Search results for "Confocal microscopy"
showing 10 items of 120 documents
Confocal technology in fluorescence in situ hybridization evaluation for cancer: a diagnostic improvement.
2013
During the cancer routine diagnosis course, we commonly use fluorescence in situ hybridization (FISH) technique. FISH studies are conducted for genes amplification analyses (ErBb2/Neu) and also for genes translocation studies such as CMYC, BCL6, or BCL2. Usually, FISH evaluation is carried out with fluorescence microscopy and photographed with sensitive cameras. An alternative technology to the fluorescence microscopy is using the confocal microscopy for the evaluation of these samples. Some advantages of confocal microscopy are as follows: First, the use of a laser and pinhole instead of using 511983 IJSXXX10.1177/1066896913511983International Journal of Surgical PathologyCampos et al. res…
Finely Tuned Temperature-Controlled Cargo Release Using Paraffin-Capped Mesoporous Silica Nanoparticles
2011
[EN] Trapped: Mesoporous silica nanoparticles were loaded with a fluorescent guest and functionalized with octadecyltrimethoxysilane. The alkyl chains interact with paraffins, which build a hydrophobic layer around the particle (see picture). Upon melting of the paraffin, the guest molecule is released, as demonstrated in cells for the guest doxorubicin. The release temperature can be tuned by choosing an appropriate paraffin. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Impact of local compressive stress on the optical transitions of single organic dye molecules
2012
The ability to mechanically control the optical properties of individual molecules is a grand challenge in nanoscience and could enable the manipulation of chemical reactivity at the single-molecule level. In the past, light has been used to alter the emission wavelength of individual molecules or modulate the energy transfer quantum yield between them. Furthermore, tensile stress has been applied to study the force dependence of protein folding/unfolding and of the chemistry and photochemistry of single molecules, although in these mechanical experiments the strength of the weakest bond limits the amount of applicable force. Here, we show that compressive stress modifies the photophysical …
Rational assembly and dual functionalization of Au@MnO heteroparticles on TiO2 nanowires
2014
Au–MnO heteroparticles were immobilized on the surface of TiO2 nanowires and tagged subsequently with a fluorescent ligand. The immobilization of the Au@MnO heteroparticles was achieved by functionalizing the TiO2 nanowire support with a polymer containing catechol anchor groups for binding to the metal oxide surface and amine groups for conjugation to the Au domains of the Au@MnO heteroparticles. The Au domain of the resulting TiO2@Au–MnO nanocomposite could be functionalized selectively with a thiol-tagged 24 mer oligomer containing Texas red (SH-ODN-TXS red), whereas a green dye (NBD–Cl) could be anchored selectively to the TiO2 “support” using the free amine groups of the polymeric liga…
Orthogonal functionalisation of upconverting NaYF4 nanocrystals.
2013
A simple and straightforward method for the orthogonal functionalisation of upconverting NaYF4 nanocrystals (UCNCs)-doped withYb(3+) and Er(3+)-based on N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide/N-hydroxysuccinimide (EDC/NHS) selective reactions between two dyes and two different reactive groups present at the periphery of the upconverting nanocrystals is reported. Organic-soluble UCNCs of 10 and 50 nm in size are encapsulated efficiently in a 1:1 mixture of two commercial 3000 Da poly(ethylene glycol) derivatives with two different reactive groups (amino and carboxylic groups). The water-dispersible UCNCs are non-cytotoxic, stable in the physiological environment, and present free ami…
Engineered axon tracts within tubular biohybrid scaffolds
2021
[EN] Injuries to the nervous system that involve the disruption of axonal pathways are devastating to the individual and require specific tissue engineering strategies. Here we analyse a cells-biomaterials strategy to overcome the obstacles limiting axon regeneration in vivo, based on the combination of a hyaluronic acid (HA) single-channel tubular conduit filled with poly-L-lactide acid (PLA) fibres in its lumen, with pre-cultured Schwann cells (SCs) as cells supportive of axon extension. The HA conduit and PLA fibres sustain the proliferation of SC, which enhance axon growth acting as a feeder layer and growth factor pumps. The parallel unidirectional ensemble formed by PLA fibres and SC …
HSP90 and eNOS partially co-localize and change cellular localization in relation to different ECM components in 2D and 3D cultures of adult rat card…
2007
Background information. Cultivation techniques promoting three-dimensional organization of mammalian cells are of increasing interest, since they confer key functionalities of the native ECM (extracellular matrix) with a power for regenerative medicine applications. Since ECM compliance influences a number of cell functions, Matrigel-based gels have become attractive tools, because of the ease with which their mechanical properties can be controlled. In the present study, we took advantage of the chemical and mechanical tunability of commonly used cell culture substrates, and co-cultures to evaluate, on both two- and three-dimensional cultivated adult rat cardiomyocytes, the impact of ECM c…
Radiation effects on silica-based preforms and optical fibers-I: Experimental study with canonical samples
2008
International audience; Prototype samples of preforms and associated fibers have been designed and fabricated through MCVD process to investigate the role of fluorine (F) and germanium (Ge) doping elements on the radiation sensitivity of silica-based glasses. We characterized the behaviors of these canonical samples before, during and after 10 keV X-ray irradiation through several spectroscopic techniques, to obtain global information (in situ absorption measurements, electron paramagnetic resonance) or spatially-resolved information (confocal microscopy, absorption and luminescence on preform). These tests showed that, for the Ge-doped fiber and in the 300–900 nm range, the radiation-induc…
In-vivo-Konfokalmikroskopie in der Diagnose des okulären Schleimhautpemphigoids
2015
Hintergrund: Fur die Prognose des okularen Schleimhautpemphigoids (mucous membrane pemphigoid, MMP) ist eine fruhe Diagnosesicherung essenziell. Allerdings wird die Sensitivitat des sog. diagnostischen Goldstandards, der direkten (DIF) bzw. indirekten Immunfluoreszenz (IIF) mit einer Spanne von 30–80 % sehr variabel angegeben. Zusatzlich birgt die dafur notwendige Bindehautbiopsie die Gefahr der Exazerbation. Ziel dieser Studie war es, zu ermitteln, ob die nicht invasive In-vivo-Konfokalmikroskopie zur Erkennung eines okularen Schleimhautpemphigoids beitragen kann. Patienten und Methoden: Die Konjunktiva von 10 Pemphigoidpatienten und 10 Kontrollprobanden wurden mithilfe des o. g. Verfahren…
Confocal laser endomicroscopy for the diagnosis of urothelial bladder neoplasia: a technology of the future?
2010
Study Type – Diagnostic (exploratory cohort) Level of Evidence 2b What’s known on the subject? and What does the study add? Bladder cancer is initially diagnosed by white light cystoscopy followed by histopathological evaluation after transurethral resection of tissue suspicious for cancer. Difficulties may occur especially in the assessment of flat lesions or in discrimination between CIS and inflammatory disease. Confocal endomicroscopy during diagnostic colonoscopy has been a valuable tool for in vivo microscopic visualization and detection of colonic neoplasias and has contributed to optimized detection rates of up to 99%. We evaluated resected bladder urothelium of 18 patients by confo…