Search results for "Callus formation"
showing 3 items of 13 documents
Stimulation of root and somatic embryo production in Euconymus europaeus L. by an inhibitor of polyamine biosynthesis
1995
In vitro formation of roots and somatic embryos is obtained from cotyledon explants of a Spindle tree (Euonymus europaeus L.) cultured on two different media: a medium inducing callus formation and the production of roots, and a medium inducing callus formation, root and somatic embryo production. We studied the effects of α-difluoromethylornithine (DFMO), a specific, irreversible inhibitor of ornithine decarboxylase (ODC) on root and somatic embryo production, growth and titers of putrescine in Euonymus explants and explant-derived calli. Early changes in putrescine levels were detected in both cultures before the visible emergence of roots or somatic embryos. DFMO rapidly inhibited putres…
Spermidine and morphogenesis in single cell cultures of Sideritis angustifolia lag.
1990
Abstract Single cells from hypocotyl-derived callus of Sideritis angustifolia were evaluated for morphogenesis when cultured in either solidifier or liquid Murashige and Skoog medium supplemented with various concentrations of spermidine, naphthaeneacetic acid (NAA), benzyladenine (BA) and dicyclohexylamine (DCHA). Spermidine (Spd) did not replace the hormonal requirements for cell division and callus formation, but affected growth induced by NAA or BA. This polyamine increased the plating efficiency of cells cultured in the presence of NAA (0.05, 0.54 and 5.40 μM) or BA (0.40, 4.40 and 8.80 μM). In contrast, Spd reduced growth induced by 10.80 μM NAA, optimal auxin concentration for callus…
Hyperplastic callus formation in osteogenesis imperfecta: CT and MRI findings.
1998
Hyperplastic callus formation is a noteworthy condition in patients with osteogenesis imperfecta because it often mimicks osteosarcoma on radiography. The findings of CT and MRI in hyperplastic callus formation have not been reported. In the presented case, MRI demonstrated contrast enhancement and edema of the surrounding soft tisssue, consistent with benign as well as malignant disease. Computed tomography showed a calcified rim of the lesion which may be a useful feature to rule out osteosarcoma in this condition.