Search results for "Centro"
showing 10 items of 643 documents
Il recupero del Centro Storico di Gangi. Storia, piani e regole.
2005
Saggio sul recupero del centro storico di Gangi e il sistema delle connessioni e degli spazi aperti
Acquisition of thermotolerance in sea urchin embryos correlates with the synthesis and age of the heat shock proteins.
1986
Preheating at 31 degrees C induces thermotolerance in Paracentrotus lividus embryos, which therefore become able to withstand 1-h treatment at the otherwise lethal temperature of 35 degrees C, and to develop normally. The acquisition of thermotolerance is positively correlated with the amount of heat shock proteins produced during the 31 degrees C treatment. Evidence is provided that the heat shock proteins, although present in the embryo for long periods after synthesis, lose their effect on thermotolerance within 3 h of the cessation of synthesis.
Centrins, gatekeepers for the light-dependent translocation of transducin through the photoreceptor cell connecting cilium
2006
Centrins are members of a highly conserved subgroup of the EF-hand superfamily of Ca(2+)-binding proteins commonly associated with centrosome-related structures. In the retina, centrins are also prominent components of the photoreceptor cell ciliary apparatus. Centrin isoforms are differentially localized at the basal body and in the lumen of the connecting cilium. All molecular exchanges between the inner and outer segments occur through this narrow connecting cilium. Ca(2+)-activated centrin isoforms bind to the visual heterotrimeric G-protein transducin via an interaction with the betagamma-subunit. Ca(2+)-dependent assemblies of centrin/G-protein complexes may regulate the transducin mo…
DNA sequence and pattern of expression of the sea urchin (Paracentrotus lividus) alpha-tubulin genes.
1989
To study the molecular aspects of the regulation of transcription of a multigene family, we have isolated and sequenced cDNA and genomic clones coding for the alpha-tubulin of the sea urchin Paracentrotus lividus. Two cDNA clones, P alpha 10 and P alpha 4, contain respectively the coding information for 391 C-terminal and for 338 N-terminal amino acids of the 452 residues that constitute the complete protein. They show silent nucleotide substitutions only, suggesting that P alpha 10 and P alpha 4 represent the cloned copies of two allelic gene transcripts, which encode for two alpha-tubulin isoforms with identical amino acid sequence in the region of the overlap. The comparison of the predi…
¿Cómo deciden les jóvenes la transición a la educación postobligatoria? Diferencias entre centros públicos y privados-concertados
2017
Background of INCASI Project H2020-MSCA-RISE-2015 GA 691004. WP1: Compilation El objetivo del presente artículo es examinar el papel que juega la titularidad del centro en el que se cursa la educación secundaria obligatoria (ESO) en la decisión de transitar a la educación postobligatoria. En dicha encrucijada se contemplan también otras variables que se han constatado de gran peso en la toma de decisión como son las notas previas obtenidas, el nivel formativo familiar, el sexo, las expectativas y las aspiraciones, partiendo del modelo decisional de transiciones educativas[1] que contempla el modelo de Boudon sobre los efectos primarios y secundarios. Los resultados obtenidos son los derivad…
Tetrasomy 18p de novo: Identification by FISH with conventional and microdissection probes and analysis of parental origin and formation by short seq…
1996
We report a de novo supernumerary isochromosome 18p in a child with tetrasomy 18p, analyzed by a straightforward combination of cytogenetic and molecular cytogenetic methods. The diagnostic procedure consisted of standard banding techniques and fluorescence in situ hybridization (FISH) with centromere and library DNA probes for chromosome 18, and 18p-specific FISH probes prepared by chromosome microdissection and in vitro amplification. The maternal origin as well as the most probable cell stages of formation of the supernumerary isochromosome were determined by typing of short sequence repeats (SSRs). The pattern of allelic distribution suggests a nondisjunction during meiosis followed by …
Mapping of Friedreich's ataxia locus by identification of recombination events in patients homozygous by descent
1994
The Friedreich's ataxia locus (FRDA) maps on chromosome 9q13. Genetic data, obtained from a small number of recombination events, indicated that the FRDA locus might be located centromeric to the D9S15/D9S5 linkage group, the most probable order being cen-FRDA-D9S5-D9S111-D9S15-D9S110-qter. Recently, new centromeric markers have been reported. Analysis of these markers allowed us to localize the recombination breakpoint in some of the recombinant families. However, only one proximal recombination has been found with these markers. To increase the genetic information from FRDA families, we have analyzed the centromeric markers FR1, FR2, FR7, FR8, and FR5 in patients homozygous by descent. Th…
Pseudo-exclusion from paternity due to maternal uniparental disomy 16.
1998
The investigation of a case of disputed paternity revealed indirect exclusion of the alleged father in the haptoglobin system and in the DNA single-locus system D16S309/Hinf I (MS205). The paternity index for the non-exclusion systems was > 10(6). Since both exclusion systems (HP and MS205) are located on chromosome 16, we investigated 10 microsatellite loci covering this chromosome with 10-20 cM resolution. Analysis of the child's chromosome showed only alleles of maternal origin and lack of inheritance of paternal alleles for five informative loci. The markers close to the centromere of chromosome 16 were heterozygous, whereas distal loci were either heterozygous or homozygous for materna…
The evolution of human synteny 4 by mapping sub-chromosomal specific probes in Primates
2014
Comparative cytogenetic data concerning the orthologue to human chromosome 4 in primates shows that this chromosome is conserved between humans and non-human primates. However, the degree of conservation is not as high as previously estimated. In primates it is as a rule a large submetacentric chromosome but many exceptions are known especially in taxa characterized by a high level of chromosomal rearrangements. The rearrangements that have been visualized by chromosome painting so far, which are mostly interchromosomal changes, are in fact only a fraction of the actual chromosomal changes that have occurred during evolution. Intrachromosome changes can be analysed through classical cytogen…
Centromere-Like Elements in Megaselia Spiracularis (Diptera: Phoridae): A Fine-Structure and Cytogenetic Study
2004
The present study was concerned with the karyotype of a humpbacked fly, Megaselia spiracularis (Diptera: Phoridae). Chromosome preparations of neuroblasts isolated from male and female pupae uniformly showed two pairs of metacentric chromosomes and a telocentric chromosome pair. All chromosomes were homomorphic. The analysis of ultrathin serial sections through spermatogonia in metaphase using transmission electron microscopy, confirmed the presence of 3 regular chromosome pairs. In ultrathin sections, the centromeres could be detected as individual elements owing to their lesser electron density in comparison with the chromosome arms. In addition, the spindles contained two tiny elements s…