Search results for "Coplas"

Las tristes reinas de Nápoles

1972

Synthesis, computational docking and biological evaluation of celastrol derivatives as dual inhibitors of SERCA and P-glycoprotein in cancer therapy.

2021

Abstract A series of eleven celastrol derivatives was designed, synthesized, and evaluated for their in vitro cytotoxic activities against six human cancer cell lines (A549, HepG2, HepAD38, PC3, DLD-1 Bax-Bak WT and DKO) and three human normal cells (LO2, BEAS-2B, CCD19Lu). To our knowledge, six derivatives were the first example of dipeptide celastrol derivatives. Among them, compound 3 was the most promising derivative, as it exhibited a remarkable anti-proliferative activity and improved selectivity in liver cancer HepAD38 versus human normal hepatocytes, LO2. Compound 6 showed higher selectivity in liver cancer cells against human normal lung fibroblasts, CCD19Lu cell line. The Ca2+ mob…

Determination of milk production losses in Valle del Belice sheep following experimental infection of Mycoplasma agalactiae

2015

Abstract Economic losses due to contagious agalactia (CA) in small ruminant herds are mainly associated with significant reductions in or complete loss of dairy production, mortality, abortions, ill thrift, early culling and costs of control. With the aim of estimating milk production losses caused by CA, 46 primiparous lactating Valle del Belice ewes were monitored after experimental infection. Sixty days after lambing, two ewes were each experimentally infected with a single dose of 10 8  CFU/ml of a live Mycoplasma agalactiae strain in both teats by intracanalicular route. Three days after inoculation, the infection was spread manually by the milkers dipping their hands in the pooled mil…

Single tube real time PCR for detection of Streptococcus pneumoniae, Mycoplasma pneumoniae, Chlamydophila pneumoniae and Legionella pneumophila from …

2012

We designed a multiplex real time PCR for rapid, sensitive and specific detection of Streptococcus pneumoniae, Legionella pneumophila, Chlamydophila pneumoniae and Mycoplasma pneumoniae. The study cases consisted of 129 patients with community acquired pneumonia (CAP). Bacteriological techniques were implemented for detection of the cultivable organisms. DNA were extracted from sputa, throat swabs, bronchoalveolar lavages and tracheal aspirates and used as templates in real time PCR. The primers and probes were designed for cbpA (S. pneumoniae), p1adhesin (M. pneumoniae), mip (L. pneumophila) and ompA (C. pneumoniae). After optimization of real time PCR for every organism, the experiments w…

INFEZIONE DA MICOPLASMA ED ANEMIA EMOLITICA: DA TRIGGER AD INDUTTORE DI AUTOIMMUNITA'

2011

MYCOPLASMA PNEUMONIAE INFECTIONS IN PAEDIATRIC AGE: CLINICAL VARIABILITY

2013

Mycoplasma fermentans-derived lipid inhibits class II major histocompatibility complex expression without mediation by interleukin-6, interleukin-10,…

1996

Mycoplasma cause several diseases in man and animals. Some strains can chronically infect humans, leading to fever or inflammatory syndromes such as arthritis, particularly in immunosuppressed patients. A set of pathogenicity factors shared by many mollicutes may be membrane components that activate macrophages to secrete cytokines and other inflammatory mediators. Mycoplasma-derived high molecular weight material (MDHM) is a macrophage-activating amphiphilic lipid which was purified from Mycoplasma fermentans. We studied the influence of MDHM on the expression of major histocompatibility complex (MHC) class II molecules by mouse resident peritoneal macrophages with an ELISA. Highly purifie…

Nonstationary flow surface theory for modeling the viscoplastic behaviors of soils

2016

Abstract This paper presents a three-dimensional elastic viscoplastic model that can describe the time-dependent behaviors of soft clays. The constitutive model is formulated based on the nonstationary flow surface theory and incorporates new developments, including (i) an improved definition of the nonstationary flow surface that is capable of capturing the stress–strain behaviors under different loading paths, (ii) a unique stress–strain—viscoplastic-strain-rate equation that is able to explicitly describe the nonstationary flow surface, and (iii) a final stable state concept that identifies the final equilibrium state at the end of creep and stress relaxation, which is also used to simpl…

A comparison of the efficacy of commercial and experimental vaccines for contagious agalactia in sheep

2013

Abstract An effective vaccine that improves the control of contagious agalactia in Mediterranean regions would be beneficial for poorer farmers who use traditional husbandry methods for sheep and goat production. However while there is little published data on the efficacy of commercially available vaccines for contagious agalactia, evidence from the field suggests that some provide inadequate protection. This paper compares four different vaccine formulations, including a widely used commercial vaccine, using clinical signs and mycoplasma excretion as measures of protection in sheep. Results showed that protection afforded by the vaccines, following contact challenge with experimentally in…

Validation of Loop-Mediated Isothermal Amplification (LAMP) Field Tool for Rapid and Sensitive Diagnosis of Contagious Agalactia in Small Ruminants

2020

Simple Summary Contagious agalactia (CA) is an infectious disease of small ruminants endemic in the Mediterranean countries, causing significant socioeconomic impacts predominantly on small-scale farmers who still subsist on marginal lands. Mycoplasma agalactiae is historically considered the principal etiological agent of CA, especially in sheep. Clinical signs are characterised by mastitis, arthritis, keratoconjunctivitis and occasionally, abortion. Rapid, accurate and cost-effective field tests are urgently needed for effective control of M. agalactiae mastitis. Our study illustrated the validation of a Loop-Mediated Isothermal Amplification (LAMP) test for the detection of M. agalactiae…