Search results for "Complex"
showing 10 items of 5889 documents
Ureteral stent encrustation: evaluation of available scores as predictors of a complex surgery
2022
Background: Stent Encrustation is an uncommon event (13%) with a significant impact in patients' management. Aim of our study was to evaluate the available grading systems for encrusted stents. Methods: A retrospective analysis of encrusted stents was performed in four Italian centers between 2006 and 2020. Encrusted stents were classified according to four classifications: the Forgotten Encrusted Calcificated (FECal) score, the Kidney Ureter Bladder (KUB) score, the Visual Grading for Ureteral Encrusted Stent Classification and the Encrustation Burden Score (EBS). Classifications were evaluated to predict complex surgery defined as: long operative time (>60min); need of more than one su…
PHILOSOPHICAL INVESTIGATIONS ON INTEGRATIVE COMPLEXITY
2019
Integrative complexity (IC) refers to a psychological construct, a measurement, and an experience. As a construct IC refers to our less than conscious thinking style in the face of difference or disagreement, how we process information, make decisions, solve problems. The cross-culturally validated empirical measurement frame of IC has predictive values. ’Low IC’ indicates a simple thinking style that is rigid and closed, a ’tunnel vision’ way of thinking that cannot respect or recognize different dimensions or perspectives on a topic, predicting destructive conflict or violence. An increasingly complex thinking style is able to ’see the big picture’, to …
Variational approach for analysis of harmonic vibration and stabiligy of moving panels
2014
In this paper, the stability of a simply supported axially moving elastic panel (plate undergoing cylindrical deformation) is considered. A complex variable technique and bifurcation theory are applied. As a result, variational equations and a variational principle are derived. Analysis of the variational principle allows the study of qualitative properties of the bifurcation points. Asymptotic behaviour in a small neighbourhood around an arbitrary bifurcation point is analyzed and presented. It is shown analytically that the eigenvalue curves in the (ω, V0) plane cross both the ω and V0 axes perpendicularly. It is also shown that near each bifurcation point, the dependence ω(V0) for each m…
Complex Authority Network Interactions in the Common Information Sharing Environment
2019
European authorities collaborate as a community toward a coherent approach of situational understanding and open trust base information sharing. Innovation in multi-stakeholder collaboration networks involve complex collaboration between user community members, providing cross-sector, cross-border and cross-authority interaction and information sharing for collaborative situation awareness, and cooperation to increase safety and security. This study analyses data consisting of elements of use cases, collected from EU funded innovation projects. These were placed in a table based on similarity, difference and relevance to produce a classification. The results of this study indicate that use …
EFFETTI DELLA PROTEZIONE E DELLA COMPLESSITÀ TOPOGRAFICA SUI POPOLAMENTI BENTONICI DEI REEF A VERMETI
2009
Vermetid reef can be considered a key intertidal habitat of Mediterranean Sea and increasing knowledge about this natural system is important for its conservation. Aims of this study is to assess topography complexity, structure and composition of the benthic assemblages associated to reef formation in a marine protected area highlighting possible effects of the protection.
Binding and internalization of human papillomavirus type 33 virus-like particles by eukaryotic cells
1995
Infection of cells by human papillomaviruses (HPVs) associated with malignant genital lesions has not been studied because of the lack of an in vitro system and the unavailability of virions. We have now used virus-like particles (VLPs) of HPV type 33 to analyze the initial events in the interaction of the HPV capsid with cell lines. Binding of VLPs to HeLa cells was observed in biochemical assays and by immunofluorescence. VLP binding was inhibited by antisera raised against VLPs but not by monoclonal antibodies recognizing either L1 or L2 epitopes accessible on VLPs. Under saturating conditions, approximately 2 x 10(4) VLPs were bound per cell, with a dissociation constant of about 100 pM…
Generation and neutralization of pseudovirions of human papillomavirus type 33
1997
Since human papillomaviruses (HPV) cannot be propagated in cell culture, the generation of infectious virions in vitro is a highly desirable goal. Here we report that pseudovirions can be generated by the assembly of virus-like particles (VLPs) in COS-7 cells containing multiple copies of a marker plasmid. Using recombinant vaccinia viruses, we have obtained spherical VLPs of HPV type 33 (HPV-33) which fractionate into heavy and light VLPs in cesium chloride density gradients. VLPs in the heavy fraction (1.31 g/cm3) carry the plasmid in DNase-resistant form and are capable of transferring the genetic marker located on the plasmid to COS-7 cells in a DNase-resistant way (pseudoinfection). Th…
Viral and cellular determinants of hepatitis C virus RNA replication in cell culture.
2003
Studies on the replication of hepatitis C virus (HCV) have been facilitated by the development of selectable subgenomic replicons replicating in the human hepatoma cell line Huh-7 at a surprisingly high level. Analysis of the replicon population in selected cells revealed the occurrence of cell culture-adaptive mutations that enhance RNA replication substantially. To gain a better understanding of HCV cell culture adaptation, we characterized conserved mutations identified by sequence analysis of 26 independent replicon cell clones for their effect on RNA replication. Mutations enhancing replication were found in nearly every nonstructural (NS) protein, and they could be subdivided into at …
Papillomavirus assembly requires trimerization of the major capsid protein by disulfides between two highly conserved cysteines.
1998
ABSTRACT We have used viruslike particles (VLPs) of human papillomaviruses to study the structure and assembly of the viral capsid. We demonstrate that mutation of either of two highly conserved cysteines of the major capsid protein L1 to serine completely prevents the assembly of VLPs but not of capsomers, whereas mutation of all other cysteines leaves VLP assembly unaffected. These two cysteines form intercapsomeric disulfides yielding an L1 trimer. Trimerization comprises about half of the L1 molecules in VLPs but all L1 molecules in complete virions. We suggest that trimerization of L1 is indispensable for the stabilization of intercapsomeric contacts in papillomavirus capsids.
Human Papillomavirus Type 16 E7 Peptide-Directed CD8+ T Cells from Patients with Cervical Cancer Are Cross-Reactive with the Coronavirus NS2 Protein
2003
ABSTRACTHuman papillomavirus type 16 (HPV16) E6 and E7 oncoproteins are required for cellular transformation and represent candidate targets for HPV-specific and major histocompatibility complex class I-restricted CD8+-T-cell responses in patients with cervical cancer. Recent evidence suggests that cross-reactivity represents the inherent nature of the T-cell repertoire. We identified HLA-A2 binding HPV16 E7 variant peptides from human, bacterial, or viral origin which are able to drive CD8+-T-cell responses directed against wild-type HPV16 E7 amino acid 11 to 19/20 (E711-19/20) epitope YMLDLQPET(T) in vitro. CD8+T cells reacting to the HLA-A2-presented peptide from HPV16 E711-19(20)recogni…