Search results for "Callu"

Micropropagation and in vitro culture of Pyrethrum [Chrysanthemum cinerariifolium (Trev.) Vis.]

2014

Pyrethrum (Chrysanthemum cinerariifolium (Trev.) Vis. = Tanacetum cineariaefolium (Trev.) Schultz-Bip.) is a perennial herbaceous plant belonging to the family Asteraceae, native to Albania and the area of former Yugoslavia. It is the only species in the genus Tanacetum having an agronomic importance, although the genus consists of several species producing similar types of bioactive metabolites. The species is grown in order to obtain the insecticidal compounds collectively termed pyrethrins, which are found primarily in the flower heads. In this work we discuss the results found from a worldwide literature review about the micropropagation techniques followed on Pyrethrum, the in vitro cu…

Biotechnology Applications of Plant Callus Cultures

2019

In ethnopharmacology, and especially in traditional Chinese medicine, medicinal plants have been used for thousands of years. Similarly, agricultural plants have been used throughout the history of mankind. The recent development of the genetic engineering of plants to produce plants with desirable features adds a new and growing dimension to humanity’s usage of plants. The biotechnology of plants has come of age and a plethora of bioengineering applications in this context have been delineated during the past few decades. Callus cultures and suspension cell cultures offer a wide range of usages in pharmacology and pharmacy (including Chinese medicine), as well as in agriculture and horticu…

Über den Allantoinstoffwechsel des Laubmooskallus und seine Beeinflussung durch Licht

1972

Summary 1. The amount of allantoin and allantoate was determined in moss callus grown under various light conditions. It can reach 26,5 % of the plant nitrogen content. The accumulation is influenced by light intensity and colour. 2. Red-light (660 nm) causes a greater allantoin level in the cells than blue light and white light illumination. Red-light favours the formation of young setae from the moss callus. We could detect a relation between the intensity of seta-development and the amount of allantoin. The accumulation is increasing with the growth of the setae. 3. Blue-light (440 nm) enhances the chlorophyll synthesis and promotes chloroplast develop ment. This effect is correlated wit…

Somatic embryogenesis and plant regeneration from pistil transverse thin cell layers of lemon (Citrus limon)

2008

Callus induction, somatic embryogenesis and plant regeneration were obtained in Citrus limon (L.) Burm. (cv. Femminello) from cultures of pistil transverse thin cell layer explants [(t)TCLs]. Explants were cultured on two different media, based on Murashige and Skoog salts and vitamins, supplemented with 500 mg l-1 malt extract (MSI), or 500 mg l-1 malt extract and 13.3 ¼M 6-benzylaminopurine (MSII). Sucrose (146 mM) was used as carbon source. Somatic embryos appeared 3 months after culture initiation from stigma and style (t)TCLs; they were observed at the surface of the (t)TCL-derived callus. Although ovary (t)TCLs showed the highest callus formation, they never differentiated somatic emb…

Rice protoplasts isolated directly from mature-embryo-derived calli

1996

Mature-embryo-derived calli of japonica rice (Oryza sativa L) Taipei 309 were used for replicated protoplast isolation experiments. Six out of nine callus lines produced protoplasts with satisfactory yield of 5.20×106–8.96×106 protoplasts/g FW (fresh weight). The remaining three callus lines initiated from seeds of cryopreserved-callus-derived plants had rooty calli, resulting in low yield of protoplasts and a large number of isolated banana-shape intact cells. Viability of protoplasts ranged 87.46%–94.15%. The average size of protoplasts was 207.49–379.04 μm2 in different callus lines. Comparitive experiments were also carried out using both calli and suspension culture cells for protoplas…

Osteogenesis imperfecta and hyperplastic callus formation: light- and electron-microscopic findings.

1993

In rare cases of osteogenesis imperfecta, an "overshoot" growth of new bone may occur, which, clinically gives the impression of a tumour. This condition is known as hyperplastic callus formation. Morphology showed an excessive mixed desmal-chondral osteoneogenesis. Atypical collagen fibrils in non-callus tissue represent an indicator for this callus formation in individual patients.

Monitoring of fracture calluses with color Doppler sonography.

1999

Purpose Fracture callus formation is closely associated with vascular invasion, and the use of color Doppler sonography has been suggested as a means to monitor, earlier than gray-scale sonography, the first stages of the healing process. We report the findings in a series of patients with tibial fractures in whom both gray-scale sonography and color Doppler imaging were employed to monitor new bone formation at the fracture site. Methods Twenty patients with tibial fractures treated with external fixator frames were examined sonographically about 10 days after surgery and then about every 25 days until radiographic demonstration of consolidation. Results Eighteen of 20 patients had a well-…

Influence of SiO2 nanoparticles on relative fluorescence of plant cells

2015

Nanoparticles (nano-scale particles (NSPs)) are defined as particles with dimensions less than 100 nm. SiO<sub>2</sub> nanoparticles are one of the most widely common nanoparticles in the environment, particularly in urban areas. The sources of SiO<sub>2 </sub>nanoparticles are very different, including natural nanoparticles, anthropogenic and engineered nanoparticles. The SiO<sub>2 </sub>nanoparticles could be considered a source of different pollution effects on leaving organisms. Nevertheless, knowledge of the mechanisms, through which the SiO<sub>2 </sub>nanoparticles affect cells, is incomplete. The aim of the research was to elaborate a …

Healing in Denervated Bones

1974

Effects of systemically transplanted allogeneic bone marrow multipotent mesenchymal stromal cells on rats' recovery after experimental polytrauma.

2013

Background The aim of this study was to evaluate impact of transplantation of bone marrow mesenchymal stromal cells (BM MMSCs) on recovery after polytrauma and bone fracture repair. Methods A total 27 Wistar-Kyoto rats were divided into three groups (n = 9): normal control (A), polytrauma (B), and polytrauma treated with BM MMSC transplantation (C). The experimental polytrauma model was made on male rats by causing multiple fractures and hemorrhagic shock. At 36 hours 9 days after surgery, nine rats received allogeneic BM MMSCs (1 × 10(6) cells per kilogram) intravenously. The day before operation and at Days 3 and 10 after surgery as well as at the end of the experiment, blood analysis was…