Search results for "Cyclin"

Reconstitution of vesicular transport to Rab11-positive recycling endosomes in vitro.

2003

Rab GTPases are key regulators of vesicular protein transport in both the endocytic and exocytic pathways. In endocytosis and recycling, Rab11 plays a role in receptor recycling to plasma membrane via the pericentriolar recycling compartment. However, little is known about the molecular requirements and partners that promote transport through Rab11-positive recycling endosomes. Here, we report a novel approach to reconstitute transport to immunoabsorbed recycling endosomes in vitro. We show that transport is temperature-, energy-, and time-dependent and requires the presence of Rab proteins, as it is inhibited by the Rab-interacting protein Rab GDP-dissociation inhibitor that removes Rab pr…

Novel therapeutic targets in esophageal cancer: impact of chemokine receptor CXCR4

2007

Ines Gockel†, Carl C Schimanski, Markus Moehler & Theodor Junginger †Author for correspondence Johannes GutenbergUniversity of Mainz, Department of General and Abdominal Surgery, Langenbeckstr. 1, 55131 Mainz, Germany Tel.: +49 6131 177 291; Fax: +49 6131 176 630; gockel@ach.klinik.unimainz.de ‘The interaction between esophageal cancer-expressed CXCR4 and SDF-1α may have a key role in directing malignant cells to ‘homing’ organs ... thus, this mechanism may account for metastasis.’

Macrophage migration inhibitory factor is critically involved in basal and fluoxetine-stimulated adult hippocampal cell proliferation and in anxiety,…

2011

Intensive research is devoted to unravel the neurobiological mechanisms mediating adult hippocampal neurogenesis, its regulation by antidepressants, and its behavioral consequences. Macrophage migration inhibitory factor (MIF) is a pro-inflammatory cytokine that is expressed in the CNS, where its function is unknown. Here, we show, for the first time, the relevance of MIF expression for adult hippocampal neurogenesis. We identify MIF expression in neurogenic cells (in stem cells, cells undergoing proliferation, and in newly proliferated cells undergoing maturation) in the subgranular zone of the rodent dentate gyrus. A causal function for MIF in cell proliferation was shown using genetic (M…

Economía informal, chatarreo y marco social : reflexiones a partir del caso de los gitanos rumanos en Valencia (España)

2023

Este artículo aborda el chatarreo y otras actividades económicas informales realizadas por los gitanos rumanos en Valencia, con un triple objetivo. Uno, analizar el chatarreo como actividad económica informal de reciclaje y las estrategias y los recursos utilizados. Dos, captar las implicaciones del chatarreo y de su valoración social en la situación del colectivo. Tres, conocer los principales factores que establecen diferencias entre la situación en Valencia y otras ciudades europeas occidentales, con algunas poblaciones del sur global con experiencias más positivas para los recicladores informales. Basado en una metodología cualitativa, con trabajo de campo realizado entre septiembre de …

Design and development of a new machine tool for continuous friction stir extrusion

2023

In the present paper the Continuous Friction Stir Extrusion process (CFSE), derived from the already known Friction Stir Extrusion (FSE), is proposed for effective solid-state recycling of metal scraps. A new dedicated machine tool was designed and developed. A Lagrangian implicit thermomechanical numerical model was set up and used to determine the proper ranges of the most relevant process parameters. An effective case study is proposed, involving the actual extrusion of a recycled wire in a continuous way. Metallurgical observations and mechanical characterization tests were carried out on the obtained specimen showing the potential of the process and its industrial feasibility.(c) 2023 …

Preparation and Recycling of Plasticized PLA

2011

Breaking the siege. From the paleo- to the neo-anthropocene

2017

Il contributo descrive in che modo e con quali strumenti di progetto, la città può essere ripensata alla luce della presa di coscienza della rilevanza della Città e dell'Uomo nell'evoluzione del Pianeta. I punti posti in attenzione sono: la costruzione di nuovi cicli vitali per la città, la rilevanza del tempo, la definizione di nuove priorità di intervento, l'uso degli scarti. The paper describes how and with what project tools the city can be re-thought in the light of the awareness of the importance of the City and of Man in the evolution of the Planet. The points of attention are: 1. building new life cycles for the city as a new way of designing the future of our cities and not just as…

Recycling and Thermomechanical Degradation of LDPE/Modified Clay Nanocomposites

2014

Regulation of Endurance Performance: New Frontiers

2017

Successful endurance performance requires the integration of multiple physiological and psychological systems, working together to regulate exercise intensity in a way that will reduce time taken or increase work done. The systems that ultimately limit performance of the task are hotly contested, and may depend on a variety of factors including the type of task, the environment, external influences, training status of the individual and a host of psychological constructs. These factors can be studied in isolation, or inclusively as a whole-body or integrative system. A reductionist approach has traditionally been favoured, leading to a greater understanding and emphasis on muscle and cardio…

2015

To investigate the effect of nitric oxide on tumor development, we established a rat tumor xenograft model in zebrafish embryos. The injected tumor cells formed masses in which nitric oxide production could be detected by the use of the cell-permeant DAF-FM-DA (diaminofluorophore 4-amino-5-methylamino-2’-7’-difluorofluorescein diacetate) and DAR-4M-AM (diaminorhodamine-4M). This method revealed that nitric oxide production could be co-localized with the tumor xenograft in 46% of the embryos. In 85% of these embryos, tumors were vascularized and blood vessels were observed on day 4 post injection. Furthermore, we demonstrated by qRT-PCR that the transplanted glioma cells highly expressed Nos…