6533b832fe1ef96bd129a2f5
RESEARCH PRODUCT
Small GTP-binding proteins of the Rho- and Ras-subfamilies are not involved in the actin rearrangements induced by attaching and effacingEscherichia coli
Frank EbelManfred RohdeTrinad ChakrabortyChristoph Von Eichel-streibersubject
Bacterial ToxinsExotoxinsArp2/3 complexmacromolecular substancesShiga ToxinsMicrofilamentMicrobiologyGTP-Binding ProteinsEscherichia coliGeneticsAnimalsHumansActin-binding proteinCytoskeletonMolecular BiologyActinbiologyClostridioides difficileActin remodelingActin cytoskeletonActinsActin CytoskeletonMicroscopy ElectronBiochemistryMicroscopy Electron Scanningras Proteinsbiology.proteinCattleMDia1HeLa Cellsdescription
Attaching and effacing Escherichia coli (AEEC) are extracellular pathogens that induce the formation of actin-rich structures at their sites of attachment to eukaryotic host cells. We analysed whether small GTP-binding proteins of the Rho- and Ras-subfamilies, which control the cellular actin system, are essential for these bacterial-induced microfilament reorganizations. For this purpose we specifically inactivated them using the Clostridium difficile toxins TcdB-10463 and TcdB-1470. Such treatment led to a dramatic breakdown of the normal actin cytoskeleton, but did not abrogate the bacterial-induced actin rearrangements. Our data therefore indicate that the microfilament reorganizations induced by AEEC are independent of those small GTP-binding proteins that under normal conditions control the dynamics and maintenance of the actin cytoskeleton.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1998-06-01 | FEMS Microbiology Letters |