6533b837fe1ef96bd12a2778

RESEARCH PRODUCT

Monitoring of the action of drugs in melanoma cells by dynamic laser speckle

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Abstract. This work presents the development of a protocol based on the dynamic laser speckle designed tomonitor the reaction of cancer cells of line MEL-RC08 to the application of the drug Colcemid in two differentconcentrations: 0.2 and 0.4 μg∕mL. The protocol was designed using the forward scattering approach with anHe-Ne laser of 632.8 nm illuminating the samples, a control, and two variations of Colcemid, being monitoredalong 8 h. The data were analyzed numerically in the time and in the frequency domain, and the results pre-sented the ability of the technique to monitor the action of the drug, particularly Colcemid (0.4 μg∕mL). © 2014Society of Photo-Optical Instrumentation Engineers (SPIE) [DOI: 10.1117/1.JBO.19.5.057008] Keywords: specke patterns; dynamic laser speckle; cellular activity; Colcemid.Paper 140040R received Jan. 24, 2014; revised manuscript received Apr. 3, 2014; accepted for publication Apr. 23, 2014; publishedonline May 14, 2014. 1 Introduction The preclinical models of the action of drugs in medicine isa key point to evaluate the effectiveness of those drugs overtissues or cells, such as tumors, before their adoption in serialtreatments. The advanced evaluation allows a thorough knowl-edgeofthecells’responseandinadditionallowstheadjustmentof the optimum concentration of the chemicals. In order toachieve the models, tests can be carried out in cells in vivomerged in a nutritive medium, which creates an adequate con-dition for the cells to survive and to introduce different drugs.The monitoring of the drugs and of the cell interactions can bedonebymeansofsomeroutinetechniques,suchaschemotaxis,