Search results for "Cellular"
showing 10 items of 6449 documents
Molecular mechanisms in developmental biology.
1996
Some general molecular mechanisms underlying development are described. Namely: those involved in the differentiation of the R7 receptor in Drosophila embryonic retina; those involved in the determination of embryonic axes and in polar cell differentiation, in Drosophila; those involved in the determination of the AB and P cell lineage and in vulva differentiation in Caenorhabditis embryos.
Pathogenicity of live bacteria and extracellular products of motileAeromonasisolated from eels
1995
The pathogenic activities in vitro and in vivo of live bacteria and extracellular products (ECP) of 24 motile Aeromonas strains were investigated. Most Aer. hydrophila and Aer. jandaei isolates were pathogenic for eels (LD50 105·4‐107·6 cfu fish‐1) but no Aer. sobria, Aer. caviae and Aer. allosaccharophila caused mortality in eels at doses of > 108·4 cfu fish‐1. Of these Aeromonas strains, Aer. hydrophila and Aer. jandaei in particular produced elastases and haemolysins against fish erythrocytes. ECP from Aer. hydrophila and Aer. jandaei caused degenerative changes in fish cell lines and were strongly toxic for eels (LD50 1·0–3·2 μg (g fish)‐1) reproducing the symptoms associated with natur…
Allele-specific silencing as therapy for familial amyotrophic lateral sclerosis caused by the p.G376D TARDBP mutation
2022
Abstract Amyotrophic lateral sclerosis is a neurodegenerative disease characterized by the degeneration of motor neurons. There is no treatment for this disease that affects the ability to move, eat, speak and finally breathe, causing death. In an Italian family, a heterozygous pathogenic missense variant has been previously discovered in Exon 6 of the gene TARDBP encoding the TAR DNA-binding protein 43 protein. Here, we developed a potential therapeutic tool based on allele-specific small interfering RNAs for familial amyotrophic lateral sclerosis with the heterozygous missense mutation c.1127G>A. We designed a small interfering RNA that was able to diminish specifically the express…
Studio dell'apoptosi cellulare nella pre-eclampsia mediante valutazione del CD95 linfocitario in pazienti trattate con antitrombina III
2009
L'etimologia della pre-eclampsia rimane ancora oggi sconosciuta ma sappiamo abbastanza sulla sua fisiopatologia, da consentire l'adozione di presidi terapeutici atti ad espletare un controllo sintomatologico della sindrome ma non a curarla. Nel presente lavoro ci siamo posti l'obiettivo di valutare in corso di trattamento con ATIII l'andamento dei valori di CD95 dei linfociti del circolo periferico, in qualità di indicatore dei processi di apoptosi cellulare in una condizione di "Graft versus host disease", quale potrebbe considerarsi la gravidanza complicata da pre-eclampsia.
Potential roles of extracellular vesicles in brain cell-to-cell communication
Potential roles of extracellular vesicles in brain cell-to-cell communication Extracellular vesicles (EVs) are released into thè extracellular space from both cancer and normal brain cells, and are probably able to modify thè phenotypic properties of receiving cells1. EVs released from astrocytes and neurons contain FGF2 and VEGF2'3 and induce a 'blood-brain barrier' (BBB) phenotype in cultured brain capillary endothelial cells (BCECs, unpublished results), On thè other hand, EVs from G26/24 oligodendroglioma induce apoptosis in neurons and astrocytes4-5. These effects are probably due to Fas Ligand and TRAIL, present in G26/24 vesicles4-5. Moreover, G26/24 EVs contain extracellular matrix …
Autophagy
2012
Klionsky, Daniel J. et al.
Glutathione in Cancer Cell Death
2011
Glutathione (L-γ-glutamyl-L-cysteinyl-glycine; GSH) in cancer cells is particularly relevant in the regulation of carcinogenic mechanisms; sensitivity against cytotoxic drugs, ionizing radiations, and some cytokines; DNA synthesis; and cell proliferation and death. The intracellular thiol redox state (controlled by GSH) is one of the endogenous effectors involved in regulating the mitochondrial permeability transition pore complex and, in consequence, thiol oxidation can be a causal factor in the mitochondrion-based mechanism that leads to cell death. Nevertheless GSH depletion is a common feature not only of apoptosis but also of other types of cell death. Indeed rates of GSH synthesis and…
Novel Insights into the Cellular Localization and Regulation of the Autophagosomal Proteins LC3A, LC3B and LC3C
2020
Macroautophagy is a conserved degradative process for maintaining cellular homeostasis and plays a key role in aging and various human disorders. The microtubule-associated protein 1A/1B light chain 3B (MAP1LC3B or LC3B) is commonly analyzed as a key marker for autophagosomes and as a proxy for autophagic flux. Three paralogues of the LC3 gene exist in humans: LC3A, LC3B and LC3C. The molecular function, regulation and cellular localization of LC3A and LC3C have not been investigated frequently, even if a similar function to that described for LC3B appears likely. Here, we have selectively decapacitated LC3B by three separate strategies in primary human fibroblasts and analyzed the evoked e…
Borrelia burgdorferi Outer Membrane Vesicles Contain Antigenic Proteins, but Do Not Induce Cell Death in Human Cells
2022
Like many bacterial species, Borrelia burgdorferi, the pleomorphic bacterium that causes Lyme borreliosis, produces outer membrane vesicles (OMVs). Borrelial OMVs (BbOMVs) have been identified as containing virulence factors, such as outer surface proteins (Osps) A, B, and C, as well as DNA. However, the pathogenicity of BbOMVs in disease development is still unclear. In this study, we characterized purified BbOMVs by analyzing their size and immunolabeling for known antigenic markers: OspA, OspC, p39, and peptidoglycan. In addition, BbOMVs were cocultured with human non-immune cells for cytotoxicity analysis. The results demonstrated that, on average, the vesicles were small, ranging betwe…
Ekstracelulārās vezikulās ietvertās mikrobiālās RNS atklāšana cilvēku asins un urīna paraugos
2020
Gandrīz visi cilvēka šūnu tipi sekretē ekstracelulārās vezikulas (EVs), un tām ir svarīga loma starpšūnu komunikācijā. Jaunākie pētījumi liecina, ka komunikācija notiek arī starp cilvēka mikrobiotu un saimniekorganisma šūnām. Šajā pētījumā mēs parādām, ka no cilvēka asinīm un urīna izdalītās EVs satur RNS fragmentus no dažādiem mikroorganismiem. Visbiežāk sastopami baktēriju tipi un supertipi asinīs un urīnā bija Proteobacteria, PVC grupa un Terrabacteria. Lai noteiktu vai šo mikrobiālo transkriptu galvenais avots ir zarnu mikrobioms, mēs izstrādājām EV izdalīšanas metodi no cilvēku fēču paraugiem. Tālāk mēs plānojām veikt RNS sekvencēšanas (RNA-seq) analīzi EV, kas izdalītas no plazmas, ur…