Search results for "Cucumovirus"
showing 3 items of 3 documents
Arabidopsis SGS2 and SGS3 genes are required for posttranscriptional gene silencing and natural virus resistance.
2000
AbstractPosttranscriptional gene silencing (PTGS) in plants results from the degradation of mRNAs and shows phenomenological similarities with quelling in fungi and RNAi in animals. Here, we report the isolation of sgs2 and sgs3 Arabidopsis mutants impaired in PTGS. We establish a mechanistic link between PTGS, quelling, and RNAi since the Arabidopsis SGS2 protein is similar to an RNA-dependent RNA polymerase like N. crassa QDE-1, controlling quelling, and C. elegans EGO-1, controlling RNAi. In contrast, SGS3 shows no significant similarity with any known or putative protein, thus defining a specific step of PTGS in plants. Both sgs2 and sgs3 mutants show enhanced susceptibility to virus, d…
Glucose, fructose and saccharose metabolism in Dipsacus silvestris infected by Cucumber mosaic virus.
2007
Dipsacus silvestris Miller (or Fuller’s Teasel) (Dipsacaceae) showing stunting, chlorotic leaf mosaic and narrowing of the lamina, has been found for the first time in Italy naturally infected by Cucumber mosaic virus (CMV). This virus was detected in PAS-ELISA tests and molecularly characterized by applying RT-PCR, using specific primers for the movement protein gene of RNA3 (forward 5’ CTA GGC TTT CCA AGC TAC CAG 3’; reverse 5’ CTA AAG ACC GTT AAC CAC CTG C 3’). The content of glucose, fructose and saccharose, determined by HPLC analysis, was calculated in both healthy (h) and CMV-infected (i) D. silvestris plants during the chronic infection period. An higher content of these carbohydrat…
Population genetics of cucumber mosaic virus infecting medicinal, aromatic and ornamental plants from northern Italy
2012
An epidemiological survey carried at the Herb Garden of Casola Valsenio (Emilia Romagna region; Northern Italy) from 2006 to 2009 resulted in finding Cucumber mosaic virus (CMV) as the infectious agent of several ornamental, medicinal and aromatic plants. All CMV-infected plant species showed leaf symptoms followed, in some cases, by flower colour-breaking and stunting. Genetic analysis consisted in the characterization of movement protein gene of each CMV-isolate by single strand polymorphism analysis and sequencing. Phylogenetic trees identified only two isolates as member of subgroup II, while all other isolates were part of subgroup IA. This survey confirms an unexpected increment of CM…