophysical measurements of the perceived in…

Olfactory system0303 health sciencesOlfactory receptorGeneral NeuroscienceIsoamyl acetateStimulation03 medical and health scienceschemistry.chemical_compoundElectrophysiology0302 clinical medicineCalcium imagingmedicine.anatomical_structurechemistryBiochemistryIn vivomedicinesense organsReceptor030217 neurology & neurosurgery030304 developmental biologyEuropean Journal of Neuroscience
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Characterization of somatostatin- and cholecystokinin-immunoreactive periglomerular cells in the rat olfactory bulb.

2005

Periglomerular cells (PG) are interneurons of the olfactory bulb (OB) that modulate the first synaptic relay of the olfactory information from the olfactory nerve to the dendrites of the bulbar principal cells. Previous investigations have pointed to the heterogeneity of these interneurons and have demonstrated the presence of two different types of PG. In the rat OB, type 1 PG receive synaptic contacts from the olfactory axons and are γ-aminobutyric acid (GABA)-ergic, whereas type 2 PG do not receive synaptic contacts from the olfactory axons and are GABA immunonegative. In this study, we analyze and characterize neurochemically a group of PG that has not been previously classified either …

Olfactory systemCalbindinsNeuropilOlfactory NervePresynaptic TerminalsSynaptic MembranesNeuropeptideOlfactionBiologyCalbindinSynaptic TransmissionS100 Calcium Binding Protein GOlfactory nerveMicroscopy Electron TransmissionInterneuronsNeural PathwaysNeuropilmedicineAnimalsRats Wistargamma-Aminobutyric AcidGeneral NeuroscienceNeural InhibitionImmunohistochemistryOlfactory BulbOlfactory bulbRatsSmellmedicine.anatomical_structurenervous systemFemaleCalretininCholecystokininSomatostatinNeuroscienceThe Journal of comparative neurology
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Coexpression of neurocalcin with other calcium-binding proteins in the rat main olfactory bulb

1999

The distribution patterns of four calcium-binding proteins (CaBPs)-calbindin D-28k (CB), calretinin (CR), neurocalcin (NC), and parvalbumin (PV)-in the rat main olfactory bulb were compared, and the degrees ofcolocalization of NC with the other CaBPs were determined by using double immunocytochemical techniques. All investigated CaBPs were detected in groups of periglomerular cells and Van Gehuchten cells, whereas other cell types expressed some of the investigated proteins but not all four. Double-labeling techniques demonstrated the colocalization of NC with CB, CR, or PV in periglomerular cells, whereas each neurochemical group constituted entirely segregated populations in the remaining…

Olfactory systemeducation.field_of_studyNeurocalcinbiologyGeneral NeurosciencePopulationColocalizationOlfactory bulbCell biologynervous systemCalcium-binding proteinbiology.proteinCalretinineducationNeuroscienceParvalbuminThe Journal of Comparative Neurology
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MP-02.07 Calcium Depending Bone Metastases in Renal Cell Carcinoma

2011

Oncologymedicine.medical_specialtychemistryRenal cell carcinomabusiness.industryUrologyInternal medicinemedicineCancer researchchemistry.chemical_elementCalciummedicine.diseasebusinessUrology
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Navigating the translational roadblock: Towards highly specific and effective all-optical interrogations of neural circuits

2020

AbstractTwo-photon (2-P) all-optical approaches combine in vivo 2-P calcium imaging and 2-P optogenetic modulations and have the potential to build a framework for network-based therapies, e.g. for rebalancing maladaptive activity patterns in preclinical models of neurological disorders. Here, our goal was to tailor these approaches for this purpose: Firstly, we combined in vivo juxtacellular recordings and GCaMP6f-based 2-P calcium imaging in layer II/III of mouse visual cortex to tune our detection algorithm towards a 100 % specific identification of AP-related calcium transients. False-positive-free detection was achieved at a sensitivity of approximately 73 %. To further increase specif…

Opsingenetic structuresComputer scienceCortical neuronsOptogeneticsPhotostimulationCalcium imagingVisual cortexmedicine.anatomical_structureIn vivoGCaMPmedicineExcitatory postsynaptic potentialBiological neural networkNeuroscience
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Calcium signaling in plant cell organelles delimited by a double membrane.

2006

AbstractIncreases in the concentration of free calcium in the cytosol are one of the general events that relay an external stimulus to the internal cellular machinery and allow eukaryotic organisms, including plants, to mount a specific biological response. Different lines of evidence have shown that other intracellular organelles contribute to the regulation of free calcium homeostasis in the cytosol. The vacuoles, the endoplasmic reticulum and the cell wall constitute storage compartments for mobilizable calcium. In contrast, the role of organelles surrounded by a double membrane (e.g. mitochondria, chloroplasts and nuclei) is more complex. Here, we review experimental data showing that t…

OrganellesEndoplasmic reticulumCell Membranechemistry.chemical_elementCell BiologyCell compartmentationCalciumBiologyPlantsCalcium in biologyDynamics of cytosolic and organelle calciumCell biologyCytosolCytosolchemistryCytoplasmOrganellePlant cell organizationCalciumCalcium SignalingMolecular BiologyCellular compartmentCalcium signalingPlant cell signalingBiochimica et biophysica acta
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Effect of calcium hydroxide dressing on microleakage of composite restorations in endodontically treated teeth subsequent to bleaching

2009

Objective: This study evaluates the effect of calcium hydroxide dressing on microleakage of composite restorations following non-vital bleaching. Methods: A total of 45 sound extracted human maxillary central incisors underwent endodontic treatment. The teeth were randomly divided into three groups (n=15). In group 1, access cavities were restored with composite. In group 2, the teeth underwent a bleaching procedure for one week before being restored with composite. In group 3, following a bleaching procedure, calcium hydroxide paste was placed in the pulp chamber for one week. The teeth were then restored with composite. The specimens were subjected to a dye leakage test. The data was anal…

OrthodonticsDental LeakageTooth NonvitalCalcium hydroxideDye leakagebusiness.industryComposite numberDentistry:CIENCIAS MÉDICAS [UNESCO]BandagesCalcium Hydroxidechemistry.chemical_compoundOtorhinolaryngologychemistryUNESCO::CIENCIAS MÉDICASTooth BleachingHumansSurgeryMaxillary central incisorbusinessGeneral Dentistry
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