Search results for "Culture"
showing 10 items of 11608 documents
Co-infection with iflaviruses influences the insecticidal properties of Spodoptera exigua multiple nucleopolyhedrovirus occlusion bodies: Implication…
2017
Biological insecticides based on Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) can efficiently control S. exigua larvae on field and greenhouse crops in many parts of the world. Spanish wild populations and laboratory colonies of S. exigua are infected by two iflaviruses (SeIV-1 and SeIV-2). Here we evaluated the effect of iflavirus co-infection on the insecticidal characteristics of SeMNPV occlusion bodies (OBs). Overall, iflavirus co-inoculation consistently reduced median lethal concentrations (LC50) for SeMNPV OBs compared to larvae infected with SeMNPV alone. However, the speed of kill of SeMNPV was similar in the presence or absence of the iflaviruses. A reduction of the we…
Whole-genome resequencing of Cucurbita pepo morphotypes to discover genomic variants associated with morphology and horticulturally valuable traits
2019
[EN] Cucurbita pepo contains two cultivated subspecies, each of which encompasses four fruit-shape morphotypes (cultivar groups). The Pumpkin, Vegetable Marrow, Cocozelle, and Zucchini Groups are of subsp. pepo and the Acorn, Crookneck, Scallop, and Straightneck Groups are of subsp. ovifera. Recently, a de novo assembly of the C. pepo subsp. pepo Zucchini genome was published, providing insights into its evolution. To expand our knowledge of evolutionary processes within C. pepo and to identify variants associated with particular morphotypes, we performed whole-genome resequencing of seven of these eight C. pepo morphotypes. We report for the first time whole-genome resequencing of the four…
Fatty acids produced by Neofusicoccum vitifusiforme and N. Parvum, fungi associated with grapevine botryosphaeria dieback
2018
There is evidence that secondary metabolites are involved in the fungal pathogenicity and virulence of Neofusicoccum spp. Fatty acids may also influence the plant&ndash
Gametic embryogenesis through isolated microspore culture in Corylus avellana L.
2015
Haploid technology is a valuable plant breeding tool for obtaining homozygosity particularly in woody plants. Hazelnut, the world’s sixth ranking nut tree crop is a monoecious, anemophilous species. It is characterized by a sporophytic incompatibility system that prevents production of homozygous plants with conventional methods, involving several self-pollination cycles. In this study, gametic embryogenesis, in particular isolated microspore culture, was tried with five genotypes. Two culture media were tested and four temperature stress treatments were applied to the isolated microspores that were cultivated at the vacuolated developmental stage. To our knowledge, this is the first report…
Gaining Insight into Exclusive and Common Transcriptomic Features Linked with Biotic Stress Responses in Malus
2017
Identifying key information in transcriptomic data is very important, especially when the “omic” study deals with plant responses to stresses in field conditions where a high number of variables and disturbing factors may affect the analysis. In this meta-analysis we collected 12 transcriptomic works in Malus in order to identify which key genes, proteins, gene categories are involved in general plant pathological conditions and those features linked with exclusive biotic stress responses. Those genes that are only related with molecular responses to pathogen attacks and those linked with other plant physiological processes were identified. A pipeline composed by pathway and gene set enrich…
Biotechnological Applications for the Sustainable Use of Marine By-products: In Vitro Antioxidant and Pro-apoptotic Effects of Astaxanthin Extracted …
2019
In this study, the carotenoid astaxanthin was obtained by supercritical fluid extraction (SFE) from shrimp by-products (SBP). Its bioactive properties were evaluated in vitro in human normal and cancerous cells lines. The antioxidant activity of the extracted astaxanthin of the SFE fraction (ASTA) was tested in fibroblast cells (HS-68), by inducing oxidative stress and by evaluating the protective effect of the pre-treatment with different levels of ASTA against toxicity. The anti-proliferative activity was evaluated in hepatoma cells (HEP-G2), treated with increased concentrations of ASTA and measuring the effects on vitality and on some biomolecular markers related to oxidative stress, ce…
Intestinal parasites of unisexual and bisexual lizards Darevskia spp. (Lacertidae) from Northeastern Anatolia
2016
Summary Four bisexual and two unisexual species of the lizard genus Darevskia from northeastern Anatolia were searched for intestinal parasites in adult specimens. One cestode, Nematotaenia tarentolae, and two nematode species, Spauligodon saxicolae and Strongyloides darevskyi, were found, the latest identified as a Darevskia specialist. No major differences between host species were recorded. The very low infection rates and diversity result in depauperate helminth communities for all these lacertid lizards being the lowest among the Palaearctic saurians. Patterns of these helminth communities are compared with those observed in other lacertid lizards from Anatolia and Europe.
Microspore Embryogenesis in Almond (Prunus dulcis Mill.)
2018
Almond is the most important tree nut crop in terms of commercial production. Its production is limited to areas characterized by a Mediterranean climate, including regions in the Mediterranean countries, the Central Valley of California, Central Asia, the Himalayan slopes and some equivalent areas in the Southern Hemisphere, including Argentina, Australia and South Africa (Kester et al. 1975).
Twig and Shoot Dieback of Citrus, a New Disease Caused by Colletotrichum Species
2021
(1) Background: This study was aimed at identifying the Colletotrichum species associated with twig and shoot dieback of citrus, a new syndrome occurring in the Mediterranean region and also reported as emerging in California. (2) Methods: Overall, 119 Colletotrichum isolates were characterized. They were recovered from symptomatic trees of sweet orange, mandarin and mandarin-like fruits during a survey of citrus groves in Albania and Sicily (southern Italy). (3) Results: The isolates were grouped into two distinct morphotypes. The grouping of isolates was supported by phylogenetic sequence analysis of two genetic markers, the internal transcribed spacer regions of rDNA (ITS) and β-tubulin …
Effects of plant density and cutting-type on rooting and growth of an extensive green roof of Sedum sediforme (Jacq.) Pau in a Mediterranean environm…
2020
Abstract Species of the Sedum genus are a popular choice for green roofs as they thrive in shallow growth layers and are resilient to extreme environmental conditions; they are also easy to propagate from seed, plantlings or plant parts. The use of rooted cuttings is most widespread due to good rates of establishment. In the interest of cost reduction, the direct spreading of Sedum cuttings over the roof area, especially over large areas, has attracted attention in recent years. Considering the interest on the above method and the seeming lack of experimental data available, this study aims to further knowledge in this area. Experimental tests on direct spreading of unrooted cuttings of Sed…