Search results for "Cuba."
showing 10 items of 340 documents
Resistance of a recombinant Escherichia coli to dehydration.
2009
International audience; Dehydration of microorganisms, rendering them anhydrobiotic, is often an efficient method for the short and long term conservation of different strain-producers. However, some biotechnologically important recombinant bacterial strains are extremely sensitive to conventional treatment. We describe appropriate conditions during dehydration of the recombinant Escherichia coli strain HB 101 (GAPDH) that can result dry cells having a 88% viability on rehydration. The methods entails air-drying after addition of 100 mM trehalose to the cultivation medium or distilled water (for short term incubation).
Nest Insulating Capacity during Incubation and after Fledging are Related
2016
Most birds build nests to hold eggs and nestlings. An important property of nests is their ability to keep eggs and nestlings at an optimum temperature. This is usually measured as the insulating capacity (IC); nests with a higher IC will keep their content warm for longer. The usual protocol to estimate IC involves collecting nests after fledging of the young. However, nest properties change throughout the nesting period, potentially affecting IC. Therefore, a relevant question is whether the nest IC, measured after fledging, actually reflects its IC during incubation and early nestling development, when it is most crucial. In April 2015, we collected 18 Great Tit ( Parus major) nests 3-4…
The role of partial incubation and egg repositioning within the clutch in hatching asynchrony and subsequent effects on breeding success
2019
The main mechanism to achieve hatching asynchrony (HA) for incubating birds is to start heating the eggs before clutch completion. This might be achieved through partial incubation and/or early incubation. Even in the absence of incubation behaviour during the laying phase, clutches still experience a certain degree of asynchrony. Recent studies have shown that eggs located in the centre of the nest receive more heat than peripheral ones during incubation. As eggs receiving more heat would develop faster, we hypothesized that HA should be shorter in nests where eggs were moved homogeneously along the centre–periphery space during incubation than in those nests where eggs repeatedly remained…
Biochemical Quality of Crop Residues and Carbon and Nitrogen Mineralization Kinetics under Nonlimiting Nitrogen Conditions
2000
International audience; Statistical relationships were established between the fate of C and N from 47 types of crop residues and their biochemical characteristics during a soil incubation at 15°C. The incubations were carried out under nonlimiting N in order to differentiate the effects of biochemical characteristics of residues from those of soil N availability. Depending on the residue, the apparent mineralization of residue C after 168 d varied from 330 to 670 g kg−1 of added C. Mineralization kinetics were described using a two-compartment decomposition model that decomposes according to first-order kinetics. Amounts of C mineralized after 7 d and the decomposition rate coefficient of …
Discriminating uniparental and biparental breeding strategies by monitoring nest temperature
2017
10 pages; International audience; Birds exhibit a wide diversity of breeding strategies. During incubation or chick-rearing, parental care can be either uniparental, by either the male or the female, or biparental. Understanding the selective pressures that drive these different strategies represents an exciting challenge for ecologists. In this context, assigning the type of parental care at the nest (e.g. biparental or uniparental incubation strategy) is often a prerequisite to answering questions in evolutionary ecology. The aim of this study was to produce a standardized method unequivocally to assign an incubation strategy to any Sanderling Calidris alba nest found in the field by moni…
Predicting the invasion of the acoustic niche: Potential distribution and call transmission efficiency of a newly introduced frog in Cuba
2021
8 pages; International audience; Leptodactylus fragilis is a recently introduced frog in Cuba, where it may impact local populations of amphibians in different ways. Here, we combined two methods to predict the invasion of the acoustic niche of Cuban amphibians by L. fragilis. We first use species distribution models to predict the spread and establishment of L. fragilis in Cuba. We then performed sound propagation experiments to evaluate the potential invasion of the acoustic niche in predicted suitable areas for the presence of L. fragilis. This species could have a successful establishment, spreading mainly in open areas, where its advertisement calls propagate efficiently, with low atte…
Influence of warming temperatures on coregonine embryogenesis within and among species
2021
The greatest response of lakes to climate change has been the increase in water temperatures on a global scale. The responses of many lake fishes to warming water temperatures are projected to be inadequate to counter the speed and magnitude of climate change, leaving some species vulnerable to decline and extinction. We experimentally evaluated the responses of embryos from a group of cold, stenothermic fishes (Salmonidae Coregoninae) - within conspecifics across lake systems, between congeners within the same lake system, and among congeners across lake systems - to a thermal gradient using an incubation method that enabled global comparisons. Study groups included cisco (Coregonus artedi…
Exploring how social interactions influence regulators and innovators: The case of regulatory sandboxes
2020
Like incubators, regulatory sandboxes constitute a prominent mechanism to enable entrepreneurial activities that guide financial technology (FinTech) firms through regulatory frameworks in the financial industry. Because they are new, there is a lack of research on regulatory sandboxes; most studies have investigated legal aspects while overlooking the management perspective. To address this gap, this paper builds on incubation research studies to explore how social interactions within regulatory sandboxes influence the practices of regulators and regulatees, using social capital theory. An exploratory-abductive approach is adopted, using data collected from 16 semi-structured interviews. T…
Impact of Green Vegetables and Green Smoothies on Enamel Mineral Composition In Vitro
2019
Background: Recently, smoothies with a high vegetable content have become very popular, especially among young adults. Objectives: This in vitro study was conducted to determine the erosive potential of selected green vegetables and green smoothies on human dental enamel. Methods: A total of ten green smoothies and eight freshly mixed vegetables were chosen, and pH, calcium and oxalic acid contents were measured. The enamel specimens were obtained from wisdom teeth and incubated with 3 selected foods (spinach, parsley, or green smoothie) for 12 and 24 hours (N = 6). Control samples were incubated with a physiological salt solution. An electron probe microanalyzer (Jeol JXA 8900RL) was utili…
Novel noninvasive embryo selection algorithm combining time-lapse morphokinetics and oxidative status of the spent embryo culture medium.
2019
Objective To develop a noninvasive embryo selection algorithm consisting of time-lapse morphokinetics and the oxidative status of the spent embryo culture medium determined using the Thermochemiluminescence (TCL) Analyzer. Design Retrospective cohort. Setting Not applicable. Patient(s) From women participating in the oocyte donation program, data from 505 samples of spent embryo culture media samples from 292 intracytoplasmic sperm injection cycles. Intervention(s) None. Main Outcome Measure(s) Morphokinetic parameters assessed during incubation in the time-lapse system Embryoscope. Oxidative parameters (H1sm, H2sm, and H3sm) from the spent culture medium on day 5 of incubation measured usi…