Search results for "DAMAGE"
showing 10 items of 1289 documents
Detection of primary DNA damage: applicability to biomonitoring of genotoxic occupational exposure and in clinical therapy
1995
The biological effect of putative genotoxic chemicals in the work place environment was monitored in peripheral mononuclear blood cells of exposed workers. DNA strand breaks, alkali-labile sites of DNA and DNA cross-links were measured using the alkaline filter elution method. A dose dependent increase in DNA damage was found in sterilization workers exposed to ethylene oxide and metal workers with exposure towards N-nitrosodiethanolamine. Two subpopulations with different response to the external exposure were found in nonsmoking sterilization workers. Nurses handling antineo-plastic agents without adequate safety provisions showed a statistically significantly higher rate of DNA strand br…
Analysis of DNA single-strand breaks in human venous blood: a technique which does not require isolation of white blood cells.
1997
For DNA strand break analysis in human white blood cells, usually metrizoate-Ficoll centrifugation is used to isolate mononuclear cells. This procedure is time-consuming and requires at least 20 ml of blood per sample. Therefore, we developed a technique which does not require isolation of white blood cells prior to DNA strand break analysis by alkaline elution (direct method). The sensitivity of this new technique was compared to that of the standard method, which includes isolation of mononuclear blood cells. A statistically significant increase in sensitivity was observed using the direct method. After in vitro gamma-irradiation of venous blood, an increase in the elusion rate of 7.7 × 1…
Recombinant expression of human microsomal epoxide hydrolase protects V79 Chinese hamster cells from styrene oxide- but not from ethylene oxide-induc…
1997
Styrene 7,8-oxide and ethylene oxide are widely used genotoxic bulk chemicals, which have been associated with potential carcinogenic hazard for occupationally exposed workers. Both epoxides alkylate DNA preferentially at the N-7 position of guanine and consequently produce single-strand breaks and alkali labile sites in the DNA of exposed cells. In order to study the role of human microsomal epoxide hydrolase (hmEH) in protecting cells against genotoxicity of styrene 7,8-oxide and ethylene oxide, we expressed the cDNA of hmEH in V79 Chinese hamster cells. We obtained a number of cell clones that expressed functionally active epoxide hydrolase. Among these, the clone 92hmEH-V79 revealed an …
Novel mechanism for the radiation-induced bystander effect: nitric oxide and ethylene determine the response in sponge cells.
2006
Until now the bystander effect had only been described in vertebrates. In the present study the existence of this effect has been demonstrated for the phylogenetically oldest metazoan phylum, the Porifera. We used the demosponge Suberites domuncula for the experiments in the two-chamber-system. The lower dish contained irradiated "donor" cells (single cells) and the upper dish the primmorphs ("recipient" primmorphs). The "donor" cells were treated with UV-B light (40 mJ/cm2) and 100 microM hydrogen peroxide (H2O2), factors that exist also in the natural marine aquatic environment of sponges; these factors caused a high level of DNA strand breaks followed by a reduced viability of the cells.…
I criteri di determinazione del danno nelle model rules e nel diritto comune europeo
2011
The article describes the many criteria that actually determine recoverable damages in contract, analysing similarities and differences among European legal systems and in Model Rules. In the second part, it identifies shared criteria and verify how they can be translated in a future European contract law.
Il danno da perdita dell'animale di affezione e le nuove frontiere del danno esistenziale
2023
Il saggio propone una riflessione sulla discussa fattispecie del danno da perdita dell’animale di affezione e sulle nuove frontiere del danno esistenziale, riconfigurato in termini di danno evento. La riflessione, alla luce delle nuove prospettive di tutela degli animali quali esseri sensienti, è suggerita dal nuovo articolo 13 del Trattato UE e dal recente inserimento della protezione degli animali nel nuovo testo dell’art. 9 della Costituzione. Nel caso del danno da perdita dell’animale di affezione, la violazione del diritto della persona viene riconosciuta attraverso una rilettura dell’art. 2 della Costituzione, sganciata dal vincolo formale di una previsione esplicita ed aperta all’ ev…
Enhancing formability of aluminium alloys by superimposing hydrostatic pressure
2003
Publisher Summary One of the strategic topics in manufacturing engineering is represented by the reduction of components weight. This aim is pursued by utilizing accurate and effective design tools and using lightweight metals such as aluminum, magnesium, and titanium alloys. Unfortunately, such materials often show a poor ductility, and thus enhancing formability is nowadays one of the most relevant research focus, as well as the development of effective and reliable predictive models of defects insurgence during forming processes. In this scenario, forming by means of superimposed hydrostatic pressure represents a promising alternative manufacturing technique. The chapter discusses the si…
Histone post-translational modifications as potential therapeutic targets for pain management
2021
Effective pharmacological management of pain associated with tissue pathology is an unmet medical need. Transcriptional modifications in nociceptive pathways are pivotal for the development and the maintenance of pain associated with tissue damage. Accumulating evidence has shown the importance of the epigenetic control of transcription in nociceptive pathways via histone post-translational modifications (PTMs). Hence, histone PTMs could be targets for novel effective analgesics. Here, we discuss the current understanding of histone PTMs in the modulation of gene expression affecting nociception and pain phenotypes following tissue injury. We also provide a critical view of the translationa…
Numerical modelling of low velocity multi-impact test on different stacking sequences
2019
The use of composite materials in the industrial field has been growing significantly for many years. This increase is explained by the fact that the industry, especially aeronautics which was pioneering in this field, is interested in the lightening of structures in order to be able to take up the new environmental challenges. Whether in the aeronautical field or in the land transport sector, structural elements may undergo multi-impactsor impact. Even at low velocitypose a threat to the integrity of the material and the structure itself. The objective of this work is to propose a numerical model to determine the damage within a composite structure subjected to low energy multi-impact. The…
The impact of dehydration rate on the production and cellular location of reactive oxygen species in an aquatic moss.
2012
† Background and Aims The aquatic moss Fontinalis antipyretica requires a slow rate of dehydration to survive a desiccation event. The present work examined whether differences in the dehydration rate resulted in corresponding differences in the production of reactive oxygen species (ROS) and therefore in the amount of cell damage. † Methods Intracellular ROS production by the aquatic moss was assessed with confocal laser microscopy and the ROS-specific chemical probe 2,7-dichlorodihydrofluorescein diacetate. The production of hydrogen peroxide was also quantified and its cellular location was assessed. † Key Results The rehydration of slowly dried cells was associated with lower ROS produc…