Search results for "Clearing"
showing 10 items of 19 documents
A survey of clearing techniques for 3D imaging of tissues with special reference to connective tissue
2016
AbstractFor 3-dimensional (3D) imaging of a tissue, 3 methodological steps are essential and their successful application depends on specific characteristics of the type of tissue. The steps are 1° clearing of the opaque tissue to render it transparent for microscopy, 2° fluorescence labeling of the tissues and 3° 3D imaging. In the past decades, new methodologies were introduced for the clearing steps with their specific advantages and disadvantages. Most clearing techniques have been applied to the central nervous system and other organs that contain relatively low amounts of connective tissue including extracellular matrix. However, tissues that contain large amounts of extracellular mat…
Reforming the international financial and monetary system: from Keynes to Davidson and Stiglitz
2004
International audience; With the usual debates trumpeting the usual merits of fixed versus flexible exchange rates, or some various incarnations thereof, the authors draw attention to Keynes's original plan for an international clearing union. The paper takes a close look at Joseph Stiglitz's recent suggestions to deal with financial crises along with Paul Davidson's Keynes-Post Keynesian clearinghouse. In the former case, the analysis is left wanting: the author offers nothing more than simple solutions to a system in need of dramatic changes. On the other hand, Davidson's analysis of the current state of affairs requires more than mere "plumbing" and addresses the root causes of the crise…
Villiers-sur-Seine
2020
The known Bronze Age settlements between the Île-de-France and Champagne are usually small, unenclosed family farms. The Villiers-sur-Seine site, however, built in a visible and accessible location that enabled control of activities on the nearby river, is completely different: the two large buildings and their annexes were protected by a system of ditches and an ostentatious palisade. Though the Seine Valley was already anthropised at the end of the 9th century BC, this habitat seems to have had a significant impact on the nearby natural environment: development of agropastoral activities due to the extension of wet meadows, episodes of land clearing for construction, cultivation of variou…
Market efficiency and the long-memory of supply and demand: is price impact variable and permanent or fixed and temporary?
2016
In this comment we discuss the problem of reconciling the linear efficiency of price returns with the long-memory of supply and demand. We present new evidence that shows that efficiency is maintained by a liquidity imbalance that co-moves with the imbalance of buyer vs. seller initiated transactions. For example, during a period where there is an excess of buyer initiated transactions, there is also more liquidity for buy orders than sell orders, so that buy orders generate smaller and less frequent price responses than sell orders. At the moment a buy order is placed the transaction sign imbalance tends to dominate, generating a price impact. However, the liquidity imbalance rapidly incre…
Fitting the Stocking Rate with Pastoral Resources to Manage and Preserve Mediterranean Forestlands: A Case Study
2015
Pasture practices have affected Mediterranean forest ecosystems for millennia, and they are still quite widespread in mountainous areas. Nevertheless, in the last decades, the stability of forest ecosystems has been jeopardized due to the abandonment of traditional agro-pastoral practices, so that the gradual reduction of open areas due to progressive succession processes has caused a high increase of grazing pressure by livestock and wild ungulates feeding on forest areas. This paper aims at showing a methodological approach for evaluating the effect of applying measures in order to improve the grazing value of grasslands and ecotonal patches and lower the grazing impact on native woodland…
Environmental mitigation hierarchy and biodiversity offsets revisited through habitat connectivity modelling.
2020
International audience; Biodiversity loss is accelerating because of unceasing human activity and land clearing for development projects (urbanisation, transport infrastructure, mining and quarrying …). Environmental policy-makers and managers in different countries worldwide have proposed the mitigation hierarchy to ensure the goal of “no net loss (NNL) of biodiversity” and have included this principle in environmental impact assessment processes. However, spatial configuration is hardly ever taken into account in the mitigation hierarchy even though it would greatly benefit from recent developments in habitat connectivity modelling incorporating landscape graphs. Meanwhile, national, Euro…
No evidence of systematic pre-emptive loggings after notifying landowners of their lands’ conservation potential
2020
Landowners can intentionally impair biodiversity values occurring on their land to pre-empt biodiversity protection. This often leads to significant negative effects on biodiversity. We studied whether landowners in Finland engaged in pre-emptive loggings after they were notified that their wooded mires are candidate sites for a mire protection program. After the notification, harvesting rates of the candidate wooded mires were significantly lower compared to harvesting rates of similar but non-candidate wooded mires. Annual and monthly harvesting rates indicated that notifying landowners of the conservation potential did not launch systematic pre-emptive logging behavior. Nevertheless, par…
Two simple criteria to estimate an objective's performance when imaging in non design tissue clearing solutions
2019
Tissue clearing techniques are undergoing a renaissance motivated by the need to image fluorescent neurons, and other cells, deep in the sample without physical sectioning. Optical transparency is achieved by equilibrating tissues with high refractive index (RI) solutions. When the microscope objective is not perfectly matched to the RI of the cleared sample, aberrations are introduced. We present two simple-to-calculate numerical criteria predicting: (i) the degradation in image quality (brightness and resolution) from optimal conditions of any clearing solution/objective combination; (ii) which objective, among several available, achieves the highest resolution in a given medium. We deriv…
A Guide to Perform 3D Histology of Biological Tissues with Fluorescence Microscopy
2023
The analysis of histological alterations in all types of tissue is of primary importance in pathology for highly accurate and robust diagnosis. Recent advances in tissue clearing and fluorescence microscopy made the study of the anatomy of biological tissue possible in three dimensions. The combination of these techniques with classical hematoxylin and eosin (H&E) staining has led to the birth of three-dimensional (3D) histology. Here, we present an overview of the state-of-the-art methods, highlighting the optimal combinations of different clearing methods and advanced fluorescence microscopy techniques for the investigation of all types of biological tissues. We employed fluorescence …
Combinatorial Double Auction Radio Resource Allocation Model in Crowd Networks
2018
International audience; Industrial Partners (IPs) with Mobile Network Operators (MNOs) are extending the mobile network infrastructure with Small Cells (SCs) in order to meet the growing mobile traffic demand. Due to the increasing number of telecommunication market competitors and the scarcity of radio resources, static sharing schemes are no more efficient. New dynamic schemes should be considered to meet both user expectations and economic success. In a crowd networking context, we propose in this work a dynamic radio resource scheme based on combinatorial double auctions. The participants in these auctions are the MNOs considered as buyers and the IPs, providers of SCs, considered as se…