Search results for "Complex."
showing 10 items of 5824 documents
Simultaneous stimulation of GABA and beta adrenergic receptors stabilizes isotypes of activated adenylyl cyclase heterocomplex
2004
Abstract Background We investigated how the synthesis of cAMP, stimulated by isoproterenol acting through β-adrenoreceptors and Gs, is strongly amplified by simultaneous incubation with baclofen. Baclofen is an agonist of δ-aminobutyric acid type B receptors [GABAB], known to inhibit adenylyl cyclase via Gi. Because these agents have opposite effects on cAMP levels, the unexpected increase in cAMP synthesis when they are applied simultaneously has been intensively investigated. From previous reports, it appears that cyclase type II contributes most significantly to this phenomenon. Results We found that simultaneous application of isoproterenol and baclofen specifically influences the assoc…
Reference set of Mycobacterium tuberculosis clinical strains: A tool for research and product development
2018
TheMycobacterium tuberculosiscomplex (MTBC) causes tuberculosis (TB) in humans and various other mammals. The human-adapted members of the MTBC comprise seven phylogenetic lineages that differ in their geographical distribution. There is growing evidence that this phylogenetic diversity modulates the outcome of TB infection and disease. For decades, TB research and development has focused on the two canonical MTBC reference strains H37Rv and Erdman, both of which belong to Lineage 4. Relying on only a few laboratory-adapted strains can be misleading as study results might not be directly transferrable to clinical settings where patients are infected with a diverse array of strains, includin…
Small GTP-binding proteins of the Rho- and Ras-subfamilies are not involved in the actin rearrangements induced by attaching and effacingEscherichia …
1998
Attaching and effacing Escherichia coli (AEEC) are extracellular pathogens that induce the formation of actin-rich structures at their sites of attachment to eukaryotic host cells. We analysed whether small GTP-binding proteins of the Rho- and Ras-subfamilies, which control the cellular actin system, are essential for these bacterial-induced microfilament reorganizations. For this purpose we specifically inactivated them using the Clostridium difficile toxins TcdB-10463 and TcdB-1470. Such treatment led to a dramatic breakdown of the normal actin cytoskeleton, but did not abrogate the bacterial-induced actin rearrangements. Our data therefore indicate that the microfilament reorganizations …
Interplay of omnivory, energy channels and C availability in a microbial-based soil food web
1998
To study the effects of omnivory on the structure and function of soil food webs and on the control of trophic-level biomasses in soil, two food webs were established in microcosms. The first one contained fungi, bacteria, a fungivorous nematode (Aphelenchoides saprophilus) and a bacterivorous nematode (Caenorhabditis elegans), and the second one fungi, bacteria, the fungivore and an omnivorous nematode (Mesodiplogaster sp.) feeding on both bacteria and the fungivore. Half of the replicates of each food web received additional glucose. The microcosms were sampled destructively at 5, 9, 13 and 19 weeks to estimate the biomass of microbes and nematodes and the soil NH4+-N concentration. The e…
Effects of microbivore species composition and basal resource enrichment on trophic-level biomasses in an experimental microbial-based soil food web.
1998
Previous theoretical and empirical evidence suggests that species composition within trophic levels may profoundly affect the response of trophic-level biomasses to enhanced basal resources. To test whether species composition of microbivorous nematodes has such an effect in microbial-based soil food webs, I created three microcosm food webs, consisting of bacteria, fungi, bacterial-feeding nematodes (Acrobeloides tricornus, Caenorhabditis elegans), fungal-feeding nematodes (Aphelenchus avenae, Aphelenchoides sp.) and a predatory nematode (Prionchulus punctatus). The food webs differed in species composition at the second trophic level: food web A included A. tricornus and Aph. avenae, food…
The Messinian stromatolites of the Sierra del Colmenar (Western Mediterranean): facies characterization and sedimentological interpretation
2018
A representative outcrop of the Messinian stromatolites belonging to the Terminal Carbonate Complex unit, from the northern sector of the Bajo Segura basin (Caja de Ahorros del Mediterraneo section, Sierra del Colmenar, SE Spain) has been studied. Here, we present a detailed analysis of the architecture, external morphology, and internal morphology in order to reconstruct the environmental and palaeoecological conditions for their growth. The stromatolites macrostructure consists of a continuously doming type morphology (build up and sheets areas).These developed close to the coast and acted as a palaeogeographic barrier, reducing physical stress, channeling the erosive effect of water and …
Effects of forest restoration treatments on the abundance of bark beetles in Norway spruce forests of southern Finland
2009
Abstract Restoration of protected areas in boreal forests frequently includes creating substantial volumes of dead wood. While this benefits a wide range of dead wood dependent invertebrate species, some of these are regarded as forest pests. Therefore, the risk of elevated levels of tree mortality in surrounding commercial forests must be considered. In a large-scale field experiment in southern Finland, we studied the effects of restoration treatments on the abundance of bark beetles within and in the vicinity of restored areas, in particular focusing on Ips typographus and Pityogenes chalcographus . The treatments applied to managed Norway spruce forests were controlled burning and parti…
Geochemistry, petrogenesis and tectonic setting of the Samothraki mafic suite, NE Greece: Trace-element, isotopic and zircon age constraints
2009
Abstract The Samothraki mafic suite in the north-eastern Aegean Sea, Greece, is an ‘in situ’ magmatic complex comprising gabbros, sparse dykes and basalt flows and pillows cut by late dolerite dykes. We have determined the age of the complex by SHRIMP zircon geochronology of a gabbro as 159.9 ± 4.5 Ma (i.e. Oxfordian; early Late Jurassic), which precludes any correlation with the so-called Lesvos ophiolite further south (253.1 ± 5.6 Ma; Latest Permian). Six distinct, hitherto unrecognised, geochemical groups have been identified among the basalts and dolerites of the Samothraki mafic suite on the basis of trace-element and Nd–Sr isotopic characteristics. All groups show the presence of an e…
Changes in soil chemical properties as affected by pyrogenic organic matter amendment with different intensity and frequency
2017
Pyrogenic organic matter (PyOM) has long been used as a soil amendment to improve soil physicochemical properties. However, few studies simultaneously investigated both intensities and frequencies of PyOM addition on soil chemical properties of soil base cations, soil pH buffering capacity (pHBC), and plant available micronutrients. In the main food production area of lower Liaohe River Plain in Northeast China, a field manipulation of PyOM addition was initiated in 2013 to examine how the intensities (0, 1%, 3%, and 5% of 0–20 cm soil mass) and frequencies (3% of soil mass applied once versus yearly for 3 years) of PyOM amendment affected soil chemical properties. Higher intensity of PyOM …
Influence of parent material and soil use on arsenic forms in soils A case study in the Amblés Valley (Castilla-León, Spain)
2014
Abstract The total, water soluble and extractable concentrations with EDTA of As from topsoils from the Ambles Valley (Avila, Spain) were determined. The geochemical baseline concentrations of total As were established, and the relationships between the concentration of the different As forms and soil properties were investigated. Total As content in soils was related with parent material, whereas anthropogenic activities affected its mobility. Iron, aluminium, clay content, soil organic matter and soil pH were the main controlling factors for As soil concentrations. The geochemical baseline concentrations obtained (mg kg − 1 ) were 7.3–35 in soils on granite parent material and 2.2–6.8 in …