Search results for "Complex"
showing 10 items of 5889 documents
Granulocyte-macrophage colony-stimulating factor-cultured bone marrow-derived macrophages reveal accessory cell function and synthesis of MHC class I…
1988
The antigen-mediated activation of a number of T cell clones by bone marrow (BM) cells cultivated in the presence of various colony-stimulating factor (CSF) preparations was investigated. BM macrophages (BMM phi) grown in L929 cell supernatant as a crude source of macrophage colony-stimulating factor (M-CSF) as well as BM cells propagated in the presence of recombinant M-CSF exhibited transient antigen presentation potential to some T cell clones, being maximal on day 7 and having declined to a low level by day 19 of in vitro culture. Treatment of these long-term-cultivated BMM phi populations with recombinant interferon-gamma (IFN-gamma) resulted in predominant antigen presentation capacit…
Case Management for Patients with Complex Multimorbidity: Development and Validation of a Coordinated Intervention between Primary and Hospital Care
2017
In the past few years, healthcare systems have been facing a growing demand related to the high prevalence of chronic diseases. Case management programs have emerged as an integrated care approach for the management of chronic disease. Nevertheless, there is little scientific evidence on the impact of using a case management program for patients with complex multimorbidity regarding hospital resource utilisation. We evaluated an integrated case management intervention set up by community-based care at outpatient clinics with nurse case managers from a telemedicine unit. The hypothesis to be tested was whether improved continuity of care resulting from the integration of community-based and …
Comparison of antiplatelet effects of aspirin, ticlopidine, or their combination after stent implantation.
1998
Background —This study was performed to analyze the influence of either aspirin, ticlopidine, or their combination on platelet activation and aggregation parameters after stent implantation. Methods and Results —Sixty-one patients with successful implantation of a single Palmaz-Schatz stent in a native coronary artery were randomly assigned to either group A (aspirin 300 mg/d+ticlopidine 2×250 mg/d), group B (ticlopidine 2×250 mg/d), or group C (aspirin 300 mg/d). Platelet activation was evaluated on days 1, 7, and 14 by flow cytometry measurement of expression of CD62p (p-selectin) and the binding of fibrinogen to the platelet surface glycoprotein IIb/IIIa receptor. Platelet aggregation w…
Influence of the terminal complement-complex on reperfusion injury, no-reflow and arrhythmias: a comparison between C6-competent and C6-deficient rab…
1996
Objective: The complement system has been suggested to play a role in reperfusion injury which may result from an enhanced destruction of myocardial tissue or from an impairment of reflow. We investigated the influence of the C5b-9 complement complex on infarct size, reflow and arrhythmogenesis. Methods: Twenty-eight C6-competent rabbits and 18 rabbits with congenital C6 deficiency were subjected to either 30 min or 2 h of coronary artery occlusion followed by reperfusion. C6 deficiency was confirmed by the complement titration test and immunohistology. The triphenyl tetrazolium chloride method was used to delineate infarct size. Reflow into infarcted areas was evaluated histologically afte…
Quality Control for Medicinal Plants
2012
The scientific basis of evidence-based medicine is still poor in herbal medicine. For the integration of herbal medicine into western medicine, internationally accepted standards are necessitated, including quality control of herbal products as well as preclinical and clinical evidence of safety and efficacy. In recent years, thriving innovative technologies emerged in phytotherapy research, e.g. DNA-based technologies for the authentication of plant species, good practice guidelines for standardized experimentations. High-quality herbal materials should undergo rigorous examination by analytical techniques for chemoprofiling of medicinal herbs, as well as toxicological methods to detect co…
Glucose-induced loss of glycosyl-phosphatidylinositol-anchored membrane regulators of complement activation (CD59, CD55) by in vitro cultured human u…
2000
Aims/hypothesis. This study examines whether increased glucose concentrations are responsible for a decreased expression of membrane regulators of complement activation molecules. The effect of high glucose in determining an increase in membrane attack complex deposition on endothelial cells was also investigated. Methods. Endothelial cells were isolated from umbilical cord tissue, cultured in the presence of increased concentrations of glucose, and the expression of CD46, CD55, and CD59 was detected by ELISA (enzyme-linked immunosorbent assay) and by flow cytometry. Glucose-treated endothelial cells were also incubated with antiendothelial cell antibodies and fresh complement to assess the…
Randomized Prospective Study in Patients With Complex Regional Pain Syndrome of the Upper Limb With High-Frequency Spinal Cord Stimulation (10-kHz) a…
2021
Objective The objective of this prospective randomized study of cases and controls was to evaluate the efficacy of treatment with low-frequency spinal cord stimulation (LF-SCS) and 10 kHz spinal cord stimulation (10-kHz SCS) in patients diagnosed with complex regional pain syndrome type I (CRPS) with upper limb involvement. Materials and methods Fifty patients were randomized to receive conventional treatment or SCS with a commercially available low-frequency or 10-kHz system. Patients were assessed at 1, 3, 6, and 12 months. The primary endpoint was at 12-months post permanent implantation of the SCS devices. Outcome measures assessed included: Numerical Rating Scale (NRS), 12-Item Short-F…
Regulation by oestrogens of tachykinin NK3 receptor expression in the rat uterus.
1997
The expression of the tachykinin NK3 receptor and its regulation by ovarian steroids were analyzed by reverse transcription-polymerase chain reaction (RT-PCR) in uteri from ovariectomized rats. A single transcript corresponding to the 325-bp product expected for the tachykinin NK3 receptor was detected in uteri from olive oil-treated (control) ovariectomized rats. The level of tachykinin NK3 receptor mRNA in progesterone-treated animals was similar to that observed in uteri from control ones. Tachykinin NK3 receptor mRNA levels were significantly smaller in uteri from oestrogen-treated ovariectomized rats, with approximately a 32-fold decrease. These findings suggest that oestrogen, but not…
Appropriateness of admission and hospital stay in obstetric wards: an Italian cross-sectional study comparing Sicilian settings
2015
Appropriateness is one of the main topics of health policy and should be estimable in order to analyze the efficacy of hospital services. The Review and Use of the Hospital Protocol (in Italian: Protocollo di Revisione dell’Uso dell’ Ospedale-PRUO) allowed us to evaluate the appropriateness of hospitalizations, starting from daily available clinical records in a selected hospital. Methods The cross-sectional study applied the PRUOmethod on a sample of medical records (N=575; years: 2008–2010) related to ordinary admissions to obstetric wards of Lipari and Pantelleria Hospital (both Minor Islands of Sicily: MI) and to the Teaching Hospital of the University of Palermo (P) in order to evaluat…
PROMOTION OF INTRATHYMIC T-CELL DEVELOPMENT BY MHC-Ia-POSITIVE THYMIC MACROPHAGES (Ia + Mø)
1985
The generation of the peripheral T-cell pool is attributed to maturation and differentiation events occurring within the thymus. Three thymic compartments, thymic epithelial cells, thymic hormons and thymic Mo are considered to contribute to the intrathymic T-cell development. While, as we have shown earlier, murine as well as human MHC-Ia-negative-Mo (Ia−Mo) regulate thymocyte (TH) differentiation via suppressive effects (Thymus,6:295,1984) and MHC-Ia+-Mo are involved in intrathymic tolerance induction (Pediatr Res,15:800, 1984), now the contribution of Ia+Mo to intrathymic lymphopoiesis was investigated. An isolation method yielding cell suspensions highly enriched for Ia+ thymic Mo was p…