Search results for "Complement"
showing 10 items of 2113 documents
Alpha- and gamma-interferon (IFNα, IFNγ) but not interleukin-1 (IL-1) modulate synthesis and secretion of β2-microglobulin by hepatocytes
1988
Soluble serum beta 2-microglobulin has been thought to result from membrane shedding by activated T-lymphocytes. This hypothesis could explain the increase of beta 2-microglobulin serum levels during virally induced mononucleosis, but not elevated levels as observed in other virally induced and in malignant diseases. In this paper we demonstrate that beta 2-microglobulin is a true secretory protein, and that its synthesis in hepatocytes is modulated by IFNs but not by IL-1. While the 45,000 MW HLA antigen can be found only in cell lysates, beta 2-microglobulin is shown to be secreted also into the culture medium like other secretory proteins (e.g. albumin-factor B-complement C3). Furthermor…
Cloning and sequencing of the chicken egg-white avidin-encoding gene and its relationship with the avidin-related genes Avrl-Avr5
1995
Abstract The gene encoding chicken egg-white avidin (Avd) was amplified from chromosomal DNA, cloned and sequenced. The entire coding region of preavidin (pre-Avd) containing four exons was identified by comparing the Avd gene (1119 bp) with the cDNA. It had a high identity percentage (91–95%) with the previously isolated Avd-related genes 1–5 (Avrl–Avr5) . Interestingly, comparison of Avd with the Avr genes showed that the introns were better conserved (on average 97%) than the exons (90%). The Avd gene, as well as the cDNA, encodes a Gln residue at position 53 of the mature protein, which is in contrast to the previously determined amino-acid sequence.
A Novel Chitin-binding Protein from the Vestimentiferan Riftia pachyptila Interacts Specifically with β-Chitin
2001
Abstract A cDNA from Riftia pachyptila was cloned. It encodes a novel 21.3-kDa protein from the worm protective tube, named RCBP (for Riftia chitin-binding protein). On the basis of partial tube-peptide sequences previously obtained, experiments using reverse transcriptase-mediated polymerase chain reaction and rapid amplification of cDNA ends led to the complete cDNA sequence. Analysis of its deduced amino acid sequence shows the presence of two chitin-binding domains. These domains are closely related to type 2 chitin-binding domains that are restricted to the animal kingdom. We showed by affinity assay and immunogold labeling that RCBP is the first protein so far known that binds specifi…
cDNA Synthesis and Cloning
1998
The isolation of intact messenger RNA and its conversion into cDNA copies by avian or Moloney murine reverse transcriptase, as well as subsequent amplification of gene transcripts by the PCR technique, are becoming increasingly important tools in molecular biology. At present, these techniques have been often necessary and widely used for the analysis of individual mRNA levels in cells and tissues by Northern blot analysis, nuclease protection analysis and in situ hybridization. Another important application of RNA templates is the construction of representative cDNA libraries in order to clone genes, to investigate their molecular structure and to express them in prokaryotic and/or eukary…
Receptor Tyrosine Kinase, an Autapomorphic Character of Metazoa: Identification in Marine Sponges
1999
In the present review we summarize sequence data obtained from cloning of sponge receptor tyrosine kinases [RTK]. The cDNA sequences were mainly obtained from the marine sponge Geodia cydonium. RTKs (i) with immunoglobulin [Ig]-like domains in the extracellular region, (ii) of the type of insulinlike receptors, as well as (iii) RTKs with one extracellular speract domain, have been identified. The analyses revealed that the RTK genes are constructed in blocks [domains], suggesting a blockwise evolution. The phylogenetic relationships of the sequences obtained revealed that all sponge sequences fall into one branch of the evolutionary tree, while related sequences from higher Metazoa, human, …
Semmes surfaces and intrinsic Lipschitz graphs in the Heisenberg group
2018
A Semmes surface in the Heisenberg group is a closed set $S$ that is upper Ahlfors-regular with codimension one and satisfies the following condition, referred to as Condition B. Every ball $B(x,r)$ with $x \in S$ and $0 < r < \operatorname{diam} S$ contains two balls with radii comparable to $r$ which are contained in different connected components of the complement of $S$. Analogous sets in Euclidean spaces were introduced by Semmes in the late $80$'s. We prove that Semmes surfaces in the Heisenberg group are lower Ahlfors-regular with codimension one and have big pieces of intrinsic Lipschitz graphs. In particular, our result applies to the boundary of chord-arc domains and of redu…
GenClust: A genetic algorithm for clustering gene expression data
2005
Abstract Background Clustering is a key step in the analysis of gene expression data, and in fact, many classical clustering algorithms are used, or more innovative ones have been designed and validated for the task. Despite the widespread use of artificial intelligence techniques in bioinformatics and, more generally, data analysis, there are very few clustering algorithms based on the genetic paradigm, yet that paradigm has great potential in finding good heuristic solutions to a difficult optimization problem such as clustering. Results GenClust is a new genetic algorithm for clustering gene expression data. It has two key features: (a) a novel coding of the search space that is simple, …
A musical reading of a contemporary installation and back: mathematical investigations of patterns in Qwalala
2021
Mathematical music theory helps us investigate musical compositions in mathematical terms. Some hints can be extended towards the visual arts. Mathematical approaches can also help formalize a "translation" from the visual domain to the auditory one and vice versa. Thus, a visual artwork can be mathematically investigated, then translated into music. The final, refined musical rendition can be compared to the initial visual idea. Can an artistic idea be preserved through these changes of media? Can a non-trivial pattern be envisaged in an artwork, and then still be identified after the change of medium? Here, we consider a contemporary installation and an ensemble musical piece derived from…
The Jordan-Hölder theorem and prefrattini subgroups of finite groups
1995
by A. BALLESTER-BOLINCHES and L. M. EZQUERRO(Received 26 January, 1994)Introduction. All groups considered are finite. In recent years a number ofgeneralizations of the classic Jordan-Holder Theorem have been obtained (see [7],Theorem A.9.13): in a finite group G a one-to-one correspondence as in the Jordan-Holder Theorem can be defined preserving not only G-isomorphic chief factors but eventheir property of being Frattini or non-Frattini chief factors. In [2] and [13] a newdirection of generalization is presented: the above correspondence can be defined in sucha way that the corresponding non-Frattini chief factors have the same complement(supplement).In this paper we present a necessary a…
Incomplete vertices in the prime graph on conjugacy class sizes of finite groups
2013
Abstract Given a finite group G, consider the prime graph built on the set of conjugacy class sizes of G. Denoting by π 0 the set of vertices of this graph that are not adjacent to at least one other vertex, we show that the Hall π 0 -subgroups of G (which do exist) are metabelian.