Search results for "Computation"
showing 10 items of 7362 documents
Secondary structure determination of conserved SARS-CoV-2 RNA elements by NMR spectroscopy
2020
AbstractThe current pandemic situation caused by the Betacoronavirus SARS-CoV-2 (SCoV2) highlights the need for coordinated research to combat COVID-19. A particularly important aspect is the development of medication. In addition to viral proteins, structured RNA elements represent a potent alternative as drug targets. The search for drugs that target RNA requires their high-resolution structural characterization. Using nuclear magnetic resonance (NMR) spectroscopy, a worldwide consortium of NMR researchers aims to characterize potential RNA drug targets of SCoV2. Here, we report the characterization of 15 conserved RNA elements located at the 5′ end, the ribosomal frameshift segment and t…
Improving mRNA-Based Therapeutic Gene Delivery by Expression-Augmenting 3' UTRs Identified by Cellular Library Screening.
2019
Synthetic mRNA has emerged as a powerful tool for the transfer of genetic information, and it is being explored for a variety of therapeutic applications. Many of these applications require prolonged intracellular persistence of mRNA to improve bioavailability of the encoded protein. mRNA molecules are intrinsically unstable and their intracellular kinetics depend on the UTRs embracing the coding sequence, in particular the 3′ UTR elements. We describe here a novel and generally applicable cell-based selection process for the identification of 3′ UTRs that augment the expression of proteins encoded by synthetic mRNA. Moreover, we show, for two applications of mRNA therapeutics, namely, (1) …
Polymorphisms of β-defensin genes in Valle del Belice dairy sheep
2011
The aim of this work was to study β-defensin 1 (SBD1) and β-defensin 2 (SBD2) genes in Valle del Belice dairy sheep in order to identify polymorphisms that can be utilized as markers of the analyzed genes, and search for the functional effects and roles of the identified polymorphisms (variation of the amino acid sequence of the protein and stability of mRNA molecule). The study was conducted on 300 randomly selected animals belonging to four flocks. A total of seven SNPs were identified, two in SBD1 and five in SBD2. The two SNPs identified in SBD2 coding region, at position 1659 and position 1667, were non-synonymous, leading to amino acid changes in the protein product. Nevertheless, the…
Design of novel small molecule base-pair recognizers of toxic CUG RNA transcripts characteristics of DM1.
2020
Graphical abstract
Splines Computation by Subdivision
2015
In this chapter, fast stable algorithms are presented, which compute splines’ values at dyadic and triadic rational points starting from their samples at integer grid points. The algorithms are implemented by the causal-anticausal recursive filtering of initial data samples, which is followed by iterated application of FIR filters. Extension of the algorithms to the multidimensional case is straightforward. A natural application of the presented subdivision algorithms is for upsampling of signals and images. A few upsampling examples are provided.
Non-periodic Discrete Splines
2015
Discrete Splines with different spans were introduced in Sect. 3.3.1. This chapter focuses on a special case of discrete splines whose spans are powers of 2. These splines are discussed in more detail. The Zak transform provides an integral representation of such splines. Discrete exponential splines are introduced. Generators of the discrete-spline spaces are described whose properties are similar to properties of polynomial-spline spaces generators. Interpolating discrete splines provide efficient tools for upsampling 1D and 2D signals. An algorithm for explicit computation of discrete splines is described.
Study of USH1 Splicing Variants through Minigenes and Transcript Analysis from Nasal Epithelial Cells
2012
Usher syndrome type I (USH1) is an autosomal recessive disorder characterized by congenital profound deafness, vestibular areflexia and prepubertal retinitis pigmentosa. The first purpose of this study was to determine the pathologic nature of eighteen USH1 putative splicing variants found in our series and their effect in the splicing process by minigene assays. These variants were selected according to bioinformatic analysis. The second aim was to analyze the USH1 transcripts, obtained from nasal epithelial cells samples of our patients, in order to corroborate the observed effect of mutations by minigenes in patient’s tissues. The last objective was to evaluate the nasal ciliary beat fre…
"Table 4" of "Elliptic flow of charged particles in Pb-Pb collisions at 2.76 TeV"
2014
Integrated elliptic flow at sqrt(sNN) = 2.76 TeV for centrality 20-30%.
"Table 208" of "Measurement of the correlation between flow harmonics of different order in lead-lead collisions at $\sqrt{s_{NN}}$=2.76 TeV with the…
2015
RMS eccentricity scaled v_n.
"Table 207" of "Measurement of the correlation between flow harmonics of different order in lead-lead collisions at $\sqrt{s_{NN}}$=2.76 TeV with the…
2015
RMS eccentricity scaled v_n.