Search results for "Cruciform"
showing 6 items of 6 documents
TWJ-Screen: an isothermal screening assay to assess ligand/DNA junction interactions in vitro
2017
International audience; The quest for chemicals able to operate at selected genomic loci in a spatiotemporally controlled manner is desirable to create manageable DNA damages. Mounting evidence now shows that alternative DNA structures, including G-quadruplexes and branched DNA (or DNA junctions), might hamper proper progression of replication fork, thus triggering DNA damages and genomic instability. Therefore, small molecules that stabilize these DNA structures are currently scrutinized as a promising way to create genomic defects that cannot be dealt with properly by cancer cells. While much emphasis has been recently given to G-quadruplexes and related ligands, we report herein on three…
Engineering a 2D protein-DNA crystal.
2005
(Figure Presented) Weaving with DNA: A DNA-binding protein was used to control the structure of a self-assembled 2D crystal. In the absence of protein, four oligonucleotides hybridize to form a Kagome lattice of interwoven double helices with p3 symmetry (see image). Addition of protein RuvA during assembly changes the symmetry and connectivity to give a DNA-protein crystal with an approximately square unit cell. © 2005 Wiley-VCH Verlag GmbH and Co. KGaA.
A natural A/T-rich sequence from the yeast FBP1 gene exists as a cruciform in Escherichia coli cells.
1993
Abstract Palindromic or semipalindromic sequences can adopt cruciform structures in DNA in vitro. It has been demonstrated in some cases that A/T-rich cruciforms exist also in vivo in Escherichia coli. The biological function of those structures is not understood although putative cruciforms have been found in interesting locations on replication origins, operators, or transcriptional termination regions. Here we show by means of the use of structure-dependent nucleases that the 3′ end of the yeast FBP1 gene contains a stable cruciform both in vitro and in E. coli cells and that in both cases, its extrusion depends on the DNA supercoiling state.
Effects of shape of transverse cross-section on the load carrying capacity of laminated glass columns
2014
Abstract In this paper simplified expressions for the calculus of the load carrying capacity of glass columns having rectangular, tee (T) and cruciform (X) transverse cross-sections are proposed and verified against available experimental data. Members with T and X cross-sections are obtained by gluing single multilayered glass panels. The expressions derived take into account of the: shape of the transverse cross-section (rectangular, T, and, X sections); buckling phenomenon (flexural and torsional); failure in the glued sections; long time effects. Case of full connections and absence of connections are considered to determine the range of variation of expressions adopted. The comparison …
Fracture Plane of Cruciform Specimen in Biaxial Low Cycle Fatigue—Estimate by Variance Method and Experimental Verification
1995
This paper presents the variance method of determining the fracture plane under random multiaxial stress states. The fracture plane was estimated analytically by the variance method with the three fatigue criteria. The estimated fracture planes were compared with experimental results using type SUS 304 and 1Cr-1Mo-1/4V steel cruciform specimens. The variance method with the maximum normal strain criterion, by neglecting the strain in direction in which no external forces act, could estimate the actual fracture planes of cruciform specimens in high temperature biaxial low cycle fatigue.
Cruciform Electron Acceptors Based on Tetraindeno-Fused Spirofluorene
2017
Two cruciform tetraindenospirofluorene-based acceptors embedding carbonyl (Spiro-4O) and dicyanovinylene (Spiro-8CN) functionalities are synthesized in high yields. Single-crystal X-ray analysis reveals a one-dimensional π–π stacking arrangement for Spiro-4O, while Spiro-8CN adopts a unique two-dimensional isotropic π-interaction. Cyclic voltammetry suggests a high electron affinity of −3.76 eV for Spiro-8CN. Such a packing motif and low LUMO energy for Spiro-8CN are important for bulk electron transport.