Search results for "Confocal Laser Scanning Microscopy"

Immunohistochemical marker for Na+ CP type Vα (C-20) and heterozygous nonsense SCN5A mutation W822X in a sudden cardiac death induced by mild anaphyl…

2009

A sudden death likely due to mild anaphylactic reaction in a young man is described. Autoptic, histologic, immunohistochemical, and laboratory findings were strongly consistent with the diagnosis of a mild anaphylactic reaction. Genetic molecular analysis, performed on formalin-fixed, paraffin-embedded tissues, showed a mutation described as W822X in a family with electrocardiographic pattern typical of Brugada Syndrome. It results in a nonsense mutation generating a truncated form of the channel protein. The mutation is due to a point substitution of a guanine with an adenine residue (G2466A). Immunohistochemistry and laser scanning confocal microscopy on sections from heart formalin-fixed…

Histotomography of the odontoblast processes at the dentine-enamel junction of permanent healthy human teeth in the confocal laser scanning microscope

1998

The translucency of teeth allows the non-destructive subsurface visualisation of their microstructure by confocal laser scanning microscopy (CLSM) at a level of about 150 μm below the surface. The dentine–enamel junction (DEJ) is accessible only directly adjacent to the cervix of the tooth. Therefore teeth have to be sectioned for studying marginal areas of the dental hard tissue. The potential of the technique for (pseudo) three-dimensional visualisation allows the study of an array of individual confocal images, the interpretation of which is similar to that of macroscopic tomographs (CT-scan, MRI). Additionally, the extended focus mode yields the overlay of individual confocal images in …

Strukturelle Veränderungen in säuregeätztem Schmelz im konfokalen Laser-Scanning-Mikroskop

1996

The aim of the present investigation was to elucidate structural alternations in enamel subjected to acid etch technique and treatment under remineralizing conditions by means of a new microscopy technique known as confocal laser scanning microscopy (CLSM). Blocks of enamel were treated with 37% phosphoric acid and exposed for 3 weeks to an oral environment. Tomographic CLSM images were subsequently obtained and compared with controls. CLSM proved to be a reliable, highly reproducible and simple method of qualitative assessment of structural changes occurring on the surface of enamel and in areas below the surface as deep as 100 microns or more. Structural alterations associated with acid a…

Mikromorphometrie und Stereographie des Rauhtiefenreliefs beschichteter Implantatoberflächen durch Rasterelektronenmikroskopie und konfokale Laser-Sc…

1998

Development and viability of biofilms grown on experimental abutments mimicking dental implants: an in vivo model

2019

Background To determine whether an experimental abutment mimicking the macro- and microstructure of a dental implant is a suitable method for recovering biofilm, and to describe the features of biofilms formed around such abutments on healthy implants. Material and Methods Experimental abutments were used in 15 patients without peri-implant diseases. After 14 days’ absence of dental hygiene in this area, the abutments were retrieved and analyzed through confocal laser scanning microscopy and scanning electron microscopy. The biofilm formation on the surface of the first 5 abutments was determined by a fluorescence-staining method using SYTO9 nucleic acid stain. In order to study the biofilm…

Konfokale Laser Scanning Mikroskopie (CLSM) kortikalen Knochens – Vergleichende Darstellung mit konventioneller Mikroskopie

1997

Impact on flavour release of the structure of composite gels containing polysaccharides: Example of the fruit préparations

2006

The main objective of this study was to understand the respective rôle of the structure of a food product and of the physicochemical interactions on flavour release. For that purpose, fruit préparations hâve been chosen as model matrices. Those matrices contain a polysaccharide as gelling agent, a cross-linked waxy corn starch (1.4%), a high sucrose content (35%), an acidic buffer and a strawberry aroma (17 molécules). Four matrices were developed by modifying either the composition or the concentration in gelling agent: two matrices of similar texture containing either LM pectin or carrageenan, and two carrageenan based matrices with différent gelled structures. In a first step; two comple…

Cariostatic effect of a light-cured, resin-reinforced glass-ionomer for bonding orthodontic brackets in vivo

1998

The aim of this study was to evaluate in vivo the cariostatic potential of the resin-reinforced glass-ionomer (Vitremer core build-up restorative; 3M Dental Product Division) when used as a bonding agent for orthodontic brackets. The mineral distribution and topography of the enamel surface adjacent to the bracket base was determined by quantitative microradiography (TMR) and confocal laser scanning microscopy (CLSM). The study was designed in split-mouth technique using 9 pairs of premolars to be extracted for orthodontic reasons. One tooth of each pair was bonded with the resin-reinforced glass-ionomer, and the control contralateral premolar with the non-fluoridated composite (Concise, 3M…

Cavitation corrosion and repassivation kinetics of titanium in a heavy brine LiBr solution evaluated by using electrochemical techniques and Confocal…

2011

The cavitation corrosion behaviour of commercially pure Grade 2 titanium in a 992 g/l LiBr solution has been investigated at 25 °C using an ultrasound device. Cavitation was found to have more influence on the anodic branch than on the cathodic branch, shifting the corrosion potential, Ecorr, and the OCP value towards more negative potentials, and increasing the corrosion current density, icorr, by six times. The repassivation kinetics of Grade 2 titanium have also been studied in the 992 g/l LiBr solution, at 25 °C and various applied potentials, using cavitation to damage the electrode surface. The repassivation kinetics have been analysed in terms of the current density flowing from the …

Exploring the diversity of listeria monocytogenes biofilm architecture by high-throughput confocal laser scanning microscopy and the predominance of …

2015

ABSTRACT Listeria monocytogenes is involved in food-borne illness with a high mortality rate. The persistence of the pathogen along the food chain can be associated with its ability to form biofilms on inert surfaces. While most of the phenotypes associated with biofilms are related to their spatial organization, most published data comparing biofilm formation by L. monocytogenes isolates are based on the quantitative crystal violet assay, which does not give access to structural information. Using a high-throughput confocal-imaging approach, the aim of this work was to decipher the structural diversity of biofilms formed by 96 L. monocytogenes strains isolated from various environments. Pr…