Search results for "Coding"

DiseaseLinc: Disease Enrichment Analysis of Sets of Differentially Expressed LincRNAs

2021

Long intergenic non-coding RNAs (LincRNAs) are long RNAs that do not encode proteins. Functional evidence is lacking for most of them. Their biogenesis is not well-known, but it is thought that many lincRNAs originate from genomic duplication of coding material, resulting in pseudogenes, gene copies that lose their original function and can accumulate mutations. While most pseudogenes eventually stop producing a transcript and become erased by mutations, many of these pseudogene-based lincRNAs keep similarity to the parental gene from which they originated, possibly for functional reasons. For example, they can act as decoys for miRNAs targeting the parental gene. Enrichment analysis of fun…

Compact and Field Portable Biophotonic Sensors for Automated Cell Identification (Plenary Address)

2021

In this Plenary address paper, we overview recently published work for automated cell identification using 3D optical imaging in compact and field portable biophotonic sensors. Digital holographic microscopy systems and lensless pseudorandom phase encoding systems capture 3D information of biological cells and make highly accurate automated cell identification possible. Overviewed systems include sickle cell disease diagnosis based on spatio-temporal cell dynamics in a field-portable 3D-printed shearing digital holography as well as lensless cell identification of both single and multicell samples using pseudorandom phase encoding.

Understanding unethical behaviors at the university level: a multiple regression analysis

2020

Unethical behaviors such as corruption pose an important challenge for students, professors, and other university members. We aimed to clarify students' willingness to engage in corruption in a Spanish public university. In all, 3,475 undergraduate, postgraduate, and PhD students completed an online questionnaire assessing four corruption scenarios: favoritism, bribery, fraud, and embezzlement. Multiple regression analysis suggested that justifiability, risk perception, and perceived corruption played a key role in explaining corrupt intention. Behavioral intention to engage in corruption is a complex phenomenon explained by not only peers' behaviors, but also individuals' justifications of…

Online Consultations Between General Practitioners and Psychiatrists in the Netherlands

2021

Objective: To examine the nature and scope of questions about psychiatric patient cases submitted by general practitioners (GPs) to an established online consultation platform and to determine if they could have been answered by consulting existing clinical guidelines.Methods: All anonymized psychiatric cases submitted by GPs to the online electronic Prisma platform between September 2018 and November 2019 were examined in a mixed-methods study. Descriptive statistics and qualitative thematic analysis were used, followed by axial coding to arrive at overarching themes to characterize cases.Results: Of the 136 included cases, 44.1% concerned female patients and about half concerned patients …

Do Transposed-Letter Similarity Effects Occur at a Syllable Level?

2006

One key issue for any computational model of visual word recognition is the choice of an input coding scheme for assigning letter position. Recent research has shown that transposed-letter similarity effects occur even when the transposed letters are not adjacent (caniso- casino; Perea & Lupker, 2004 , JML). In the present study we conducted two single-presentation lexical decision experiments to examine whether transposed-letter effects occur at a syllable level. We tested two types of nonwords: (1) nonwords created by transposing two internal CV syllables (PRIVEMARA; the base word is primavera, the Spanish for spring) and (2) nonwords created by transposing two adjacent bigrams that …

The Innovative Moments Coding System and the Assimilation of Problematic Experiences Scale: A case study comparing two methods to track change in psy…

2014

The Assimilation of Problematic Experiences Scale (APES) and the Innovative Moments Coding System were applied to transcripts of a successful case of linguistic therapy of evaluation independently by different research groups. Assimilation theory and research suggest that higher APES scores reflect therapeutic gains, with a level of approximately 4.0 separating good from poor outcome cases. The innovative moments (IMs) model suggests that IMs classified as reconceptualization and performing change occur mainly in good outcome cases, whereas action, reflection and protest occur in both good and poor outcome cases. Passages coded as reconceptualization and performing change were rare in this …

Long non-coding RNA and messenger RNA—the meeting of two worlds

2018

Long non-coding RNAs (lncRNAs), defined as transcripts without protein-coding potential, have recently emerged as key mediators in numerous biological functions. LncRNAs act as regulators of gene expression at different levels including transcriptional and post-transcriptional regulation as well as chromatin organization.

Molecular analysis of genes encoding CFTR interactors of SLC26 family in CF patients: preliminary results

2008

Reconnoitering the Role of Long-Noncoding RNAs in Hypertrophic Cardiomyopathy: A Descriptive Review

2021

Hypertrophic cardiomyopathy (HCM) is the most common form of hereditary cardiomyopathy. It is characterized by an unexplained non-dilated hypertrophy of the left ventricle with a conserved or elevated ejection fraction. It is a genetically heterogeneous disease largely caused by variants of genes encoding for cardiac sarcomere proteins, including MYH7, MYBPC3, ACTC1, TPM1, MYL2, MYL3, TNNI3, and TNNT23. Preclinical evidence indicates that the enhanced calcium sensitivity of the myofilaments plays a key role in the pathophysiology of HCM. Notably, this is not always a direct consequence of sarcomeric variations but may also result from secondary mutation-driven alterations. Long non-coding R…

Zebrafish as a Model to Evaluate a CRISPR/Cas9-Based Exon Excision Approach as a Future Treatment Option for EYS-Associated Retinitis Pigmentosa

2021

Retinitis pigmentosa (RP) is an inherited retinal disease (IRD) with an overall prevalence of 1 in 4000 individuals. Mutations in EYS (Eyes shut homolog) are among the most frequent causes of non-syndromic autosomal recessively inherited RP and act via a loss-of-function mechanism. In light of the recent successes for other IRDs, we investigated the therapeutic potential of exon skipping for EYS-associated RP. CRISPR/Cas9 was employed to generate zebrafish from which the region encompassing the orthologous exons 37-41 of human EYS (eys exons 40-44) was excised from the genome. The excision of these exons was predicted to maintain the open reading frame and to result in the removal of exactl…