Search results for "Complement"
showing 10 items of 2113 documents
Molecular determinants of the Arabidopsis AKT1 K+ channel ionic selectivity investigated by expression in yeast of randomly mutated channels
1999
International audience; The Avabidopsis thaliana K+ channel AKT1 was expressed in a yeast strain defective for K+ uptake at low K+ concentrations (<3 mM). Besides restoring K+ transport in this strain, AKT1 expression increased its tolerance to salt (NaCl or LiCl), whatever the external K+ concentration used (50 mu M, 5 mM, or 50 mM), We took advantage of the latter phenomenon for screening a library of channels randomly mutated in the region that shares homologies with the pore forming domain (the so-called P domain) of animal K+ channels (Shaker family). Cassette mutagenesis was performed using a degenerate oligonucleotide that was designed to ensure, theoretically, a single mutation per …
Current view of nitric oxide-responsive genes in plants
2009
International audience; Significant efforts have been directed towards the identification of genes differentially regulated through nitric oxide (NO)-dependent processes. These efforts comprise the use of medium- and large-scale transcriptomic analyses including microarray and cDNA-amplification fragment length polymorphism (AFLP) approaches. Numerous putative NO-responsive genes have been identified in plant tissues and cell suspensions with transcript levels altered by artificially released NO, or endogenously produced. Comparative analysis of the data from such transcriptomic analyses in Arabidopsis reveals that a significant part of these genes encode proteins related to plant adaptive …
Construction and validation of cDNA-based Mt6k-RIT macro- and microarrays to explore root endosymbioses in the model legume Medicago truncatula
2004
To construct macro- and microarray tools suitable for expression profiling in root endosymbioses of the model legume Medicago truncatula, we PCR-amplified a total of 6048 cDNA probes representing genes expressed in uninfected roots, mycorrhizal roots and young root nodules [Nucleic Acids Res. 30 (2002) 5579]. Including additional probes for either tissue-specific or constitutively expressed control genes, 5651 successfully amplified gene-specific probes were used to grid macro- and to spot microarrays designated Mt6k-RIT (M. truncatula 6k root interaction transcriptome). Subsequent to a technical validation of microarray printing, we performed two pilot expression profiling experiments usin…
Regulation of reactive oxygen species production by a 14-3-3 protein in elicited tobacco cells.
2007
International audience; The regulation of the system responsible for the production of reactive oxygen species (ROS) during plant–microorganism interaction is still largely unknown. The protein NtrbohD has been recently demonstrated as the plasma membrane oxidase responsible for ROS production in elicited tobacco cells. Here, its C-terminus part was used as a bait in a two-hybrid screen in order to identify putative regulators of this system. This led to the isolation of a cDNA coding for a member of the 14-3-3 protein family. The corresponding transcript was induced after infiltration of tobacco leaves with the fungal elicitor cryptogein. Tobacco cells transformed with an antisense constru…
The plasma membrane oxidase NtrbohD is responsible for AOS production in elicited tobacco cells
2002
Summary A cDNA encoding a protein, NtrbohD, located on the plasma membrane and homologue to the flavocytochrome of the neutrophil NADPH oxidase, was cloned in tobacco. The corresponding mRNA was accumulated when tobacco leaves and cells were treated with the fungal elicitor cryptogein. After elicitation with cryptogein, tobacco cells transformed with antisense constructs of NtrbohD showed the same extracellular alkalinization as the control, but no longer produced active oxygen species (AOS). This work represents the first demonstration of the function of a homologue of gp91–phox in AOS production in elicited tobacco cells.
Large birds travel farther in homogeneous environments
2019
Aim: Animal movement is an important determinant of individual survival, population dynamics and ecosystem structure and function. Nonetheless, it is still unclear how local movements are related to resource availability and the spatial arrangement of resources. Using resident bird species and migratory bird species outside the migratory period, we examined how the distribution of resources affects the movement patterns of both large terrestrial birds (e.g., raptors, bustards and hornbills) and waterbirds (e.g., cranes, storks, ducks, geese and flamingos). Location: Global. Time period: 2003–2015. Major taxa studied: Birds. Methods: We compiled GPS tracking data for 386 individuals across 3…
Rodzinna postpamięć Ślązaków
2017
Family Postmemory of the People of Silesia
 The author suggests a possible use of postmemory to analyse the contemporary family memory of the people of Silesia. Their memoirs about WW2 (such as working for the Wehrmacht, the Red Army invading Silesia, working in labour camps, nationality verification, displacements to Germany, and deportations to Siberia) bear signs of latent memory which is rarely revealed even to the next of kin. Present mainly within the family circle, within the local society, and among friends, these memoirs integrated Silesians and made them a unique community that considers itself a stigmatized minority. This contributed to mythologizing and stereotyping the who…
The Role the Static Maximization Approach Plays in Neoclassical Analyses
1994
This is an article on the methodology of economic thought. The critical assessment of the neoclassical research programme contained here basically comes from the contributions of J.M. Buchanan, Nobel prize winner in Economics 1986. These comments are aimed at pointing out the role that the static maximization approach plays in neoclassical analyses since L. Robbins and P. Samuelson’s influential contributions came about after World War II. Just to complement this basic purpose, I present in section 4 the alternative methodological foundations J.M. Buchanan proposes and uses to replace the static maximization approach when building public choice analyses and I sketch in section 5 several pe…
From “Mano Dura” to “Enfrentamiento Directo”: swings of public policies in El Salvador
2019
RESUMEN Introduccion: El presente trabajo analiza las cuatro principales politicas publicas de seguridad contra las pandillas en El Salvador: Mano Dura (2003-2004), Super Mano Dura (2004-2009), Tregua entre pandillas (2012-2014) y Enfrentamiento Directo (2014-2019). El objetivo es identificar la influencia de estas politicas sobre la violencia. Materiales y Metodos: Se trata de una investigacion cualitativa apoyada en 12 entrevistas y el relato de vida con las que se explora la responsabilidad gubernamental frente al problema de la violencia en El Salvador. El trabajo de campo se efectuo durante 7 meses, entre septiembre de 2016 y marzo de 2017. El tratamiento de la informacion se efectuo m…
Morfofisiología del diafragma torácico en las medicinas china y occidental
2016
Resumen Las autoras creen que el analisis comparativo de diversos temas entre la medicina china y la occidental supone una aportacion al progresivo —y deseado— avance hacia la mutua comprension y sinergia entre ambas disciplinas. La descripcion occidental del diafragma toracico sugiere ciertas lineas de confluencia respecto al enfoque chino: anatomicamente aportan sugerentes paralelismos las referencias de insercion muscular e inervacion del diafragma, asi como su biodinamica respiratoria, elementos coincidentes con la posicion de diversos puntos de acupuntura toracicos que presentan, ademas, posibles semejanzas en sus funciones fisiologicas reguladoras a diversos niveles, con las que la me…