Search results for "Coccus"

showing 10 items of 1002 documents

Synthesis and anti-staphylococcal activity of new 4-diazopyrazole derivatives.

2012

Abstract Several new 4-diazopyrazole derivatives 6a – g and 9a – c were obtained by the reaction of 1-(R-substituted-phenyl)-3-(1,3-dimethyl-1 H -pyrazol-5-yl)ureas 5a – g and N -(1,3-dimethyl-1 H -pyrazol-5-yl)-2-(R-substituted-phenyl)acetamides 8a – c respectively with a sevenfold excess of nitrous acid in acetic acid solution. The compounds were assayed for their activity against the Staphylococcus aureus reference strains ATCC 25923, ATCC 29213 and ATCC 6538, as well as six veterinary strains. The best anti-staphylococcal profile was showed by [(R-substituted-phenyl)acetyl](4-diazonio-1,3-dimethyl-1 H -pyrazol-5-yl)azanides 9a , c . Compound 9c was also able at 3.1 μg mL −1 to inhibit o…

PharmacologyNitrous acidStaphylococcus aureusDose-Response Relationship DrugMolecular StructureStereochemistryOrganic ChemistryBiofilmGeneral MedicineMicrobial Sensitivity Testsmedicine.disease_causeSettore BIO/19 - Microbiologia GeneraleSettore CHIM/08 - Chimica FarmaceuticaAnti-Bacterial Agentschemistry.chemical_compoundStructure-Activity RelationshipchemistryStaphylococcus aureusBiofilmsDrug DiscoverymedicinePyrazolesAcetic acid solution4-diazopyrazoles anti-staphylococcal activity anti-biofilm activityAzo CompoundsEuropean journal of medicinal chemistry
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Multidimensional Frailty and Vaccinations in Older People: A Cross-Sectional Study

2022

It is known that influenza, herpes zoster, pneumococcal and pertussis infections may increase morbidity and mortality in older people. Vaccinations against these pathogens are effective in older adults. Frailty seems to be an important determinant of vaccination rates, yet data supporting this association are still missing. Therefore, we aimed to investigate the prevalence of four recommended vaccinations (influenza, herpes zoster, pneumococcal and diphtheria-tetanus-pertussis) and the association with multidimensional frailty assessed using a self-reported comprehensive geriatric assessment tool, i.e., the multidimensional prognostic index (SELFY-MPI). Older participants visiting the outpa…

PharmacologySettore MED/07 - Microbiologia E Microbiologia ClinicaSettore MED/09 - Medicina InternapneumococcuImmunologydiphtheria-tetanus-pertussisherpes zostermultidimensional prognostic index; vaccination; influenza; herpes zoster; pneumococcus; diphtheria-tetanus-pertussisvaccinationSettore MED/42 - Igiene Generale E Applicatamultidimensional prognostic indexdiphtheria-tetanus-pertussivaccination.Infectious DiseasesDrug DiscoveryPharmacology (medical)diphtheria-tetanus-pertussis; herpes zoster; influenza; multidimensional prognostic index; pneumococcus; vaccinationinfluenzapneumococcusVaccines; Volume 10; Issue 4; Pages: 555
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Antimicrobial and antineoplastic activities of new 4-diazopyrazole derivatives

1998

Abstract Several new 4-diazopyrazole derivatives were prepared by the reaction of 3-methyl-5(substituted-benzamido)pyrazoles with an excess of nitrous acid in acetic acid solution. The compounds were tested for antiretroviral activity in HIV-1 infected MT-4 cells and antiproliferative effects against a panel of human leukemia, lymphoma and solid tumor cell lines. They were also tested for activity against representative gram-negative ( Shigella, Salmonella ) and gram-positive ( S. aureus, D group Streptococcus ) bacteria as well as fungi ( C. albicans, C. paratropicalis, C. neoformans and A. fumigatus ). Compounds were devoid of anti HIV-1 and antimicotic activities, whereas they were activ…

PharmacologybiologyChemistryStreptococcusOrganic ChemistryGeneral Medicinemedicine.disease_causebiology.organism_classificationAntimicrobialStreptococcaceaeCorpus albicansMicrobiologyDrug DiscoverymedicineShigellaStaphylococcusBacteriaAntibacterial agentEuropean Journal of Medicinal Chemistry
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One-step synthesis, crystallographic studies and antimicrobial activity of new 4-diazopyrazole derivatives

1996

Summary A number of new 4-diazopyrazole derivatives were prepared by the reaction of 1- R -3-methyl-5(R 1 -substituted)benzamidopyrazoles with a sevenfold excess of nitrous acid in acetic medium. The compounds were tested for activity against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus faecalis, Listeria monocytogenes, Candida albicans, Candida tropicalis and Paecilomyces varioti . The highest microbial susceptibility was shown by Gram-positive bacteria, with minimum inhibitory concentrations (MIC) in the range 0.5–12.5 μg/mL. For S aureus the R 1 substituents were screened utilizing the Topliss operational scheme. The 4-nitro g…

PharmacologybiologyStereochemistryChemistryOrganic ChemistryGeneral Medicinebiology.organism_classificationAntimicrobialmedicine.disease_causeCandida tropicalisStaphylococcus epidermidisStaphylococcus aureusDrug DiscoverymedicineCandida albicansAntibacterial activityEscherichia coliAntibacterial agentEuropean Journal of Medicinal Chemistry
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Polyphasic taxonomy of a novel yeast isolated from antarctic environment; description of Cryptococcus victoriae sp. nov.

1999

In 1992 some samples of mosses, lichens and soils were collected from Botany Bay, Southern Victoria Land (77 degrees 01' S 162 degrees 32' E) and, as a result of a routine screening programme some yeasts were isolated. One of them, designated as strain G5, showed marked differences when compared to other antarctic yeasts. According to morphological and physiological characteristics, we were able to identify the strain G5 as a yeast belonging to the genus Cryptococcus. Some characteristics of this genus are the growth response to myo-inositol, celobiose, raffinose and D-glucuronate, no-fermentation, the absence of mycelium and pseudomycelium, asexual reproduction, Diazolium blue B test (DBB)…

Phylogenetic treeBase SequenceMolecular Sequence DataFungi imperfectiRibosomal RNABiologybiology.organism_classificationApplied Microbiology and BiotechnologyMicrobiologyPolymerase Chain ReactionYeastCryptococcusPhenotypePhylogeneticsBotanyTaxonomy (biology)LichenDNA FungalEcology Evolution Behavior and SystematicsMyceliumPolymorphism Restriction Fragment LengthSystematic and applied microbiology
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Complete Genome Sequence of Acidaminococcus intestini RYC-MR95, a Gram-Negative Bacterium from the Phylum Firmicutes

2011

ABSTRACT Acidaminococcus intestini belongs to the family Acidaminococcaceae , order Selenomonadales , class Negativicutes , phylum Firmicutes . Negativicutes show the double-membrane system of Gram-negative bacteria, although their chromosomal backbone is closely related to that of Gram-positive bacteria of the phylum Firmicutes . The complete genome of a clinical A. intestini strain is here presented.

Phylum FirmicutesMolecular Sequence DataVeillonellaceaeBiologyMicrobiologyGenomeMicrobiologyEvolution Molecular03 medical and health sciencesGram negative bacteriumHumansAcidaminococcusMolecular Biology030304 developmental biologyGeneticsWhole genome sequencing0303 health sciencesAcidaminococcus intestiniNegativicutesBase Sequence030306 microbiologybiology.organism_classificationGenome AnnouncementsGram-Negative Bacterial InfectionsGenome BacterialBacteriaJournal of Bacteriology
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Nicotinamide Adenine Dinucleotide-Dependent and Nicotinamide Adenine Dinucleotide-Independent Lactate Dehydrogenases in Homofermentative and Heterofe…

1971

Three homofermentative ( Lactobacillus plantarum B38, L. plantarum B33, Pediococcus pentosaceus B30) and three heterofermentative ( Leuconostoc mesenteroides 39, L. oenos B70, Lactobacillus brevis ) lactic acid bacteria were examined for the presence or absence of nicotinamide adenine dinucleotide (NAD)-dependent and NAD-independent d - and l -lactate dehydrogenases. Two of the six strains investigated, P. pentosaceus and L. oenos , did not exhibit an NAD-independent enzyme activity capable of reducing dichlorophenol indophenol. The p H optima of the lactic dehydrogenases were determined. The NAD-dependent enzymes from homofermentative strains exhibited optima at p H 7.8 to 8.8, whereas va…

Physiology and MetabolismNicotinamide adenine dinucleotideMicrobiologychemistry.chemical_compoundSpecies SpecificityLactobacillusChemical PrecipitationLeuconostocPediococcusProtaminesMolecular BiologyCell-Free SystemL-Lactate DehydrogenasebiologySulfatesLactobacillus brevisfood and beveragesStereoisomerismHydrogen-Ion ConcentrationNADbiology.organism_classificationCulture MediaLactic acidLactobacillusIndophenolBiochemistrychemistryAmmonium SulfateSpectrophotometryFermentationLactatesPediococcusNAD+ kinaseLeuconostocLactobacillus plantarumJournal of Bacteriology
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PCR for the detection of pathogens in neonatal early onset sepsis.

2020

Background A large proportion of neonates are treated for presumed bacterial sepsis with broad spectrum antibiotics even though their blood cultures subsequently show no growth. This study aimed to investigate PCR-based methods to identify pathogens not detected by conventional culture. Methods Whole blood samples of 208 neonates with suspected early onset sepsis were tested using a panel of multiplexed bacterial PCRs targeting Streptococcus pneumoniae, Streptococcus agalactiae (GBS), Staphylococcus aureus, Streptococcus pyogenes (GAS), Enterobacteriaceae, Enterococcus faecalis, Enterococcus faecium, Ureaplasma parvum, Ureaplasma urealyticum, Mycoplasma hominis and Mycoplasma genitalium, a …

PhysiologyArtificial Gene Amplification and ExtensionPathology and Laboratory Medicinemedicine.disease_causePolymerase Chain ReactionUreaplasmaUreaplasmaMycoplasma0302 clinical medicineAntibioticsRNA Ribosomal 16SMedicine and Health Sciences030212 general & internal medicineAge of OnsetCandidaMultidisciplinaryNeonatal sepsisAntimicrobialsQCandidiasisRDrugsPneumococcusBacterial InfectionsBacterial PathogensBody FluidsBloodMedical MicrobiologyInfant Extremely PrematureMedicinePathogensNeonatal SepsisAnatomyInfant PrematureResearch ArticleStaphylococcus aureusScienceMycoplasma hominisBiologyResearch and Analysis MethodsReal-Time Polymerase Chain ReactionMicrobiologyDNA RibosomalSensitivity and SpecificityMicrobiology03 medical and health sciencesSigns and SymptomsEnterobacteriaceaeDiagnostic MedicineSepsisMicrobial Control030225 pediatricsStreptococcus pneumoniaemedicineHumansMolecular Biology TechniquesMicrobial PathogensMolecular BiologyPharmacologyBacteriaOrganismsInfant NewbornBiology and Life SciencesNeonatesStreptococcusMycoplasmamedicine.diseasebiology.organism_classificationEarly DiagnosisStreptococcus agalactiaeMultiplex Polymerase Chain ReactionEnterococcusDevelopmental BiologyUreaplasma urealyticumEnterococcus faecium
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Bifunctional dentifrice: Amorphous polyphosphate a regeneratively active sealant with potent anti- Streptococcus mutans activity

2017

Abstract Objective In this study we demonstrate that inorganic polyphosphate (polyP) exhibits a dual protective effect on teeth: it elicits a strong antibacterial effect against the cariogenic bacterium Streptococcus mutans and, in form of amorphous calcium polyP microparticles (size of 100–400 nm), it efficiently reseals cracks/fissures in the tooth enamel and dentin. Methods Three different formulations of amorphous polyP microparticles (Ca-polyP, Zn-polyP and Sr-polyP) were prepared. Results Among the different polyP microparticles tested, the Ca-polyP microparticles, as a component of a newly developed formulation of a dentifrice, turned out to be most effective in inhibiting growth of …

Pit and Fissure SealantsMaterials sciencebusiness.product_category02 engineering and technologyDental CariesMicrobiologyStreptococcus mutans03 medical and health scienceschemistry.chemical_compound0302 clinical medicinePolyphosphatesotorhinolaryngologic diseasesDentinmedicineDentifriceGeneral Materials ScienceDental EnamelneoplasmsGeneral DentistryDentifricesToothpastebiologyPolyphosphateBiofilmpathological conditions signs and symptoms030206 dentistry021001 nanoscience & nanotechnologybiology.organism_classificationTooth enamelStreptococcus mutansdigestive system diseasessurgical procedures operativemedicine.anatomical_structurechemistryMechanics of MaterialsBiofilms0210 nano-technologybusinessAntibacterial activityNuclear chemistryDental Materials
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Identification of a plasminogen-binding motif in PAM, a bacterial surface protein.

1995

Surface-associated plasmin(ogen) may contribute to the invasive properties of various cells. Analysis of plasmin(ogen)-binding surface proteins is therefore of interest. The N-terminal variable regions of M-like (ML) proteins from five different group A streptococcal serotypes (33, 41, 52, 53 and 56) exhibiting the plasminogen-binding phenotype were cloned and expressed in Escherichia coli. The recombinant proteins all bound plasminogen with high affinity. The binding involved the kringle domains of plasminogen and was blocked by a lysine analogue, 6-aminohexanoic acid, indicating that lysine residues in the M-like proteins participate in the interaction. Sequence analysis revealed that the…

PlasminStreptococcus pyogenesMolecular Sequence DataPlasma protein bindingBiologyMicrobiologyKringle domainBacterial ProteinsKringlesmedicineEscherichia coliAmino Acid SequenceBinding siteCloning MolecularMolecular BiologyPeptide sequenceBinding SitesBase SequenceLysinePlasminogenFusion proteinMolecular biologyRecombinant ProteinsPhenotypeBiochemistryCarrier ProteinsPlasminogen activatorSequence AlignmentBinding domainmedicine.drugProtein BindingMolecular microbiology
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