Search results for "Confocal"
showing 10 items of 444 documents
Dye selection for live cell imaging of intact siRNA
2011
Abstract Investigations into the fate of small interfering RNA (siRNA) after transfection may unravel new ways to improve RNA interference (RNAi) efficiency. Because intracellular degradation of RNA may prevent reliable observation of fluorescence-labeled siRNA, new tools for fluorescence microscopy are warranted to cover the considerable duration of the RNAi effect. Here, the characterization and application of new fluorescence resonance energy transfer (FRET) dye pairs for sensing the integrity of duplex siRNA is reported, which allows an assessment of the degradation status of an siRNA cell population by live cell imaging. A panel of high-yield fluorescent dyes has been investigated for …
A New Species of the Genus Crenubiotus (Tardigrada: Eutardigrada: Adorybiotidae) from Salt Spring Island, Strait of Georgia, British Columbia (Canada)
2022
Currently, the recently erected genus Crenubiotus (Adorybiotidae, Macrobiotoidea) includes only three species, all of which are characterised by dentate lunulae and cuticular tubercules organised in the band in the dorso-caudal part of the body. By means of integrative taxonomy, we describe a fourth species of the genus: Crenubiotus salishani sp. nov., from Salt Spring Island in British Columbia, Canada. The new species has been found in the moss growing on rock and differs from the other species in the genus due to the presence of a median anterior mucrone in the third band of the oral cavity armature (OCA) and by the presence of evident thickenings on the eggshell connecting the neighbour…
3D Morphology, ultrastructure and development of Ceratomyxa puntazzi stages: first insights into the mechanisms of motility and budding in the Myxozo…
2012
Free, amoeboid movement of organisms within media as well as substrate-dependent cellular crawling processes of cells and organisms require an actin cytoskeleton. This system is also involved in the cytokinetic processes of all eukaryotic cells. Myxozoan parasites are known for the disease they cause in economical important fishes. Usually, their pathology is related to rapid proliferation in the host. However, the sequences of their development are still poorly understood, especially with regard to pre-sporogonic proliferation mechanisms. The present work employs light microscopy (LM), electron microscopy (SEM, TEM) and confocal laser scanning microscopy (CLSM) in combination with specific…
ANTIMICROBIAL AND ANTISTAPHYLOCOCCAL BIOFILM ACTIVITY FROM THE SEA URCHIN PARACENTROTUS LIVIDUS
2009
Aims: Staphylococcal biofilm-associated infections are resistant to conventional antibiotics. Consequently, new agents are needed to treat them. With this aim, we focused on the effector cells (coelomocytes) of the sea urchin Paracentrotus lividus immune system. Methods and Results: We tested the activity of the 5-kDa peptide fraction of the cytosol from coelomocytes (5-CC) against a group of Gram-positive, Gram-negative bacteria and fungi. We determined minimal inhibitory concentrations (MICs) ranging from 253.7 to 15.8 mg ml(-1). We observed an inhibitory activity and antibiofilm properties of 5-CC against staphylococcal biofilms of reference strains Staphylococcus epidermidis DSM 3269 an…
Evaluating the microscopic effect of brushing stone tools as a cleaning procedure
2020
Cleaning stone tool surfaces is a common procedure in lithic studies. The first step widely applied at any archeological site (and/or at field laboratories) is the gross removal of sediment from the surfaces of artifacts. Lithic surface alterations due to mechanical action applied in wet or dry cleaning regimes have never been examined at a microscopic scale. This could have important implications in traceology, as any modern surface modifications inflicted on archeological artifacts might compromise their functional interpretations. The current trend toward quantification of use-wear traces makes the testing even more important, as even slight, apparently invisible surface alterations migh…
Strategie di difesa in risposta a stress in embrioni di riccio di mare: L'embrione di riccio di Paracentrotus lividus come modello sperimentale per l…
2013
Negli ultimi decenni, grazie alle nuove tecnologie e alla maggiore disponibilità di strumenti di ricerca, il campo riguardante lo studio delle strategie di difesa attivate nella risposta a stress, compresi i meccanismi di apoptosi e autofagia, è divenuto molto ampio ed è in continua espansione. Sin dagli albori della Biologia, gli studi condotti sul modello sperimentale di riccio di mare hanno permesso di ottenere conoscenze di base, spesso applicabili ad altri modelli biologici. I dati riportati nel presente libro dovrebbero aiutare a far luce sui meccanismi di difesa che le cellule sono in grado di attivare, in caso di necessità, se esposte a sfavorevoli condizioni ambientali. Le osservaz…
Piezospectroscopic study of mechanical stress in Al2O3:Cr under swift heavy ion irradiation
2009
Abstract The spatial distribution of mechanical stresses in Al2O3:Cr single crystal irradiated with (1 ÷ 3) MeV/amu Kr, Xe and Bi ions has been studied by using laser confocal scanning microscopy technique. The stress level as a function of the ion penetration depth has been evaluated from depth-resolved photostimulated R-line luminescence spectra exploiting the piezospectroscopic method. As it was found, the stress field generated by swift heavy ion irradiation is composed of stresses with maximal magnitude comparable with the ultimate stress limit of ruby crystals. Experimental data are discussed in the framework of a model considering the Cr3+ atoms as individual piezosensors.
Surpassing digital holography limits by lensless object scanning holography.
2012
We present lensless object scanning holography (LOSH) as a fully lensless method, capable of improving image quality in reflective digital Fourier holography, by means of an extremely simplified experimental setup. LOSH is based on the recording and digital post-processing of a set of digital lensless holograms and results in a synthetic image with improved resolution, field of view (FOV), signal-to-noise ratio (SNR), and depth of field (DOF). The superresolution (SR) effect arises from the generation of a synthetic aperture (SA) based on the linear movement of the inspected object. The same scanning principle enlarges the object FOV. SNR enhancement is achieved by speckle suppression and c…
Intrahepatic myeloid-cell aggregates enable local proliferation of CD8+T cells and successful immunotherapy against chronic viral liver infection
2013
Chronic infection is difficult to overcome because of exhaustion or depletion of cytotoxic effector CD8(+) T cells (cytotoxic T lymphoytes (CTLs)). Here we report that signaling via Toll-like receptors (TLRs) induced intrahepatic aggregates of myeloid cells that enabled the population expansion of CTLs (iMATEs: 'intrahepatic myeloid-cell aggregates for T cell population expansion') without causing immunopathology. In the liver, CTL proliferation was restricted to iMATEs that were composed of inflammatory monocyte-derived CD11b(+) cells. Signaling via tumor-necrosis factor (TNF) caused iMATE formation that facilitated costimulation dependent on the receptor OX40 for expansion of the CTL popu…
The actin-based motility of intracellularListeria monocytogenesis not controlled by small GTP-binding proteins of the Rho- and Ras-subfamilies
1999
In this study, we analyzed whether the actin-based motility of intracellular Listeria monocytogenes is controlled by the small GTP-binding proteins of the Rho- and Ras-subfamilies. These signalling proteins are key regulatory elements in the control of actin dynamics and their activity is essential for the maintenance of most cellular microfilament structures. We used the Clostridium difficile toxins TcdB-10463 and TcdB-1470 to specifically inactivate these GTP-binding proteins. Treatment of eukaryotic cells with either of these toxins led to a dramatic breakdown of the normal actin cytoskeleton, but did not abrogate the invasion of epithelial cells by L. monocytogenes and had no effect on …