Search results for "Calco"
showing 10 items of 53 documents
El yacimiento calcolítico de Karea en el contexto de las cuevas sepulcrales de Gipuzkoa (País Vasco)
2018
En el yacimiento arqueológico de Karea (Aia, Gipuzkoa), integrado por las cavidades contiguas de Karea-A y Karea-B, se recuperaron numerosos restos cerámicos y faunísticos (Karea-A) y vestigios de una inhumación calcolítica (Karea-B). El presente trabajo se ha vertebrado en dos ejes. En primer lugar, el estudio de ambos depósitos mediante diferentes disciplinas: (1) estudio antropológico; (2) estudio del ajuar, compuesto por restos cerámicos y faunísticos; (3) identificación antracológica de los restos leñosos recuperados en el depósito funerario y por último, (4) estudios isotópicos que nos han permitido obtener datos de la alimentación (δ13C y δ15N) y procedencia (87Sr/86Sr) del sujeto in…
Captación y selección de materias primas en la primera metalurgia del Sureste de la península ibérica
2020
The authors are grateful for the technical and human support provided by SGIker of UPV/EHU and European funding (ERDF and ESF). We are also in debt with Eduardo Galán, Ruth Maicas and Carmen Cacho, curators of the Museo Arqueológico Nacional (Madrid) for facilitating the sampling and study of metal objects as well as with Ignacio Soriano Llopis for his help on the selection and sampling of the Palmela points, with Massimo Chiaradia who performed the analyses of the Palmela points at the Department of Earth Sciences (University of Geneva, Switzerland) and with Óscar García Vuelta for his pictures of some assemblages. We also appreciate and the careful work of editing and style of the TP edit…
TheGCA1gene encodes a glycosidase-like protein in the cell wall ofCandida albicans
2016
Candida albicans Gca1p is a putative glucoamylase enzyme which contains 946 amino acids, 11 putative sites for N -glycosylation and 9 for O -glycosylation. Gca1p was identified in β-mercaptoethanol extracts from isolated cell walls of strain C. albicans SC5314 and it is involved in carbohydrate metabolism. The significance and the role of this protein within the cell wall structure were studied in the corresponding mutants. The homozygous mutant showed that GCA1 was not an essential gene for cell viability. Subsequent phenotypic analysis performed in the mutants obtained did not show significant difference in the behavior of mutant when compared with the wild strain SC5314. Zymoliase, Calco…
un approccio teorico-sperimentale per la ricostruzione del profilo trasversale di rotaie usurate
2012
La sovrastruttura ferroviaria durante la sua vita utile è sottoposta oltre ai carichi dinamici generati dai rotabili (azioni verticali, laterali, ecc.), anche ai carichi termici e ad azioni ambientali di varia natura. Tali sollecitazioni, interagendo sinergicamente tra loro, determinano il deterioramento della sovrastruttura ferroviaria inteso come discostamento dalla configurazione geometrica di progetto e l’usura dei singoli componenti del binario e della massicciata. Per valutare lo stato di efficienza della sovrastruttura è consuetudine far riferimento ad alcuni parametri geometrici del binario che, peraltro, permettono di formulare un giudizio oggettivo circa il livello di sicurezza of…
Pga13 in Candida albicans is localized in the cell wall and influences cell surface properties, morphogenesis and virulence.
2011
The fungal cell wall is an essential organelle required for maintaining cell integrity and also plays an important role in the primary interactions between pathogenic fungi and their hosts. PGA13 encodes a GPI protein in the human pathogen Candida albicans, which is highly up-regulated during cell wall regeneration in protoplasts. The Pga13 protein contains a unique tandem repeat, which is present five times and is characterized by conserved spacing between the four cysteine residues. Furthermore, the mature protein contains 38% serine and threonine residues, and therefore probably is a highly glycosylated cell wall protein. Consistent with this, a chimeric Pga13-V5 protein could be localiz…
Identification of a mannoprotein present in the inner layer of the cell wall of Saccharomyces cerevisiae.
1997
Cell wall extracts from the double-mutant mnn1 mnn9 strain were used as the immunogen to obtain a monoclonal antibody (MAb), SAC A6, that recognizes a specific mannoprotein--which we have named Icwp--in the walls of cells of Saccharomyces cerevisiae. Icwp runs as a polydisperse band of over 180 kDa in sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of Zymolyase extracts of cell walls, although an analysis of the secretory pattern of the mannoprotein shows that at the level of secretory vesicles, it behaves like a discrete band of 140 kDa. Immunofluorescence analysis with the MAb showed that Icwp lies at the inner layer of the cell wall, being accessible to the antibody on…
A funerary perspective on the Bell Beaker period in the Wester Mediterranean Reading the social context of individual burials at La Vital (Gandia, Va…
2013
[EN] The discussion about social dynamics in recent Pre- history is supported by the study of funerary practices. The presence and significance of individual and collec- tive burials in Final Neolithic/Chalcolithic societies in the southeast of the Iberian Peninsula provides new evi- dence for the debate concerning the emergence of social inequalities in this region. Our contribution is based on the recent discovery and excavation of several individual pit burials in domestic contexts at the site of La Vital (Gandía, Valencia). The particularities of the identified burials (different ritual episodes) and their content (Bell Beaker pottery, metal objects, animal offerings) together with an …
Enterramientos prehistóricos en fosa en el entorno megalítico de Los Molares (margen izquierda de la cuenca del Bajo Guadalquivir)
2020
El artículo presenta nuevos datos procedentes de excavaciones recientes en Los Molares (provincia de Sevilla), en el entorno próximo de los dólmenes de El Palomar y Cañada Real. Los datos fueron obtenidos durante los trabajos arqueológicos previos a la urbanización de la finca de El Palomar, localizándose varias fosas rellenas con materiales prehistóricos, tres de ellas con restos humanos. Se presentan sus contextos arqueológicos y restos recuperados, así como los resultados de los análisis radiocarbónicos efectuados. Se realiza una discusión sobre las implicaciones de estos nuevos hallazgos en relación con el entorno megalítico conocido y con el registro funerario del IV milenio cal BC en …
Characterization of a disulphide-bound Pir-cell wall protein (Pir-CWP) ofYarrowia lipolytica
2003
In this work we have studied the disulphide-bound group of cell wall mannoproteins of Yarrowia lipolytica and Candida albicans. In the case of Y. lipolytica, SDS-PAGE analysis of the beta-mercaptoethanol-extracted material from the purified cell walls of the yeast form, showed the presence of a main polypeptide of 45 kDa and some minor bands in the 100-200 kDa range. This pattern of bands is similar to that obtained in identical extracts in Saccharomyces cerevisiae (Moukadiri et al., 1999), and besides, all these bands cross-react with an antibody raised against beta-mercaptoethanol-extracted material from the purified cell walls of S. cerevisiae, suggesting that the 45 kDa band could be th…
Metodo speditivo per il dimensionamento di ali gocciolanti in pendenza
2015
Metodo innovativo particolarmente semplice (solo due equazioni monomie) che consente di dimensionare ali gocciolanti comunque pendenti lungo i filari irrigui, massimizzando al tempo stesso il risparmio energetico (Baiamonte, 2018a). Il metodo pertanto sostituisce quello tradizionale basato su numerosi tentativi di calcolo di natura iterativa.