Search results for "Colcemid"
showing 4 items of 4 documents
Functional Inactivation of pRB Results in Aneuploid Mammalian Cells After Release From a Mitotic Block
2002
AbstractThe widespread chromosome instability observed in tumors and in early stage carcinomas suggests that aneuploidy could be a prerequisite for cellular transformation and tumor initiation. Defects in tumor suppressers and genes that are part of mitotic checkpoints are likely candidates for the aneuploid phenotype. By using flow cytometric, cytogenetic, immunocytochemistry techniques we investigated whether pRB deficiency could drive perpetual aneuploidy in normal human and mouse fibroblasts after mitotic checkpoint challenge by microtubule-destabilizing drugs. Both mouse and human pRB-deficient primary fibroblasts resulted, upon release from a mitotic block, in proliferating aneuploid …
Monitoring of the action of drugs in melanoma cells by dynamic laser speckle
2014
Abstract. This work presents the development of a protocol based on the dynamic laser speckle designed tomonitor the reaction of cancer cells of line MEL-RC08 to the application of the drug Colcemid in two differentconcentrations: 0.2 and 0.4 μg∕mL. The protocol was designed using the forward scattering approach with anHe-Ne laser of 632.8 nm illuminating the samples, a control, and two variations of Colcemid, being monitoredalong 8 h. The data were analyzed numerically in the time and in the frequency domain, and the results pre-sented the ability of the technique to monitor the action of the drug, particularly Colcemid (0.4 μg∕mL). © 2014Society of Photo-Optical Instrumentation Engineers …
Microtubules and intermediate filaments of herpes simplex virus infected cells.
1987
The fate of microtubules and of vimentin or keratin containing intermediate filaments during infection with fusion or rounding producing strains of herpes simplex virus (HSV) was investigated. Microtubules polymerize early after fusion of cells. However, they do not reconstitute 6–7 hours post infection (p.i.) after release of a colcemid block. Keratin and vimentin are maintained around the original nucleus still inside of recruited cells in the polykaryocyte. Cells of fibroblastic and epithelial origin fuse. Inside of polykaryocytes keratin or vimentin containing fibers seem to polymerize. Keratin is to be found in invaginations in the nuclei surrounded by the inner layer of the nuclear me…
Dis-organizing centrosomal clusters: specific cancer therapy for a generic spread?
2015
Cancer is a leading cause of mortality and the annual incidence of new cancer cases is rising worldwide. Due to the frequent development of resistance and the side effects of established anti-cancer drugs, the quest for new drugs with improved therapeutic features goes on. In contrast to cytotoxic chemotherapy of the past, the concept of targeted chemotherapy attempts to increase specificity of therapy by attacking tumor-related mechanisms. A novel emerging treatment concept represents the inhibition of centrosomal clustering. The centrosome regulates mitotic spindle formation assuring uniform separation of chromosomes to daughter cells. Many tumors contain supernumerary centrosomes, which …