Search results for "DAS"
showing 10 items of 4164 documents
Physico-Chemical Characterization of an Exocellular Sugars Tolerant Β-Glucosidase from Grape Metschnikowia pulcherrima Isolates
2023
A broad variety of microorganisms with useful characteristics in the field of biotechnology live on the surface of grapes; one of these microorganisms is Metschnikowia pulcherrima. This yeast secretes a β-glucosidase that can be used in fermentative processes to liberate aromatic compounds. In this work, the synthesis of an exocellular β-glucosidase has been demonstrated and the optimal conditions to maximize the enzyme’s effectiveness were determined. There was a maximum enzymatic activity at 28 °C and pH 4.5. Furthermore, the enzyme presents a great glucose and fructose tolerance, and to a lesser extent, ethanol tolerance. In addition, its activity was stimulated by ca…
V2O5 nanowires with an intrinsic peroxidase-like activity
2010
V2O5 nanowires exhibit an intrinsic catalytic activity towards classical peroxidase substrates such as 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 3,3,5,5,-tetramethylbenzdine (TMB) in the presence of H2O2. These V2O5 nanowires show an optimum reactivity at a pH of 4.0 and the catalytic activity is dependent on the concentration. The Michaelis-Menten kinetics of the ABTS oxidation over these nanowires reveals a behavior similar to that of their natural vanadium-dependent haloperoxidase (V-HPO) counterparts. The V2O5 nanowires mediate the oxidation of ABTS in the presence of H2O2 with a turnover frequency (k(cat)) of 2.5 x 10(3) s(-1). The K-M values of the V2O5 nanowire…
Constitutive and regulated α-secretase cleavage of Alzheimer’s amyloid precursor protein by a disintegrin metalloprotease
1999
Amyloid β peptide (Aβ), the principal proteinaceous component of amyloid plaques in brains of Alzheimer’s disease patients, is derived by proteolytic cleavage of the amyloid precursor protein (APP). Proteolytic cleavage of APP by a putative α-secretase within the Aβ sequence precludes the formation of the amyloidogenic peptides and leads to the release of soluble APPsα into the medium. By overexpression ofa disintegrinandmetalloprotease (ADAM), classified as ADAM 10, in HEK 293 cells, basal and protein kinase C-stimulated α-secretase activity was increased severalfold. The proteolytically activated form of ADAM 10 was localized by cell surface biotinylation in the plasma membrane, but the m…
Down-regulation of Endogenous Amyloid Precursor Protein Processing due to Cellular Aging
2005
Processing of amyloid precursor protein (APP) is a well acknowledged central pathogenic mechanism in Alzheimer disease. However, influences of age-associated cellular alterations on the biochemistry of APP processing have not been studied in molecular detail so far. Here, we report that processing of endogenous APP is down-regulated during the aging of normal human fibroblasts (IMR-90). The generation of intracellular APP cleavage products C99, C83, and AICD gradually declines with increasing life span and is accompanied by a reduced secretion of soluble APP (sAPP) and sAPPalpha. Further, the maturation of APP was reduced in senescent cells, which has been shown to be directly mediated by a…
Construction of a genetically modified wine yeast strain expressing the Aspergillus aculeatus rhaA gene, encoding an -L-Rhamnosidase of enological in…
2003
Monoterpenes such as geraniol, linalool, and -terpineol present in grapes determine the varietal flavor properties of young quality wines made from Muscat varieties (for reviews, see references 19 and 21). Geraniol and linalool are considered to be the most important of the monoterpene alcohols, as they are present in greater concentrations and have lower flavor thresholds than other major wine monoterpenes. In particular, linalool is thought to be responsible for the grapelike aroma of wines produced from the Muscat variety. A large proportion of
Naudas atmazgāšana būtība, shēmas un ietekme uz Eiropas ekonomiku
2022
Mūsdienu ekonomikas attīstību ietekmē daudzi faktori. Jebkurai valstij ir aktīvi jāpiedalās noziedzīgi iegūtu līdzekļu legalizācijas un terorisma finansēšanas novēršanā, lai saglabātu veselīgu ekonomisko vidi. Naudas atmazgāšana un terorisma finansēšana ir plaši atzītas par divām vissvarīgākajām pasaules mēroga problēmām, kas rada nopietnus draudus visām valstīm. Naudas atmazgāšana tiek veikta par labu tādām nelikumīgām darbībām kā terorismu, korupciju, narkotiku un cilvēku tirdzniecību. Oficiālā statistika neaptver visas ekonomiskās darbības, kas radušās naudas atmazgāšanas rezultātā, tādējādi kaitējot valstu ekonomikai kopumā. Bakalaura nosaukums ir naudas atmazgāšana būtība, shēmas un ie…
An alternative conception of PM10 concentration changes after short-term precipitation in urban environment
2018
Abstract In the article, a linear model is presented which describes a reduction of PM10 mass concentration in relation to the type of precipitation and water vapour content in the air. The model was built using covariance analysis. In studies of PM10 concentration changes, the results of 247 observations were used, which were carried out in the urban area. Concentration changes were archived during short-term (30 min) convection and large-scale rainfalls. For the determination of PM10 mass concentration, the reference method was used. To describe changes in PM10 concentration in the air after precipitation, a series of linear models were created, in which the explanatory variables were: th…
Safety and immunomodulatory effects of three probiotic strains isolated from the feces of breast-fed infants in healthy adults: SETOPROB study.
2013
We previously described the isolation and characterization of three probiotic strains from the feces of exclusively breast-fed newborn infants: Lactobacillus paracasei CNCM I-4034, Bifidobacterium breve CNCM I-4035 and Lactobacillus rhamnosus CNCM I-4036. These strains were shown to adhere to intestinal mucus in vitro, to be sensitive to antibiotics and to resist biliary salts and low pH. In the present study, a multicenter, randomized, double-blind, placebo-controlled trial with 100 healthy volunteers in three Spanish cities was carried out to evaluate the tolerance, safety, gut colonization and immunomodulatory effects of these three probiotics. Volunteers underwent a 15-day washout perio…
Progetti di lessicografia onomastica dell’Atlante Linguistico della Sicilia
2022
L’Atlante Linguistico della Sicilia ha adottato, già una decina di anni fa, lo strumento del dizionario-atlante. Quando si è cominciato ad indagare il ricco universo onomastico, se ne è valutata la spendibilità anche su tre diversi corpora orali: uno relativo alle forme popolari del patrimonio toponomastico (DAToS, Dizionario-atlante toponomastico della Sicilia), uno riguardante l’inventario plurale dei soprannomi etnici (DASES, Dizionario-atlante dei soprannomi etnici in Sicilia), l’ultimo afferente al patrimonio antroponomastico popolare, individuale e familiare (DASS, Dizionario-atlante dei soprannomi di/in Sicilia). Il contributo fornisce lo stato dell’arte dei tre progetti e ne descriv…
Effect of cooking on oxalate content of pulses using an enzymatic procedure.
2003
An enzymatic method proposed for the determination of oxalate in urine is adapted for the estimation of soluble oxalate content in beans, chickpeas and lentils. Oxalates were extracted in water by refluxing for 2 h. The method is based on the oxidation of oxalate by the oxidase and the determination of the resulting hydrogen peroxide, which in presence of peroxidase, 3-methyl-2 benzotiazinolone and 3-dimethylamino benzoic, gives an indamine compound with an absorption maximum at 590 nm. The linearity (from 0.015 to 0.6 mM) of the method is adequate to the analysis of oxalate contents in pulses, and the inter-day precision of the method expressed as relative standard deviation was good (3.01…