Search results for "Cases"

showing 10 items of 124 documents

Insights into mRNP biogenesis provided by new genetic interactions among export and transcription factors.

2012

Abstract Background The various steps of mRNP biogenesis (transcription, processing and export) are interconnected. It has been shown that the transcription machinery plays a pivotal role in mRNP assembly, since several mRNA export factors are recruited during transcription and physically interact with components of the transcription machinery. Although the shuttling DEAD-box protein Dbp5p is concentrated on the cytoplasmic fibrils of the NPC, previous studies demonstrated that it interacts physically and genetically with factors involved in transcription initiation. Results We investigated the effect of mutations affecting various components of the transcription initiation apparatus on the…

Nucleocytoplasmic Transport ProteinsSaccharomyces cerevisiae Proteinslcsh:QH426-470MutantActive Transport Cell NucleusRNA-binding proteinRNA polymerase IISaccharomyces cerevisiaeDEAD-box RNA HelicasesTranscription (biology)GeneticsGenetics(clinical)RNA MessengerNuclear poreMex67pTranscription factorGenetics (clinical)AllelesDbp5pGeneticsmRNA exportbiologyGeneral transcription factorfungiNuclear ProteinsRNA-Binding Proteinslcsh:GeneticsRibonucleoproteinsMutationbiology.proteinNuclear PoreRNA Polymerase IINuclear Pore ComplexTranscriptionBiogenesisTranscription FactorsResearch ArticleBMC genetics
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Cultural institutions as agents of urban and community regeneration in the (post-)pandemic city. The case of the «Laboratorio Zen Insieme» in Palermo

2022

Although all cities in the world have been affected by the COVID-19 pandemic, its impacts on the territories, yet to be understood, are unevenly distributed, revealing extremely varied imbalances depending on the places. However, it is clear that the virus and its variants have aggravated pre-existing socio-spatial inequalities, creating new ones and bringing attention back to those implications between space, planning, public health and citizenship that are at the origins of contemporary urbanism. In a reference framework in which the crisis is globalized but unequal and in the absence of a welfare system capable of responding to the urgencies of the most marginalized social contexts and g…

Olsen 2018Settore ICAR/21 - UrbanisticaSettore M-GGR/01 - GeografiaSacco and Blessi 2009). In the current (post-) pandemic context and through the lens of a southern European perspective the purpose of this article is to critically reflect about the role of culture as possible vehicle of urban and community regeneration. In particular we will focus on the activities of the no profit organization «Laboratorio Zen Insieme» in ZEN2 one of the last large popular and peripheral neighborhoods built in Palermo at the end of 80s in order to explore and understand how cultural practices work as agent of urban and social transformation capable of addressing emerging issues especially in the pandemic scenario we are experiencing. Thecasestudy has been conducted through analysis of documents participative observations (Honer and Hitzler 2015) and qualitative in-depth interviews with key actors involved in the conception organization and management of the activities carried out by Laboratorio Zen Insieme with representatives of local institutions and non-formal conversations with participants of the workshops heldin the neighborhood. The experience we narrate finds that cultural practices have re-conceptualized their design and functions as strategies of urban and community regeneration and at the same time have contributed to answer to emergent issues in developing proximity and local based strategies facing up to problems inherent civil rights educationalpoverty socio-spatial justice and have changed the image and identity of urban places they inhabit.In this sense the research provides a framework for development of strategies and legitimization for cultural practices and a point of discussionabouttheirrolein urban development.Although all cities in the world have been affected by the COVID-19 pandemic its impacts on the territories yet to be understood are unevenly distributed revealing extremely varied imbalances depending on the places. However it is clear that the virus and its variants have aggravated pre-existing socio-spatial inequalities creating new ones and bringing attention back to those implications between space planning public health and citizenship that are at the origins of contemporary urbanism. In a reference framework in which the crisis is globalized but unequal and in the absence of a welfare system capable of responding to the urgencies of the most marginalized social contexts and groups a response to the new social and individual needs has been offered by cultural institutions that play a role of territorial agency often independently or in the absence of political institutions. Far from the idea of entertainment and divertissement it is in fact increasingly clear how the practices of cultural innovation experimenting with various forms of action and participation can in some cases play a fundamental role in the processes of social cohesion and community building representing an antidote to the worsening of the phenomena of marginalization and socio-spatial inequalities within cities and territories (Colantonio and Dixon 2011
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The taint of torture and the brazilian legal system

2019

In Brazil, the practice of torture intertwines deeply with its own history. The literature on the subject has had a greater intensification on its registers during the Dictatorial Regime (1964 to 1985). In spite of the Brazilian Constitution of 1988, Brazil only makes efforts to investigate the crimes of torture of this period to comply with the provisions of the United Nations in 2011. Within this scenario, the NationalTruth Commission (Law 12.528, of November 2011) was created with the purpose of clarifying the facts and circumstances of cases concerning human rights violations, as well as the Law on Access to Information (Law 12.527 of November 2011), which have regulated the constitutio…

Orden legal 37 491137-7038 8537 Arxius de sociologia 525290 2019 40 7038138 The taint of torture and the brazilian legal system De Góes BrennandEdna GusmãoTortureas well as the Law on Access to Information (Law 12.527 of November 2011)of November 2011) was created with the purpose of clarifying the facts and circumstances of cases concerning human rights violationssuch as tortureespecially because they were perpetrated by agents of the State. This article aims to present part of the research undertaken to understand the official legal procedures to prevent the practice of acts of torture in the national territory and how they are understood in the Brazilian legal systemUNESCO::SOCIOLOGÍADelamar José In Brazilwas created the Network Studies and Research on Violence-RIEV in partnership with Federal University of Paraíba-UFPB and Federal University of Santa Catarina-UFSC with the objective of investigating violations of human rights during the dictatorship1997 that criminalizes torture. ViolenceLegal orderthe practice of torture intertwines deeply with its own history. The literature on the subject has had a greater intensification on its registers during the Dictatorial Regime (1964 to 1985). In spite of the Brazilian Constitution of 1988Brazil only makes efforts to investigate the crimes of torture of this period to comply with the provisions of the United Nations in 2011. Within this scenariowhich have regulated the constitutional right to access public informationVolpato Dutrawith special attention to Law 9.455:SOCIOLOGÍA [UNESCO]the NationalTruth Commission (Law 12.528of April 7ViolenciaTorturavalid for all areas of the public administration. These laws allowed the opening of the archives of the dictatorship until denied on the basis of a severely restricted access. In this contextpersecution and violations of the right-to-live. The study of these violations is of fundamental importance for the historical unveiling of this period
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On the Very Idea of a Robust Alternative

2011

According to the Principle of Alternative Possibilities (PAP), an agent is morally responsible for an action of hers only if she could have done otherwise. The notion of a robust alternative plays a prominent role in recent attacks on PAP based on so-called Frankfurt cases. In this paper I defend the truth of PAP for blameworthy actions against Frankfurt cases recently proposed by Derk Pereboom and David Widerker. My defence rests on some intuitively plausible principles that yield a new understanding of the concept of a robust alternative. I will leave aside whether PAP also holds for praiseworthy actions.

PhilosophyAction (philosophy)AsideIf and only ifPhilosophyMoral responsibilityFrankfurt casesEpistemologyCrítica (México D. F. En línea)
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Doing One’s Best, Alternative Possibilities, and Blameworthiness

2014

En este trabajo me propongo principalmente mejorar y dar un apoyo mas firme a una defensa del Principio de Posibilidades Alternativas (PPA) frente a los casos Frankfurt que propuse en trabajos anteriores. En el presente articulo concentro mi atencion en un reciente y famoso caso Frankfurt, �Evasion de impuestos�, disenado por Derk Pereboom. Tras presentar los aspectos esenciales de mi defensa del PPA y aplicarlos al caso de Pereboom, considero distintas objeciones que han sido (o podrian ser) planteadas a dicha defensa y sostengo que no tienen exito. Concluyo senalando que mi critica al ejemplo de Pereboom sugiere una estrategia general contra otros casos Frankfurt, tanto posibles como efec…

PhilosophyInjury controlAccident preventionPhilosophyPoison controlFrankfurt casesHumanitiesCrítica (México D. F. En línea)
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Complex formation between the NS3 serine-type proteinase of the hepatitis C virus and NS4A and its importance for polyprotein maturation

1995

Processing of the hepatitis C virus polyprotein is mediated by host cell signalases and at least two virally encoded proteinases. Of these, the serine-type proteinase encompassing the amino-terminal one-third of NS3 is responsible for cleavage at the four sites carboxy terminal of NS3. The activity of this proteinase is modulated by NS4A, a 54-amino-acid polyprotein cleavage product essential for processing at the NS3/4A, NS4A/4B, and NS4B/5A sites and enhancing cleavage efficiency between NS5A and NS5B. Using the vaccinia virus-T7 hybrid system to express hepatitis C virus polypeptides in BHK-21 cells, we studied the role of NS4A in proteinase activation. We found that the NS3 proteinase a…

Protein ConformationRecombinant Fusion ProteinsvirusesGenetic VectorsMolecular Sequence DataImmunologyVaccinia virusHepacivirusProtein Sorting SignalsViral Nonstructural ProteinsBiologyKidneyTransfectionCleavage (embryo)MicrobiologyAntibodiesCell LineSerineEpitopesViral Proteinschemistry.chemical_compoundProtein structureProteinase 3CricetinaeVirologyAnimalsAmino Acid SequenceProtein PrecursorsNS5BPeptide sequenceNS3Sequence Homology Amino AcidSerine Endopeptidasesvirus diseasesbiochemical phenomena metabolism and nutritiondigestive system diseasesNS2-3 proteaseBiochemistrychemistryInsect ScienceProtein Processing Post-TranslationalAlgorithmsRNA HelicasesResearch ArticleJournal of Virology
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Childhood trauma and substance use underlying psychosis: a systematic review

2020

[EN] Background: Schizophrenia spectrum disorders (SSD) are mental diseases caused by a combination of genetic susceptibility and a number of environmental factors. Among these factors, the role of traumatic events suffered in childhood, as well as that of substance use, have been of particular research interest. Objectives: To conduct a systematic review to clarify whether there is an interaction between childhood trauma and substance use related to the diagnosis or symptoms of SSD. It was also the objective of this review to collate the associations that may exist between the three variables of the study (trauma, substance use and psychosis). Methods: We conducted a systematic search resu…

PsychosisSubstances useRC435-571Poison controlEsquizofreniaReview Article药物滥用Cannabis useSuicide preventionOccupational safety and healthChildhood traumaConsumo de cannabis思觉失调Injury preventionmedicinePsicosisPsychiatryWindow of opportunitybusiness.industryHuman factors and ergonomicsConsumo de sustanciasmedicine.diseasePsychosis• Schizophrenia is caused by a combination of genetic susceptibility and a number of environmental factors. Traumatic events suffered in childhood as well as substance use have been of particular interest.• Our results reveal a positive association between traumatic experiences in childhood and drug use and their interaction with schizophrenia spectrum disorders.• Detecting cases of childhood trauma as well as cases of trauma associated with substance use could be useful for the primary prevention of some psychiatric diseases such as psychosis.大麻使用精神分裂症SchizophreniaTrauma infantilSchizophreniabusiness童年创伤Clinical psychology
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Human germ cell differentiation from fetal- and adult-derived induced pluripotent stem cells

2010

Historically, our understanding of molecular genetic aspects of human germ cell development has been limited, at least in part due to inaccessibility of early stages of human development to experimentation. However, the derivation of pluripotent stem cells may provide the necessary human genetic system to study germ cell development. In this study, we compared the potential of human induced pluripotent stem cells (iPSCs), derived from adult and fetal somatic cells to form primordial and meiotic germ cells, relative to human embryonic stem cells. We found that ∼5% of human iPSCs differentiated to primordial germ cells (PGCs) following induction with bone morphogenetic proteins. Furthermore, …

Recombinant Fusion ProteinsInduced Pluripotent Stem CellsEmbryoid bodyHaploidyBiologyCell LineDEAD-box RNA Helicases03 medical and health sciences0302 clinical medicineGeneticsmedicineHumansInduced pluripotent stem cellMolecular BiologyEmbryonic Stem CellsGenetics (clinical)030304 developmental biology0303 health sciences030219 obstetrics & reproductive medicineSynaptonemal ComplexGene Expression ProfilingGene Expression Regulation DevelopmentalRNA-Binding ProteinsCell DifferentiationArticlesGeneral MedicineEmbryonic stem cellMolecular biologyGerm Cellsmedicine.anatomical_structureBone Morphogenetic ProteinsGerm line developmentStem cellReprogrammingGerm cellAdult stem cellHuman Molecular Genetics
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Centenarians maintain miRNA biogenesis pathway while it is impaired in octogenarians.

2016

Centenarians but not octogenarians up regulate the expression of miRNAs, as we previously reported. We have looked into miRNA biogenesis. We show that RNA POL II, DROSHA, EXPORTIN 5 and DICER, are up-regulated in centenarians compared with octogenarians. Furthermore, factors involved in the control of these miRNAs biogenesis genes are also up-regulated in centenarians. Therefore, the up-regulation of miRNA expression in centenarians can be explained in part because miRNA biogenesis pathway is depressed in octogenarians (ordinary aging) while it is maintained in centenarians (extraordinary aging).

Ribonuclease III0301 basic medicineAgingmedia_common.quotation_subjectRNA polymerase IIKaryopherinsBioinformaticsDEAD-box RNA Helicases03 medical and health sciencesmicroRNAHumansGeneDroshamedia_commonAged 80 and overGeneticsbiologyAge FactorsLongevityUp-RegulationMicroRNAs030104 developmental biologybiology.proteinRNA Polymerase IITranscriptomeMiRNA biogenesisBiogenesisDevelopmental BiologyDicer
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DICER and ZRF1 contribute to chromatin decondensation during nucleotide excision repair

2016

Abstract Repair of damaged DNA relies on the recruitment of DNA repair factors in a well orchestrated manner. As a prerequisite, the chromatin needs to be decondensed by chromatin remodelers to allow for binding of repair factors and for DNA repair to occur. Recent studies have implicated members of the SWI/SNF and INO80 families as well as PARP1 in nucleotide excision repair (NER). In this study, we report that the endonuclease DICER is implicated in chromatin decondensation during NER. In response to UV irradiation, DICER is recruited to chromatin in a ZRF1-mediated manner. The H2A–ubiquitin binding protein ZRF1 and DICER together impact on the chromatin conformation via PARP1. Moreover, …

Ribonuclease III0301 basic medicineDNA RepairUltraviolet RaysDNA damageDNA repairgenetic processesPoly (ADP-Ribose) Polymerase-1Genome Integrity Repair and ReplicationBiologyChromatin remodelingCell LineDEAD-box RNA HelicasesHistones03 medical and health scienceschemistry.chemical_compoundUbiquitinCell Line TumorGeneticsAnimalsHumansCaenorhabditis elegansOncogene ProteinsOsteoblastsUbiquitinfungiRNA-Binding ProteinsFibroblastsChromatin Assembly and DisassemblyMolecular biologyChromatinChromatinDNA-Binding Proteinsenzymes and coenzymes (carbohydrates)HEK293 Cells030104 developmental biologychemistrybiology.proteinDNADNA DamageMolecular ChaperonesNucleotide excision repairDicerNucleic Acids Research
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