Search results for "Cholesterol"

A novel cholesterol-based detergent

2005

Design, synthesis and characterization of CHAPSTEROL, a novel cholesterol-based detergent developed for functional solubilization of cholesterol-dependent membrane proteins are described. To validate CHAPSTEROL, we employed the oxytocin receptor, a G protein-coupled receptor requiring cholesterol for its high-affinity binding state. Using the photoactivatable cholesterol analogue [3H]6,6-azocholestan-3beta-ol[3alphaH], we demonstrate that solubilization by CHAPSTEROL leads to an enrichment of cholesterol-binding proteins whereas the widely used bile acid derivative CHAPSO leads to a significant depletion of cholesterol-binding proteins. Similar to Triton X-100 and CHAPS, CHAPSTEROL maintain…

Lipid accumulation and metabolism in polychaete spermatogenesis: Role of the large discoidal lipoprotein

2010

In most oviparous animals, lipoprotein-mediated lipid transport plays an important role in the nutrient supply for the oocyte. In male gametes, lipids are used as energy substrates in spermatozoa but nothing is yet known about their origin and metabolism throughout spermatogenesis. The lipid profiles analyzed from different stages of male germ cell development in the marine annelid Nereis virens were found to undergo a dramatic change from primary triacylglycerides at the beginning of germ cell development to cholesterol and phospholipids at the end of development as demonstrated by HPLC with evaporative light scattering detection and mass spectrometry. The uptake of a large discoidal lipop…

Untersuchungen zur Ver�nderung des Gehaltes von Cholesterin, Gesamtlipid und Lipid-P im Serum von Ratten nach F�tterung von oxydierten Soja�len

1962

Characteristics of computed tomography angiography findings of coronary arteries in patients with suspected or established familial hypercholesterole…

2021

KOPSAVILKUMS Nosaukums: Koronāro artēriju datortomogrāfiskās angiogrāfijas atrades raksturojums pacientiem ar iespējamu vai konstatētu Ģimenes Hiperholesterinēmiju. Ievads: Ģimenes hiperholesterīnēmija ir biežākais iemesls pārmantotai dislipidēmijai visā pasaulē. Slimība attīstās nemanāmi, bet pēkšņi var kļūt fatāla. Tāpēc ir svarīgi to diagnosticēt vai izvērtēt bojājumu izteiktību cik agri iespējams, pirmsaterosklerotiskiem kardiovaskulāriem notikumiem.Sķiet, ka šobrīd CT angiogrāfija ir laba metode lai raksturotu izmaiņas koronārajās artērijās ĢH pacientiem. Mērkis: Raksturot aterosklerotiskās izmaiņas ĢH pacientu koronārajās artērijās izmantojot CT angiogrāfija rezultātus. Materiāls un m…

Ketogenic diet as antiepileptic therapy: Historical perspective

2017

Ketogenic diet (KD) is a high-lipid diet, adequate for protein content but low in carbohydrates content. Caloric intake is calculated on the basis of the ideal patient's needs, reduced by about 20%, and is about 90% of lipids present in 3: 1 or 4: 1 ratio with proteins and carbohydrates. KD is considered the most ancient antiepileptic therapy, actually proposed also for migraine therapy.

Ketogenic diet as antiepileptic therapy: Administration and formulas

2017

In 1921 Wilder defined classical cholesterol diet providing the patient with a daily calorie intake of less than 20% compared to age-related requirements. The main macronutrient is represented by saturated and long chain unsaturated fatty acids, in the ratio of 4: 1 to carbohydrates and proteins (4 grams of lipids per gram of carbohydrates and proteins). One of the first therapeutic protocols proposed is that of John Hopkins that the onset of the diet should take place in a hospital environment to assess the patient's response in a controlled situation.

Cholesterol level and hemorrhagic trasformation after ischemic stroke

2009

The Structure of Cholesterol in Lipid Rafts

2014

Rafts, or functional domains, are transient nano- or mesoscopic structures in the plasma membrane and are thought to be essential for many cellular processes such as signal transduction, adhesion, trafficking and lipid/protein sorting. Observations of these membrane heterogeneities have proven challenging, as they are thought to be both small and short-lived. With a combination of coarse-grained molecular dynamics simulations and neutron diffraction using deuterium labeled cholesterol molecules we observe raft-like structures and determine the ordering of the cholesterol molecules in binary cholesterol-containing lipid membranes. From coarse-grained computer simulations, heterogenous membra…

Cholesterol–Protein Interaction: Methods and Cholesterol Reporter Molecules

2010

Cholesterol is a major constituent of the plasma membrane in eukaryotic cells. It regulates the physical state of the phospholipid bilayer and is crucially involved in the formation of membrane microdomains. Cholesterol also affects the activity of several membrane proteins, and is the precursor for steroid hormones and bile acids. Here, methods are described that are used to explore the binding and/or interaction of proteins to cholesterol. For this purpose, a variety of cholesterol probes bearing radio-, spin-, photoaffinity- or fluorescent labels are currently available. Examples of proven cholesterol binding molecules are polyene compounds, cholesterol-dependent cytolysins, enzymes acce…

Impaired cellular cholesterol efflux by oxysterol-enriched high density lipoproteins.

1997

One of the proposed antiatherogenicity role of high-density lipoproteins (HDL) is believed to stimulate removal of cholesterol from the peripheral cells back to the liver for excretion. We have investigated the effects of oxidation-related modifications of HDL on their ability to stimulate cholesterol efflux from cultured cells. Human HDL (HDL3, 1.13 < d < 1.21 g/ml) have been modified either by malondialdehyde or by copper-mediated oxidation (Ox-HDL3). Compared with native HDL3, the modified HDL3 resulted in a significantly reduced efflux of labeled cholesterol from preloaded macrophages (P388D1 cell line). Analysis of lipid composition of Ox-HDL3 by gas chromatography revealed the presenc…